Download VE-cadherin (C-19): sc-6458

SANTA CRUZ BIOTECHNOLOGY, INC.
VE-cadherin (C-19): sc-6458
The Power to Question
BACKGROUND
APPLICATIONS
Ca2+-dependent
The cadherins are a family of
adhesion molecules that
function to mediate cell-cell binding critical to the maintenance of tissue
structure and morphogenesis. Cadherins each contain a large extracellular
domain at the amino terminus, which is characterized by a series of five
homologous repeats, the most distal of which is thought to be responsible
for binding specificity. The relatively short carboxy terminal, intracellular
domain interacts with a variety of cytoplasmic proteins, including β-catenin,
to regulate cadherin function. VE-cadherin (for vascular endothelial-cadherin,
also designated cadherin-5) is localized at intercellular junctions of endothelial
cells, where it is thought to play a role in the cohesion and organization of
intercellular junctions.
VE-cadherin (C-19) is recommended for detection of VE-cadherin of mouse,
rat and human origin by Western Blotting (starting dilution 1:200, dilution
range 1:100-1:1000), immunoprecipitation [1–2 µg per 100–500 µg of total
protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50,
dilution range 1:50-1:500) and flow cytometry (1 µg per 1 x 106 cells).
Suitable for use as control antibody for VE-cadherin siRNA (h): sc-36814
and VE-cadherin siRNA (m): sc-36813.
Molecular Weight of VE-cadherin: 130 kDa.
Positive Controls: rat placenta extract or HUV-EC-C whole cell lysate.
DATA
REFERENCES
1. Takeichi, M. 1988. The cadherins: cell-cell adhesion molecules controlling
animal morphogenesis. Development 102: 639-655.
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90 K
2. Hatta, M., et al. 1991. Genomic organization and chromosomal mapping of
the mouse P-cadherin gene. Nucl. Acids Res. 19: 4437-4441.
55 K
3. Koch, P.J., et al. 1994. Desmosomal cadherins: another growing multigene
family of adhesion molecules. Curr. Opin. Cell Biol. 6: 682-687.
4. Ranscht, B. 1994. Cadherins and catenins: interactions and functions in
embryonic development. Curr. Opin. Cell Biol. 6: 740-746.
5. Ayalon, O., et al. 1994. Spatial and temporal relationships between
cadherins and PECAM-1 in cell-cell junctions of human endothelial cells.
J. Cell Biol. 126: 247-258.
< VE-cadherin
43 K
VE-cadherin (C-19): sc-6458. Western blot analysis of
VE-cadherin expression in rat placenta extract.
SELECT PRODUCT CITATIONS
6. Takeichi, M. 1995. Morphogenetic roles of classic cadherins. Curr. Opin.
Cell Biol. 7: 619-627.
1. Tinsley, J.H., et al. 1999. Activated neutrophils induce hyperpermeability
and phosphorylation of adherens junction proteins in coronary venular
endothelial cells. J. Biol. Chem. 274: 24930-24934.
7. Brevario, F., et al. 1995. Functional properties of human vascular endothelial
cadherin (7B4/cadherin-5), an endothelium-specific cadherin. Arterioscler.
Thromb. Vasc. Biol. 15: 1229-1239.
2. Ukropec, J.A., et al. 2000. SHP2 association with VE-cadherin complexes
in human endothelial cells is regulated by thrombin. J. Biol. Chem. 275:
5983-5986.
CHROMOSOMAL LOCATION
3. Ozaki, M., et al. 2002. Overexpression of endothelial nitric oxide synthase in endothelial cells is protective against ischemia-reperfusion
injury in mouse skeletal muscle. Am. J. Pathol. 160: 1335-1344.
Genetic locus: CDH5 (human) mapping to 16q22.1; Cdh5 (mouse) mapping
to 8 D3.
SOURCE
4. Shay-Salit, A., et al. 2002. VEGF receptor 2 and the adherens junction as
a mechanical transducer in vascular endothelial cells. Proc. Natl. Acad.
Sci. USA 99: 9462-9467.
VE-cadherin (C-19) is an affinity purified goat polyclonal antibody raised
against a peptide mapping at the C-terminus of VE-cadherin of human origin.
5. Wu, W., et al. 2003. VEGF receptor expression and signaling in human
bladder tumors. Oncogene 22: 3361-3370.
PRODUCT
6. Potter, M., et al. 2005. Tyrosine phosphorylation of VE-cadherin prevents
binding of p120- and β-catenin and maintains the cellular mesenchymal
state. J. Biol. Chem. 280: 31906-31912.
Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and
0.1% gelatin.
Blocking peptide available for competition studies, sc-6458 P, (100 µg peptide
in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA).
Available as phycoerythrin conjugate for flow cytometry, sc-6458 PE, 100 tests.
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
Santa Cruz Biotechnology, Inc.
1.800.457.3801
831.457.3800
fax 831.457.3801
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