Download BIOLOGICAL MACROMOLECULES

International Elite Study Programme:
MACROMOLECULAR SCIENCE
BIOLOGICAL MACROMOLECULES
Biological Macromolecules:
„Building blocks of Life“
The advanced course on Biological Macromolecules is an introduction to various aspects of
structure and function of proteins, nucleic acids, and their complexes. The topics addressed
are a selection of modern biophysical methods applied to current questions in macromolecular
biochemistry. In particular, the interplay of structure and function of biological macromolecules
will be highlighted. Each topic is presented as a closed and independent entity. During each
entity students will be introduced to selected techniques. Following, they will be shown how the
techniques presented can be applied to investigate a biologically relevant question. In detail,
this module will cover techniques used in modern structural biology, including nuclear magnetic
resonance spectroscopy, X-ray crystallography, and computational biology / bioinformatics.
Moreover, concepts of microbiology and the function of selected biological macromolecules in
this context will be introduced.
Four teaching entities – each lasting for 10 hours – consist of lectures, a seminar/round-table
discussion, and laboratory introductions. In addition, tours to the research facilities and a visit in
a biotechnological company. In this context, the transition from a research result to a
marketable product will be discussed with the students.
Biological Macromolecules:
„Building blocks of Life“
Nucleic acids Æ Nucleotides
Bases (A,G; U, C, T)
+ Ribose, Desoxyribose
+ Phosphate
RNA, DNA
Proteins Æ Amino acids
Enzymes
Storage function
Supporting tissue
Energy household
Polysaccharides
Interplay of Bio-Macromolecules:
Function of an organism Æ
learning how to cure malfunctions
Technical Applications:
Biocatalysis, Diagnostics and
Sensorics, Environmental protection
Summer School 2006
Biological Macromolecules:
Monday, July 24th
Bioinformatics and Computational Biology
(Prof. Dr. Matthias Ullmann, Bioinformatics)
Tuesday, July 25th
Crystallography of Biomacromolecules
(Dr. Holger Dobbek, Protein Crystallography)
Wednesday. July 26th
Structure and Dynamics: Biomolecular NMR
(Prof. Dr. Paul Rösch, Department of Biopolymers)
Thursday, July 27th
Biomacromolecules and Microbiology
(Prof. Dr. Ortwin Meyer, Department of Microbiology)
Friday, July 28th
Excursion and Discussion at November AG, Erlangen
Location: BGI S66
Theoretical and Structural Aspects of Molecular Bioenergetics
(Prof. Dr. Matthias Ullmann, Bioinformatics)
9:00 - 9:50:
Short introduction into molecular bioenergetics (Matthias Ullmann)
10:00 - 10:30:
Calculating protonation and oxidation probabilities of proteins (Frank Dickert)
10:30 – 11:15:
Modelling charge transfer reactions in proteins (Torsten Becker)
11:15 – 11:30:
break
11:30 – 12:00:
Membrane Transport: Channels and Pumps (Mirco Till)
12:00 – 14:00:
lunch break
14:00 – 14:30:
Bacteriorhodopsin (Edda Kloppomann)
14:30 – 15:00:
Structure and Mechanism of Cytochrome bc1 (Thomas Ullmann)
15:00 – 15:30:
Structure and Mechanism of Cytochrome c oxidase (Punnagai Munusami)
15:30 - 16:00:
break
16:00 – 17:00:
Prof. Oliver Einsle (University of Göttingen):
A molecular and functional view of the biological nitrogen cycle
17:00 -
Open discussion and Get together
Location: BGI S66
Crystallography of Biomacromolecules
(Dr. Holger Dobbek, Protein Crystallography)
9.00-11:00:
Lecture:
Introduction to Protein Crystallography –
historical and thematic perspectives (Holger Dobbek)
11.00-12.00:
Practical: Computational Crystallography (Holger Dobbek)
Presentation of Protein Structures
Electron density and its interpretation at different resolutions
Ligand binding in solution / crystal and structures of protein-ligand complexes
12.00-13.00:
Lunch break
13.00-14.00:
Lecture:
14:00 - 15:30:
Lab. tour: X-ray diffractometer(PX-group)
Modelling / interpreting electron densities in 3D
Protein crystallization under oxic and anoxic conditions
Protein production and isolation under oxic and anoxic conditions
Transient enzyme kinetics
15:30 – 16:00
Break
16:00-17:00
Guest seminar:
17:00 -
Active Crystals – Problems and Perspectives of monitoring
dynamic /catalytic processes in protein crystals (Holger Dobbek)
Prof. Dr. Ilme Schlichting (MPI Heidelberg)
Proteins caught in the act Analysis by time-resolved crystallography
Open discussion and Get together
Location: BGI S66
Structure and Dynamics: Biomolecular NMR
(Prof. Dr. Paul Rösch, Department of Biopolymers)
9.00-10:25:
Lecture:
Production of Proteins and Nucleic Acids for NMR (Birgitta Wöhrl)
Study of structure and dynamics by NMR require large amounts
of very pure biomacromolecules, which also need to be enriched
with magnetically active, stable istotopes.
10.35-12.00:
Lecture:
Dynamics in Biopolymers (Stephan Schwarzinger)
What biophysical methods can tell you about the motions in biomacromolecules – Application of NMR & optical spectroscopy.
12.00-13.00:
Lunch break
13.00-14.30:
Lecture:
NMR-Facility Bayreuth (Kristian Schweimer)
Demonstration, how spectra can be assigned to individual atoms,
and how high-resolution structures can be determined.
14:40 - 15:30:
Lab. tour: NMR-Facility Bayreuth (Kristian Schweimer)
15:30 – 16:00
Break
16:00-17:00
Guest seminar:
17:00 -
Prof. Dr. Michael Sattler (EMBL Heidelberg)
NMR-Studies of Molecular Recognition During
RNA Interference and Splicosome Assembly
Open discussion and Get together (BBQ)
Location: B10, conference room
Biomacromolecules and Microbiology
(Prof. Dr. Orwin Meyer, Department of Microbiology)
9.30-10:00:
Getting into contact at Microbiology conference room, B10
10.00-12.00:
Introduction to Microbiology and Natural Polymers
Round Table Discussion
12.00-13.30:
Lunch break
13.30-15.00:
Practical Approach to Natural Polymers and Polymer Degrading Bacteria
15:00 – 16:00
Break
16:00-17:00
Guest seminar:
17:00 -
Get together
Prof. Dr. William Martin (University of Düsseldorf)
On the possible nature of ancient metabolism
Summer School 2006
Biological Macromolecules:
Monday, July 24th
Bioinformatics and Computational Biology
(Prof. Dr. Matthias Ullmann, Bioinformatics)
Tuesday, July 25th
Crystallography of Biomacromolecules
(Dr. Holger Dobbek, Protein Crystallography)
Wednesday. July 26th
Structure and Dynamics: Biomolecular NMR
(Prof. Dr. Paul Rösch, Department of Biopolymers)
Thursday, July 27th
Biomacromolecules and Microbiology
(Prof. Dr. Ortwin Meyer, Department of Microbiology)
Friday, July 28th
Excursion and Discussion at November AG, Erlangen