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Integrating Outcomes, Activities, and Assessments Carol A. Hurney, Ph.D. Associate Professor, Biology • include OBJECTIVES • write ASSESSMENTS • connect STRATEGIES • describe IMPACT Saturday & Sunday Friday Wednesday Tuesday Monday in-class activities out-of-class activities Thursday Snap Shot of Non-Majors Biology Warm-Ups Interactive Lectures Clicker Questions Class Activities Projects Exams Saturday & Sunday Friday Wednesday Tuesday Monday in-class activities out-of-class activities Thursday Assessments & Strategies Designing Instruction readings warm – ups tutorials review warm-ups customize class opening clicker questions 3 2 1 4 5 class activities interactive lecture clicker questions closing customize warm-up So what does this really look like?? readings warm – ups projects exams gbio103 – learning objectives General Education Cluster 3 Objectives – Students will • use theories theories and andmodels modelsas asunifying unifyingprinciples principlesthat thathelp helpusus understand understand natural naturalphenomena phenomenaand andmake makepredictions predictions • evaluate the credibility, use, and misuse of biological information in scientific developments and public-policy issues Cancer Unit Objectives – Students will • describe how exposure to carcinogens can lead to cancer • describe how susceptibility to cancer can be inherited • identify identify genes genes involved involvedinincell celldivision division • predict predict whether whethermutations mutationsiningenes genescould couldlead leadtotocancer cancer • connect ideas related to the causes of cancer with cancer treatments • integrate knowledge of cancer to the process of evaluating scientific resources on cancer • choose to read articles on cancer in the future • find one aspect of the cancer unit fascinating or compelling Weeks prior to this class.. Gene Promoter Same Protein No Protein Junk Gene Coding Region Hyper Protein No Protein Same Protein Broken Protein WU#22 After class … before next class.. A gene is on when RNA polymerase binds to the promoter Ribosomes start translation Cell Division occurs The protein from that gene is made You have just discovered a new protein called, topoisomerase. This protein functions as an enzyme that unwinds the DNA double helix, so it can be copied during S phase of the cell cycle. The amino acid sequence of this enzyme is shown below. Cells that contain defective versions of topoisomerase cannot divide because the cell never exits S phase. VAL-PRO-ILE-MET-VAL-ser-GLY-GLY-TRP-PHE-gln-asn-tyr-ser-lys-glu-leu-CYS-tyr-asp-asp-ILE-VAL-PROTRP-PHE-VAL-ALA-VAL-PRO (hydrophobic amino acids are in CAPS) What is the shape of topoisomerase? helix-sheet-helix-sheet-helix-sheet-helix helix-sheet-helix sheet-helix-sheet helix-sheet-sheet-helix In order to get the topoisomerase gene turned on in dividing cells, the gene for this enzyme most likely has a promoter promoter next to a coding region promoter with a transcription factor bound to it Cell A is producing growth factor and sending it to Cell B. Cell A and B are both skin cells. The growth factor gene is on in A and B After class … before A next class.. B Neither A or B The RNA polymerase gene is on in A and B A B Neither A or B The “Smart” gene (which codes for a protein used by brain cells) is on in A and B A B Neither A or B The growth factor receptor gene is on in A and B A B Neither A or B WU#22 WU#22 What is a proto-oncogene? The cancer causing version of a growth stimulating gene. The cancer causing version of a growth inhibiting gene. The normal version of a growth stimulating gene. The normal version of a growth inhibiting gene. What is a tumor suppressor gene? The cancer causing version of a growth stimulating gene. The cancer causing version of a growth inhibiting gene. The normal version of a growth stimulating gene. The normal version of a growth inhibiting gene. outside of class Interactive mini-lecture Sending Cell Mutations in Growth Factor Pathway Genes Inside Receiving Cell Relay protein in growth pathway gene: JUNK promoter A JUNK coding region B ras C D Coding Region Mutations No amino acid change: Same protein Made 1. Does not damage promoter. 