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The Cell Membrane AP Biology 2007-2008 Membrane is a collage of proteins & other molecules embedded in the fluid matrix of the lipid bilayer Glycoprotein Extracellular fluid Glycolipid Phospholipids Cholesterol Peripheral protein AP Biology Transmembrane proteins Cytoplasm Filaments of cytoskeleton 1972, S.J. Singer & G. Nicolson proposed Fluid Mosaic Model Phospholipids Phosphate head “attracted to water” hydrophilic Fatty acid tails Phosphate hydrophobic Arranged as a bilayer Fatty acid “repelled by water” Aaaah, one of those structure–function examples AP Biology Cell membrane defines cell Cell membrane separates living cell from aqueous environment thin barrier = 8nm thick Controls traffic in & out of the cell allows some substances to cross more easily than others hydrophobic (nonpolar) vs. hydrophilic (polar) AP Biology Arranged as a Phospholipid bilayer Serves as a cellular barrier / border sugar H 2O salt polar hydrophilic heads nonpolar hydrophobic tails impermeable to polar molecules polar hydrophilic heads waste AP Biology lipids Permeability to polar molecules? Membrane becomes semi-permeable via protein channels specific channels allow specific material across cell membrane inside cell NH AP Biology 3 salt H 2O aa sugar outside cell Cell membrane must be more than lipids… In 1972, S.J. Singer & G. Nicolson proposed that membrane proteins are inserted into the phospholipid bilayer AP Biology Membrane is a collage of proteins & other molecules embedded in the fluid matrix of the lipid bilayer Glycoprotein Extracellular fluid Glycolipid Phospholipids Cholesterol Peripheral protein AP Biology Transmembrane proteins Cytoplasm Filaments of cytoskeleton 1972, S.J. Singer & G. Nicolson proposed Fluid Mosaic Model Cell membrane is more than lipids… Transmembrane proteins embedded in phospholipid bilayer Ideal because of multiple functional groups within the same protein create semi-permeable channels lipid bilayer membrane AP Biology protein channels in lipid bilyer membrane Many Functions of Membrane Proteins “Channel” Outside Plasma membrane Inside Transporter Enzyme activity Cell surface receptor Cell adhesion Attachment to the cytoskeleton “Antigen” AP Biology Cell surface identity marker Membrane Proteins Proteins determine membrane’s specific functions cell membrane & organelle membranes each have unique collections of proteins Classes of membrane proteins: peripheral proteins loosely bound to surface of membrane ex: cell surface identity marker (antigens) integral proteins penetrate lipid bilayer, usually across whole membrane transmembrane protein ex: transport proteins channels, permeases (pumps) AP Biology Proteins domains anchor molecule Within membrane Polar areas of protein nonpolar amino acids hydrophobic anchors protein into membrane On outer surfaces of membrane in fluid polar amino acids hydrophilic extend into AP Biology extracellular fluid & into cytosol Nonpolar areas of protein + H H+ Examples Retinal chromophore NH2 aquaporin = water channel in bacteria Porin monomer H 2O b-pleated sheets Bacterial outer membrane Nonpolar (hydrophobic) a-helices in the cell membrane COOH H++ H Cytoplasm proton pump channel in photosynthetic bacteria H O AP Biology 2 function through conformational change = protein changes shape Membrane carbohydrates Play a key role in cell-cell recognition ability of a cell to distinguish one cell from another antigens important in organ & tissue development basis for rejection of foreign cells by immune system AP Biology Movement across the Cell Membrane AP Biology Diffusion 2nd Law of Thermodynamics governs biological systems universe tends towards disorder (entropy) Diffusion AP Biology movement from HIGH LOW concentration Simple Diffusion Move from HIGH to LOW concentration “passive transport” no energy needed AP Biology diffusion movement of water osmosis Facilitated Diffusion Diffusion through protein channels channels move specific molecules across cell membrane facilitated = with help no energy needed open channel = fast transport HIGH LOW AP Biology “The Bouncer” Active Transport Cells may need to move molecules against concentration gradient conformational shape change transports solute from one side of membrane to other protein “pump” “costs” energy = ATP LOW conformational change ATP HIGH AP Biology “The Doorman” Active transport Many models & mechanisms ATP AP Biology ATP antiport symport Transport Summary Passive Transport Simple diffusion diffusion of nonpolar, hydrophobic molecules lipids HIGH LOW concentration gradient Facilitated transport diffusion of polar, hydrophilic molecules through a protein channel HIGH LOW concentration gradient Active transport diffusion against concentration gradient LOW HIGH AP Biology uses a protein pump requires ATP ATP Transport summary simple