Download Growth Factor Pathway - the Biology Scholars Program Wiki

Integrating Outcomes,
Activities, and Assessments
Carol A. Hurney, Ph.D.
Associate Professor, Biology
• include OBJECTIVES
• write ASSESSMENTS
• connect STRATEGIES
• describe IMPACT
Saturday &
Sunday
Friday
Wednesday
Tuesday
Monday
in-class
activities
out-of-class
activities
Thursday
Snap Shot of Non-Majors Biology
Warm-Ups
Interactive Lectures
Clicker Questions
Class Activities
Projects
Exams
Saturday &
Sunday
Friday
Wednesday
Tuesday
Monday
in-class
activities
out-of-class
activities
Thursday
Assessments & Strategies
Designing Instruction
readings
warm – ups
tutorials
review warm-ups
customize class
opening
clicker questions
3
2
1
4
5
class activities
interactive lecture
clicker questions
closing
customize warm-up
So what does this really look like??
readings
warm – ups
projects
exams
gbio103 – learning objectives
General Education Cluster 3 Objectives – Students will
• use theories
theories and
andmodels
modelsas
asunifying
unifyingprinciples
principlesthat
thathelp
helpusus
understand
understand natural
naturalphenomena
phenomenaand
andmake
makepredictions
predictions
• evaluate the credibility, use, and misuse of biological information in
scientific developments and public-policy issues
Cancer Unit Objectives – Students will
• describe how exposure to carcinogens can lead to cancer
• describe how susceptibility to cancer can be inherited
• identify
identify genes
genes involved
involvedinincell
celldivision
division
• predict
predict whether
whethermutations
mutationsiningenes
genescould
couldlead
leadtotocancer
cancer
• connect ideas related to the causes of cancer with cancer treatments
• integrate knowledge of cancer to the process of evaluating scientific
resources on cancer
• choose to read articles on cancer in the future
• find one aspect of the cancer unit fascinating or compelling
Weeks prior to this class..
Gene
Promoter
Same Protein
No Protein
Junk
Gene
Coding Region
Hyper Protein
No Protein
Same Protein
Broken Protein
WU#22
After class … before
next class..
A gene is on when
RNA polymerase binds to the promoter
Ribosomes start translation
Cell Division occurs
The protein from that gene is made
You have just discovered a new protein called, topoisomerase. This protein functions as an enzyme that
unwinds the DNA double helix, so it can be copied during S phase of the cell cycle. The amino acid
sequence of this enzyme is shown below. Cells that contain defective versions of topoisomerase cannot
divide because the cell never exits S phase.
VAL-PRO-ILE-MET-VAL-ser-GLY-GLY-TRP-PHE-gln-asn-tyr-ser-lys-glu-leu-CYS-tyr-asp-asp-ILE-VAL-PROTRP-PHE-VAL-ALA-VAL-PRO (hydrophobic amino acids are in CAPS)
What is the shape of topoisomerase?
helix-sheet-helix-sheet-helix-sheet-helix
helix-sheet-helix
sheet-helix-sheet
helix-sheet-sheet-helix
In order to get the topoisomerase gene turned on in dividing cells, the gene for this enzyme most likely
has a
promoter
promoter next to a coding region
promoter with a transcription factor bound to it
Cell A is producing growth factor and sending it to Cell B. Cell A and B are both skin cells.
The growth factor gene is on in
A and B
After class … before
A
next class..
B
Neither A or B
The RNA polymerase gene is on in
A and B
A
B
Neither A or B
The “Smart” gene (which codes for a protein used by brain cells) is on in
A and B
A
B
Neither A or B
The growth factor receptor gene is on in
A and B
A
B
Neither A or B
WU#22
WU#22
What is a proto-oncogene?
The cancer causing version of a growth stimulating gene.
The cancer causing version of a growth inhibiting gene.
The normal version of a growth stimulating gene.
The normal version of a growth inhibiting gene.
What is a tumor suppressor gene?
The cancer causing version of a growth stimulating gene.
The cancer causing version of a growth inhibiting gene.
The normal version of a growth stimulating gene.
The normal version of a growth inhibiting gene.
outside of class
Interactive mini-lecture
Sending Cell
Mutations in Growth
Factor Pathway Genes
Inside
Receiving Cell
Relay protein in growth pathway gene:
JUNK
promoter
A
JUNK
coding region
B
ras
C D
Coding Region Mutations
No amino acid change:
Same protein Made
1. Does not damage
promoter.
2. Changes TATA box
1. Same Protein made
2. No Protein made
Protein that is made has
One amino acid change
Now the protein could
be HYPER!!
Protein that is made has
One amino acid change
Could be BROKEN!!!
CANCER!!!
Mutant protein in growth pathway
ras gene
ras protein
HYPERACTIVE PROTEIN
IS IN THE GROWTH FACTOR
PATHWAY...