2. Changes TATA box 1. Same Protein made 2. No Protein made Protein that is made has One amino acid change Now the protein could be HYPER!! Protein that is made has One amino acid change Could be BROKEN!!! CANCER!!! Mutant protein in growth pathway ras gene ras protein HYPERACTIVE PROTEIN IS IN THE GROWTH FACTOR PATHWAY... Which proteins control the cell cycle? Growth Inhibiting Factors Growth Factors Checkpoints Growth Inhibiting Factors 1 Signaling cell releases growth inhibiting factor (GIF) 2 GIF travels to receiving cell 3 GIF binds to a receptor protein 4 Receptor protein activates Relay proteins 5 Relay proteins activate Transcription Factor (TF) 6 TF travels to nucleus to turns on genes that tell cell NOT to divide! 7 In this picture, the oval protein will prevent cell division Signaling Cell Inside Receiving Cell In-Class Formative Assessment CA#21 – Growth Inhibitory Pathway In-Class Formative Assessment CA#21 – Growth Inhibitory Pathway Interactive mini-lecture Transcription factor in growth inhibition pathway gene JUNK promoter A coding region B C JUNK D No amino acid change: Same protein Made 1. Does not damage promoter. 2. Changes TATA box 1. Same Protein Still made 2. No protein made CANCER!!! Protein that is made has One amino acid change Now the protein could be HYPER!! Protein that is made has One amino acid change Now the protein could be BROKEN!!! Growth Inhibiting Pathway Broken Protein Or No Protein Can’t turn on genes that inhibit cell Interactive mini-lecture Proto-oncogene Tumor suppressor gene Gene 1 Gene 2 Gene 3 Muscle protein Growth Factor Gene Growth Inhibiting Factor Receptor Protein Gene 1 Gene 2 Gene 3 * * Oncogenes * Gene 4 RNA Polymerase Gene 4 * No Protein Muscle cell can no longer contract Hyperactive growth factor CQ Mutations in which genes could lead to cancer? A. Gene 1 and 2 B. Gene 2 and 3 C. Gene 2 D. Gene 3 E. Gene 3 and 4 Cell dies. A tale of three cells. Skin cell A is making growth factor and sending it to Skin cell B. Skin cell B is making growth inhibiting factor and sending it to Nerve Cell C. Nerve cell C is making growth factor and sending it to skin cell A. Use this information to answer questions 1-7. Which cell is not dividing? Skin Cell A Skin Cell B Nerve Cell C Both Cell A and Cell B Which cell(s) have the growth factor receptor gene on? A and B B and C C and A A only B only C only After class … before next class.. WU#23 If the growth inhibiting factor gene is mutated in skin cell B, which cells could become cancerous? A B C A, B and C ; A and B; B and C; none of them If the coding region of the growth factor receptor gene is mutated in Cell B, which cell could become cancerous? A B C B and C A and C none of the cells could become cancerous WU#23 If the promoter of growth inhibitory factor receptor gene is mutated in cell C, which cells could become cancerous? A B C B and C All cells could become cancerous. None of the cells could become cancerous. What is the function of the checkpoint protein BRCA1 protein? (As always, I want you to do some exploring and then give me your answer in your own words.) * * * summative assessments Friday Wednesday Monday formative assessments * * * unit exam Saturday & Sunday Tuesday in-class activities out-of-class activities Thursday Integrating Assessment tutorial - sources Scholarship of Teaching & Learning Impact of Giving Choices •Topics – cancer, colony collapse disorder, addiction •Value of WUs, CQs, CAs, •Value of exams & projects Choosing topics helped me learn. (n=127) •It made me feel like I was actually part of a large class Topics (n=106) •Seemed like we just ended up talking about basic biology stuff •Nice change from my other classes •Difficult to schedule things •The class didn’t choose topics I liked Having control of course points was a good idea. (n=127) Points (n=72) •Having control initiates a feeling of responsibility and ownership that causes a student to feel like they matter, which results in more effort •It was a little stressful … the fact that it lays on me to decide… what if I chose the wrong thing Interest (n=127) Confidence (n=127) Course Grades Spring 2007 n=69 ICT Grades 77.9 Fall 2007 n=67 LCT Grades 84.8 (p=0.0003) ICT Grades 81.5 (p=0.0052) Spring 2009 n=70 LCT Grades 84.9 (p=0.0005) ICT Grades 84.3 (p=0.0016) Programmatic Assessment • include OBJECTIVES • write ASSESSMENTS • connect STRATEGIES • describe IMPACT Saturday & Sunday Friday Wednesday Tuesday Monday in-class activities out-of-class activities Thursday Snap Shot of Non-Majors Biology