diffusion facilitated diffusion active transport AP Biology ATP How about large molecules? Moving large molecules into & out of cell through vesicles & vacuoles endocytosis phagocytosis = “cellular eating” pinocytosis = “cellular drinking” AP Biology exocytosis exocytosis Endocytosis phagocytosis fuse with lysosome for digestion pinocytosis non-specific process receptor-mediated endocytosis triggered by molecular signal AP Biology The Special Case of Water Movement of water across the cell membrane AP Biology 2007-2008 Osmosis is just diffusion of water Diffusion of water from HIGH concentration of water to LOW concentration of water AP Biology across a semi-permeable membrane Concentration of water Direction of osmosis is determined by comparing total solute concentrations Hypertonic - more solute, less water Hypotonic - less solute, more water Isotonic - equal solute, equal water water AP Biology hypotonic hypertonic net movement of water Managing water balance Cell survival depends on balancing water uptake & loss AP Biology freshwater balanced saltwater 1 Managing water balance Hypotonic a cell in fresh water high concentration of water around cell problem: cell gains water, swells & can burst KABOOM! example: Paramecium ex: water continually enters Paramecium cell solution: contractile vacuole pumps water out of cell ATP ATP plant cells No problem, here turgid = full cell wall protects from bursting AP Biology freshwater Pumping water out Contractile vacuole in Paramecium ATP AP Biology 2 Managing water balance Hypertonic I’m shrinking, a cell in salt water I’m shrinking! low concentration of water around cell problem: cell loses water & can die example: shellfish solution: take up water or pump out salt I plant cells will survive! plasmolysis = wilt can recover AP Biology saltwater 3 Managing water balance Isotonic That’s perfect! animal cell immersed in mild salt solution no difference in concentration of water between cell & environment problem: none no net movement of water flows across membrane equally, in both directions I could cell in equilibrium be better… volume of cell is stable example: blood cells in blood plasma slightly salty IV solution in hospital AP Biology balanced Animation 1991 | 2003 Aquaporins Water moves rapidly into & out of cells evidence that there were water channels protein channels allowing flow of water across cell membrane AP Biology Peter Agre Roderick MacKinnon John Hopkins Rockefeller Do you understand Osmosis… .05 M .03 M Cell (compared to beaker) hypertonic or hypotonic Beaker (compared to cell) hypertonic or hypotonic Which way does the water flow? in or out of cell AP Biology Used to describe the movement of water into & out of a cell Water potential = pressure potential + solute potential Pressure Potential In plant cell, pressure exerted by rigid cell wall that prevents further water uptake Effect of solute concentration Pure water at atmospheric pressure has solute potential of 0 Solute Potential As solute is added, value for solute potential becomes more negative Causes water potential to decrease As solute is added, water potential drops and water tends to move into solution AP Biology • • • • 𝝋s = -iCRT i = ionization constant C=Molar concentration R=Pressure constant T=Temp in Kelvin AP Biology The molar concentration of a sugar solution in an open beaker has been determined to be 0.3M. Calculate the solute potential at 27°C. Round your answer to the nearest hundredth 𝝋s = -iCRT 𝝋s = -(1) (0.3M) (0.0831) (27 + 273) 𝝋s = -7.479 AP Biology The molar concentration of a sugar solution in an open beaker has been determined to be 0.3M. Calculate the solute potential at 27°C. Round your answer to the nearest hundredth 𝝋s = -iCRT 𝝋s = -(1) (0.3M) (0.0831) (27 + 273) 𝝋s = -7.479 AP Biology The pressure potential of a solution open to the air is zero. Since you know the solute potential of the solution, you can now calculate the water potential. What is the water potential for this example? Round your answer to the nearest hundredth. 𝝋 = 𝝋𝒑 + 𝝋𝒔 𝝋 = 𝟎 + (−𝟕. 𝟒𝟕𝟗) 𝝋 = −𝟕. 𝟒𝟕𝟗 AP Biology The pressure potential of a solution open to the air is zero. Since you know the solute potential of the solution, you can now calculate the water potential. What is the water potential for this example? Round your answer to the nearest hundredth. 𝝋 = 𝝋𝒑 + 𝝋𝒔 𝝋 = 𝟎 + (−𝟕. 𝟒𝟕𝟗) 𝝋 = −𝟕. 𝟒𝟕𝟗 AP Biology Calculate water potential for each Describe what will need to occur for equilibrium to be reached. AP Biology Water Potential water potential of pure water in an open container is zero no solute and pressure in container is zero Adding solute lowers water potential. When a solution is enclosed by rigid cell wall, movement of water into cell will exert pressure on cell wall. increases in pressure within cell will raise the water potential. AP Biology Any Questions?? AP Biology