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International Elite Study Programme: MACROMOLECULAR SCIENCE BIOLOGICAL MACROMOLECULES Biological Macromolecules: „Building blocks of Life“ The advanced course on Biological Macromolecules is an introduction to various aspects of structure and function of proteins, nucleic acids, and their complexes. The topics addressed are a selection of modern biophysical methods applied to current questions in macromolecular biochemistry. In particular, the interplay of structure and function of biological macromolecules will be highlighted. Each topic is presented as a closed and independent entity. During each entity students will be introduced to selected techniques. Following, they will be shown how the techniques presented can be applied to investigate a biologically relevant question. In detail, this module will cover techniques used in modern structural biology, including nuclear magnetic resonance spectroscopy, X-ray crystallography, and computational biology / bioinformatics. Moreover, concepts of microbiology and the function of selected biological macromolecules in this context will be introduced. Four teaching entities – each lasting for 10 hours – consist of lectures, a seminar/round-table discussion, and laboratory introductions. In addition, tours to the research facilities and a visit in a biotechnological company. In this context, the transition from a research result to a marketable product will be discussed with the students. Biological Macromolecules: „Building blocks of Life“ Nucleic acids Æ Nucleotides Bases (A,G; U, C, T) + Ribose, Desoxyribose + Phosphate RNA, DNA Proteins Æ Amino acids Enzymes Storage function Supporting tissue Energy household Polysaccharides Interplay of Bio-Macromolecules: Function of an organism Æ learning how to cure malfunctions Technical Applications: Biocatalysis, Diagnostics and Sensorics, Environmental protection Summer School 2006 Biological Macromolecules: Monday, July 24th Bioinformatics and Computational Biology (Prof. Dr. Matthias Ullmann, Bioinformatics) Tuesday, July 25th Crystallography of Biomacromolecules (Dr. Holger Dobbek, Protein Crystallography) Wednesday. July 26th Structure and Dynamics: Biomolecular NMR (Prof. Dr. Paul Rösch, Department of Biopolymers) Thursday, July 27th Biomacromolecules and Microbiology (Prof. Dr. Ortwin Meyer, Department of Microbiology) Friday, July 28th Excursion and Discussion at November AG, Erlangen Location: BGI S66 Theoretical and Structural Aspects of Molecular Bioenergetics (Prof. Dr. Matthias Ullmann, Bioinformatics) 9:00 - 9:50: Short introduction into molecular bioenergetics (Matthias Ullmann) 10:00 - 10:30: Calculating protonation and oxidation probabilities of proteins (Frank Dickert) 10:30 – 11:15: Modelling charge transfer reactions in proteins (Torsten Becker) 11:15 – 11:30: break 11:30 – 12:00: Membrane Transport: Channels and Pumps (Mirco Till) 12:00 – 14:00: lunch break 14:00 – 14:30: Bacteriorhodopsin (Edda Kloppomann) 14:30 – 15:00: Structure and Mechanism of Cytochrome bc1 (Thomas Ullmann) 15:00 – 15:30: Structure and Mechanism of Cytochrome c oxidase (Punnagai Munusami) 15:30 - 16:00: break 16:00 – 17:00: Prof. Oliver Einsle (University of Göttingen): A molecular and functional view of the biological nitrogen cycle 17:00 - Open discussion and Get together Location: BGI S66 Crystallography of Biomacromolecules (Dr. Holger Dobbek, Protein Crystallography) 9.00-11:00: Lecture: Introduction to Protein Crystallography – historical and thematic perspectives (Holger Dobbek) 11.00-12.00: Practical: Computational Crystallography (Holger Dobbek) Presentation of Protein Structures Electron density and its interpretation at different resolutions Ligand binding in solution / crystal and structures of protein-ligand complexes 12.00-13.00: Lunch break 13.00-14.00: Lecture: 14:00 - 15:30: Lab. tour: X-ray diffractometer(PX-group) Modelling / interpreting electron densities in 3D Protein crystallization under oxic and anoxic conditions Protein production and isolation under oxic and anoxic conditions Transient enzyme kinetics 15:30 – 16:00 Break 16:00-17:00 Guest seminar: 17:00 - Active Crystals – Problems and Perspectives of monitoring dynamic /catalytic processes in protein crystals (Holger Dobbek) Prof. Dr. Ilme Schlichting (MPI Heidelberg) Proteins caught in the act Analysis by time-resolved crystallography Open discussion and Get together Location: BGI S66 Structure and Dynamics: Biomolecular NMR (Prof. Dr. Paul Rösch, Department of Biopolymers) 9.00-10:25: Lecture: Production of Proteins and Nucleic Acids for NMR (Birgitta Wöhrl) Study of structure and dynamics by NMR require large amounts of very pure biomacromolecules, which also need to be enriched with magnetically active, stable istotopes. 10.35-12.00: Lecture: Dynamics in Biopolymers (Stephan Schwarzinger) What biophysical methods can tell you about the motions in biomacromolecules – Application of NMR & optical spectroscopy. 12.00-13.00: Lunch break 13.00-14.30: Lecture: NMR-Facility Bayreuth (Kristian Schweimer) Demonstration, how spectra can be assigned to individual atoms, and how high-resolution structures can be determined. 14:40 - 15:30: Lab. tour: NMR-Facility Bayreuth (Kristian Schweimer) 15:30 – 16:00 Break 16:00-17:00 Guest seminar: 17:00 - Prof. Dr. Michael Sattler (EMBL Heidelberg) NMR-Studies of Molecular Recognition During RNA Interference and Splicosome Assembly Open discussion and Get together (BBQ) Location: B10, conference room Biomacromolecules and Microbiology (Prof. Dr. Orwin Meyer, Department of Microbiology) 9.30-10:00: Getting into contact at Microbiology conference room, B10 10.00-12.00: Introduction to Microbiology and Natural Polymers Round Table Discussion 12.00-13.30: Lunch break 13.30-15.00: Practical Approach to Natural Polymers and Polymer Degrading Bacteria 15:00 – 16:00 Break 16:00-17:00 Guest seminar: 17:00 - Get together Prof. Dr. William Martin (University of Düsseldorf) On the possible nature of ancient metabolism Summer School 2006 Biological Macromolecules: Monday, July 24th Bioinformatics and Computational Biology (Prof. Dr. Matthias Ullmann, Bioinformatics) Tuesday, July 25th Crystallography of Biomacromolecules (Dr. Holger Dobbek, Protein Crystallography) Wednesday. July 26th Structure and Dynamics: Biomolecular NMR (Prof. Dr. Paul Rösch, Department of Biopolymers) Thursday, July 27th Biomacromolecules and Microbiology (Prof. Dr. Ortwin Meyer, Department of Microbiology) Friday, July 28th Excursion and Discussion at November AG, Erlangen