White biotechnology report RIVM
... known for a very long time, ever since they were used for food and drink preservation, e.g., by lactic acid or alcoholic fermentation. Industrial biotechnology uses micro‐organisms (bac‐ teria, archaea, yeasts, fungi, micro‐algae) as production organisms. The aim of the technol‐ ogy is to optimise ...
... known for a very long time, ever since they were used for food and drink preservation, e.g., by lactic acid or alcoholic fermentation. Industrial biotechnology uses micro‐organisms (bac‐ teria, archaea, yeasts, fungi, micro‐algae) as production organisms. The aim of the technol‐ ogy is to optimise ...
Precise insertion and guided editing of higher plant
... also been shown to have lower rates of off target edits relative to Cas9 nucleases14, 15. Cpf1 nucleases in combination with crRNAs have been shown to generate indel mutations via non-homologous end joining repair (NHEJ) in both prokaryotic and eukaryotic systems11, 16. Recently, several of these Cp ...
... also been shown to have lower rates of off target edits relative to Cas9 nucleases14, 15. Cpf1 nucleases in combination with crRNAs have been shown to generate indel mutations via non-homologous end joining repair (NHEJ) in both prokaryotic and eukaryotic systems11, 16. Recently, several of these Cp ...
From Communication to DNA Sequencing
... • algorithm design based largely on heuristics • no optimality or performance guarantees But NP-hardness does not mean it is hopeless to be close to optimal. Can we first define optimality without regard to computational complexity? ...
... • algorithm design based largely on heuristics • no optimality or performance guarantees But NP-hardness does not mean it is hopeless to be close to optimal. Can we first define optimality without regard to computational complexity? ...
Aftermath of the Human Genome Project: an era
... the genetic field extending as far back as the Augustinian monk Gregor Mendel, with his studies of the delicately quaint pea flowers to the groundbreaking discovery of the double helix structure of DNA by Rosalind Franklin, James Watson, and Francis Crick sowed this foundation and planted its seeds ...
... the genetic field extending as far back as the Augustinian monk Gregor Mendel, with his studies of the delicately quaint pea flowers to the groundbreaking discovery of the double helix structure of DNA by Rosalind Franklin, James Watson, and Francis Crick sowed this foundation and planted its seeds ...
Topic #2: Should adults seek genome editing as a treatment for their
... Slide 3: In the past decade, scientists began to develop techniques known as “genome editing.” Genome editing allows scientists to make changes to a specific “target” site in the genome. One of the techniques that have generated the most excitement, due to its efficiency and ease of use, is called “ ...
... Slide 3: In the past decade, scientists began to develop techniques known as “genome editing.” Genome editing allows scientists to make changes to a specific “target” site in the genome. One of the techniques that have generated the most excitement, due to its efficiency and ease of use, is called “ ...
Genome editing and CRISPR Aim - Personal Genetics Education
... Slide 3: In the past decade, scientists began to develop techniques known as “genome editing.” Genome editing allows scientists to make changes to a specific “target” site in the genome. One of the techniques that have generated the most excitement, due to its efficiency and ease of use, is called “ ...
... Slide 3: In the past decade, scientists began to develop techniques known as “genome editing.” Genome editing allows scientists to make changes to a specific “target” site in the genome. One of the techniques that have generated the most excitement, due to its efficiency and ease of use, is called “ ...
Document
... Adeno-associated virus (AAV) vectors have reproducibly delivered transgenes in animal models, resulting in successful treatment and cure of numerous clinically relevant diseases. These studies have demonstrated sustained transgene expression without toxicity in mice, rats, dogs, and nonhuman primat ...
... Adeno-associated virus (AAV) vectors have reproducibly delivered transgenes in animal models, resulting in successful treatment and cure of numerous clinically relevant diseases. These studies have demonstrated sustained transgene expression without toxicity in mice, rats, dogs, and nonhuman primat ...
Efficient Screening of CRISPR/Cas9
... Maruyama et al. 2015). Lower than ideal frequencies, coupled with the fact that many desired CRISPR-induced mutations do not produce visible phenotypes, require more laborious screening by PCR, T7 endonuclease assays (Hwang et al. 2013), and/or sequencing. Since the frequency of CRISPR-induced mutat ...
... Maruyama et al. 2015). Lower than ideal frequencies, coupled with the fact that many desired CRISPR-induced mutations do not produce visible phenotypes, require more laborious screening by PCR, T7 endonuclease assays (Hwang et al. 2013), and/or sequencing. Since the frequency of CRISPR-induced mutat ...
Orthogonal gene knockout and activation with a catalytically active
... Finally, we aimed to test whether dRNAs in combination with sgRNAs could mediate orthogonal gene control (activation and knockout) using only active Cas9. We previously used CRISPR-Cas9 loss-of-function (LOF)16 and gain-of-function (GOF)11 screens to identify genetic modifiers that promote resistanc ...
... Finally, we aimed to test whether dRNAs in combination with sgRNAs could mediate orthogonal gene control (activation and knockout) using only active Cas9. We previously used CRISPR-Cas9 loss-of-function (LOF)16 and gain-of-function (GOF)11 screens to identify genetic modifiers that promote resistanc ...
AIR Inquiry
... Contains Confidential Business Information Further, in contrast to plants that are regulated under Part 340 because they (a) are genetically engineered with the use of plant pest donor organisms, recipient organisms, or vectors or vector agents, and (b) contain the inserted plant pest genetic materi ...
... Contains Confidential Business Information Further, in contrast to plants that are regulated under Part 340 because they (a) are genetically engineered with the use of plant pest donor organisms, recipient organisms, or vectors or vector agents, and (b) contain the inserted plant pest genetic materi ...
CRISPR
CRISPRs (clustered regularly interspaced short palindromic repeats) are segments of prokaryotic DNA containing short repetitions of base sequences. Each repetition is followed by short segments of ""spacer DNA"" from previous exposures to a bacterial virus or plasmid. It is pronounced ""crisper"".The CRISPR/Cas system is a prokaryotic immune system that confers resistance to foreign genetic elements such as plasmids and phages, and provides a form of acquired immunity. CRISPR spacers recognize and cut these exogenous genetic elements in a manner analogous to RNAi in eukaryotic organisms. CRISPRs are found in approximately 40% of sequenced bacteria genomes and 90% of sequenced archaea.The CRISPR/Cas system has been used for gene editing (adding, disrupting or changing the sequence of specific genes) and gene regulation in species throughout the tree of life. By delivering the Cas9 protein and appropriate guide RNAs into a cell, the organism's genome can be relatively cheaply cut at any desired location.