Which proteins control the cell cycle?
Growth Inhibiting Factors
Growth Factors
Checkpoints
Growth Inhibiting Factors
1 Signaling cell releases growth
inhibiting factor (GIF)
2 GIF travels to receiving cell
3 GIF binds to a receptor protein
4 Receptor protein activates
Relay proteins
5 Relay proteins activate
Transcription Factor (TF)
6 TF travels to nucleus to turns
on genes that tell cell NOT to
divide!
7 In this picture, the oval protein
will prevent cell division
Signaling Cell
Inside
Receiving Cell
In-Class Formative
Assessment
CA#21 – Growth Inhibitory Pathway
In-Class Formative
Assessment
CA#21 – Growth Inhibitory Pathway
Interactive mini-lecture
Transcription factor in growth inhibition pathway gene
JUNK
promoter
A
coding region
B
C
JUNK
D
No amino acid change:
Same protein Made
1. Does not damage
promoter.
2. Changes TATA box
1. Same Protein Still made
2. No protein made
CANCER!!!
Protein that is made has
One amino acid change
Now the protein could
be HYPER!!
Protein that is made has
One amino acid change
Now the protein could
be BROKEN!!!
Growth Inhibiting Pathway
Broken Protein
Or
No Protein
Can’t turn on
genes that
inhibit cell
Interactive mini-lecture
Proto-oncogene
Tumor suppressor gene
Gene 1
Gene 2
Gene 3
Muscle protein
Growth Factor
Gene
Growth Inhibiting
Factor Receptor
Protein
Gene 1
Gene 2
Gene 3
*
*
Oncogenes
*
Gene 4
RNA Polymerase
Gene 4
*
No Protein
Muscle cell can
no longer
contract
Hyperactive
growth factor
CQ Mutations in which genes could lead to cancer?
A.
Gene 1 and 2
B.
Gene 2 and 3
C.
Gene 2
D.
Gene 3
E.
Gene 3 and 4
Cell dies.
A tale of three cells. Skin cell A is making growth factor and sending it to Skin cell B. Skin cell B
is making growth inhibiting factor and sending it to Nerve Cell C. Nerve cell C is making growth
factor and sending it to skin cell A. Use this information to answer questions 1-7.
Which cell is not dividing?
Skin Cell A
Skin Cell B
Nerve Cell C
Both Cell A and Cell B
Which cell(s) have the growth factor receptor gene on?
A and B
B and C
C and A
A only
B only
C only
After class … before next
class..
WU#23
If the growth inhibiting factor gene is mutated in skin cell B, which cells could become
cancerous?
A
B
C
A, B and C ; A and B; B and C; none of them
If the coding region of the growth factor receptor gene is mutated in Cell B, which cell
could become cancerous?
A
B
C
B and C
A and C
none of the cells could become cancerous
WU#23
If the promoter of growth inhibitory factor receptor gene is mutated in cell C, which cells
could become cancerous?
A
B
C
B and C
All cells could become cancerous.
None of the cells could become cancerous.
What is the function of the checkpoint protein BRCA1 protein? (As always, I want you to do
some exploring and then give me your answer in your own words.)
*
*
*
summative assessments
Friday
Wednesday
Monday
formative assessments
*
*
*
unit exam
Saturday &
Sunday
Tuesday
in-class
activities
out-of-class
activities
Thursday
Integrating Assessment
tutorial - sources
Scholarship of Teaching & Learning
Impact of Giving Choices
•Topics – cancer, colony collapse
disorder, addiction
•Value of WUs, CQs, CAs,
•Value of exams & projects
Choosing topics
helped me learn.
(n=127)
•It made me feel like I was
actually part of a large class
Topics
(n=106)
•Seemed like we just ended up
talking about basic biology stuff
•Nice change from my other
classes
•Difficult to schedule things
•The class didn’t choose topics I
liked
Having control of
course points
was a good idea.
(n=127)
Points
(n=72)
•Having control initiates a
feeling of responsibility and
ownership that causes a
student to feel like they
matter, which results in
more effort
•It was a little stressful … the
fact that it lays on me to
decide… what if I chose
the wrong thing
Interest (n=127)
Confidence
(n=127)
Course Grades
Spring
2007
n=69
ICT Grades
77.9
Fall
2007
n=67
LCT
Grades
84.8
(p=0.0003)
ICT Grades
81.5
(p=0.0052)
Spring
2009
n=70
LCT
Grades
84.9
(p=0.0005)
ICT Grades
84.3
(p=0.0016)
Programmatic Assessment
• include OBJECTIVES
• write ASSESSMENTS
• connect STRATEGIES
• describe IMPACT
Saturday &
Sunday
Friday
Wednesday
Tuesday
Monday
in-class
activities
out-of-class
activities
Thursday
Snap Shot of Non-Majors Biology