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Glucose Metabolism
The metabolism of glucose is central to mammalian life. Dynamic changes in any of the steps involved in processing
glucose and its derivatives contribute to a wide range of diseases. Measuring the enzymes and metabolites is pivotal to
biological and medical research. Cayman offers an array of tools to make these measurements quickly, easily and accurately.
Glucose
Glucose
Glucose Uptake
Glucose
ATP
HK
ADP
NADP+
Glucose-6-Phosphate
Glucose
Glucose Uptake
ATP
ADP
aldolase
aldolase
IDH
CO2
CoA-SH
NADH
Citrate
2NAD+
2
Malate
PGK
2NADH
Citrate
2ATP
2ADP
Citrate
NADH
H2O
enolase
2ADP
NAD+
Pyruvate
PGM
PK
Pyruvate (2X)
enolase
Lactate Dehydrogenase
2NADH
NAD+
Pyruvate
CoA-SH
MDH
2ATP
CoA-SH
NADH
Acetyl-CoA
Oxaloacetate
PGK
PGM
2ATP
CoA-SH
2
fumarase
GAPDH
2ADP
Succinate
Dehydrogenase
Electron
Transport Fumarate
Chain
O
GAPDH
2NAD+
Acetyl-CoA
SynthaseOxaloacetate
CoA-SH Electron
Fumarate
fumarase
Transport NADH
CoA-SH
MDH
Malate
Chain
O
Succinate
aldolase
aldolase
Synthase
Succinyl-CoA
Succinate Succinate
Succ-CoA Synthetase Dehydrogenase
GTP
TIM
TIM
2NADH
Citrate
Aconitase
CoA-SH
GTP
Fructose-1,6-Bisphosphate
Aconitase
Succinyl-CoA
CoA-SH Succ-CoA Synthetase
CO2
Fructose-1,6-Bisphosphate
Isocitrate
α-Ketoglutarate
α-KGDH
PFK
Isocitrate
α-Ketoglutarate
6-Phosphogluconolactone
PFK
ADP
α-KGDH
6-Phosphogluconolactone
NADPH
Fructose-6-Phosphate
ATP
IDH
NADP+
Glucose-6-Phosphate
Fructose-6-Phosphate
Glucose-6-P-Dehydrogenase
PGI
ADP
CO2
Glucose-6-P-Dehydrogenase
PGI
HK
ATP
CO2
NADPH
H2O
2ADP
2NAD+
PK
Lactate (2X)
2ATP
Pyruvate (2X)
Lactate Dehydrogenase
2NADH
2NAD+
Lactate (2X)
Assay Kits
Item No.
Product Name
Item No.
Product Name
600470
Glucose Uptake Cell-Based Assay Kit
700510
L-Lactate Assay Kit
10009582
Glucose Colorimetric Assay Kit
700470
Pyruvate Assay Kit
600450
Glycolysis Cell-Based Assay Kit
700480
Glycogen Assay Kit
601060
Oxygen Consumption/Glycolysis Dual Assay Kit
601450
Mitochondrial Stress Test Kit
700750
Glucose-6-Phosphate Fluorometric Assay Kit
600800
700300
Glucose-6-Phosphate Dehydrogenase Activity Assay Kit
Oxygen Consumption Rate Assay Kit
(MitoXpress® Xtra HS Method)
600880
Oxygen Consumption/MitoMembrane Potential Dual Assay Kit
601230
pH-Xtra
700410
ATP Detection Assay Kit - Luminescence
TM
Glycolysis Cell-Based Assay Kit
View a complete list of our metabolism products at www.caymanchem.com
2017
1180 EAST ELLSWORTH ROAD · ANN ARBOR, MI 48108 · USA · [800] 364-9897
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Glucose Metabolism Substrates
Item No.
Product Name
Activity
20516
D-Fructose-1,6-bisphosphate
(sodium salt hydrate)
An intermediate in the glycolysis and gluconeogenesis pathways formed by the phosphorylation of
fructose-6-phosphate by phosphofructokinase
19588
D-Fructose-6-phosphate (sodium salt)
An intermediate of the glycolytic pathway formed by the isomerization of glucose-6-phosphate
16464
α-D-Glucose-1,6-biphosphate
(cyclohexyl ammonium salt hydrate)
A derivative of glucose used to study carbohydrate metabolism
20376
D-Glucose-6-phosphate (sodium salt)
The starting molecule of both glycolysis and the pentose phosphate pathways
19192
Phosphoenolpyruvic Acid (potassium salt)
An enzyme substrate for both glycolysis and gluconeogenesis pathways
21423
D-Ribulose-5-phosphate (sodium salt)
An intermediate in the pentose phosphate pathway
21344
D-Sedoheptulose-7-phosphate (barium salt)
An intermediate in the pentose phosphate pathway
Glucose Metabolism Inhibitors
Altered glucose metabolism is characteristic of neoplastic and highly proliferative cells. Inhibitors of the rate-controlling enzymes in
the gluconeogenesis and glycolysis pathways have great potential in the treatment of cancer.
Item No.
Product Name
Activity
10009315
6-Aminonicotinamide
Inhibits 6-PGD (Ki = 0.46 μM); interferes with glycolysis, resulting in ATP depletion and synergizes
with DNA-crosslinking chemotherapy drugs, like cisplatin, in killing cancer cells
18860
Fructose-1,6-bisphosphatase-1 Inhibitor
Blocks fructose-1,6-bisphosphatase-1 activity (IC50 = 3.4 µM; Ki = 1.1 µM); blocks glucose production
in starved rat hepatoma cells (IC50 = 6.6 µM)
14079
Heptelidic Acid
Inhibits GAPDH (Ki = 1.6 µM); selectively induces apoptosis in high-glycolytic cancer cells by
inhibiting the generation of ATP in the glycolytic pathway
16548
D-Mannoheptulose
A competitive inhibitor of glucokinases and hexokinases (Ki = 0.25 mM); prevents the conversion of
glucose to glucose-6-phosphate
17689
PFK15
Inhibits PFKFB3 (IC50 = 207 nM); suppresses glucose uptake and growth of Lewis lung carcinomas
in syngeneic mice
19863
Physcion
Inhibits 6-PGD (IC50 = 38.5 µM); decreases lipogenesis and RNA biosynthesis in cancer cells
19276
3PO
Inhibits PFKFB3 (IC50 = 23 µM); causes a rapid reduction in fructose-2,6-bisphosphate, glucose
uptake, and lactate secretion
15352
YZ9
Inhibits PFKFB3 (IC50 = 183 nM in vitro); inhibits the growth of HeLa cells (GI50 = 2.7 μM)
Fluorescent Probes
Item No.
Product Name
Activity
9002314
NBD-Fructose
A fluorescently labeled fructose derivative that specifically accumulates in cells bearing GLUT5
transporters; used to monitor fructose uptake in normal and cancerous cells via fluorescent microscopy
11046
2-NBDG
A fluorescently labeled deoxyglucose analog that is used primarily to directly monitor glucose
uptake by living cells and tissues
13961
6-NBDG
A fluorescent glucose analog that is used to monitor glucose uptake and transport in living cells;
validated as a probe for the GLUT1 transporter in cells
View a complete list of our metabolism products at www.caymanchem.com
Distributed by:
2017
In Italy: Vinci-Biochem Srl
Contatto diretto: tel. 0571 568 147
[email protected]
www.vincibiochem.it
1180 EAST ELLSWORTH ROAD · ANN ARBOR, MI 48108 · USA · [800] 364-9897
WWW.CAYMANCHEM.COM