Download Hepatocyte growth factor receptor B-2837-3_2

SOMAmer® anti‐Hepatocyte growth factor receptor Reagent DESCRIPTION
SOMAmer Reagent No. Protein Target Target UniProt ID Species Reactivity 5’ Functional Mod. Target Source Formulation
B‐2837‐3_2 Hepatocyte growth factor receptor P08581 Human Biotinylated Synthetic Oligonucleotide R&D Systems®, PN 358‐MT‐100/CF Buffered SOMAmer reagent delivered at 10 μM in 5 mM HEPES, 1 mM EDTA, pH 8 ACTIVITY SOMAmer reagents have been qualified for target affinity capture in conjunction with numerous downstream applications. Specific protocols should be determined for the intended use. General application notes are available on our website (http://www.somalogic.com/resources/app-notes/). Specificity No binding was observed with MSP R. Affinity The Kd for Hepatocyte growth factor receptor binding to the SOMAmer -11
reagent, measured by Alpha‐SA/HIS method, is typically 9 x 10 M. See
Certificate of Analysis for details. HANDLING and STORAGE
Handling Shipping Stability & Storage Heat/cool treatment on material ready for use (not stocks) is recommended (e.g. heat the reagent to 95 °C for 5 minutes, then cool to assay temperature). The product is shipped frozen. Upon receipt, store it immediately at the temperature recommended below. For long term use, aliquotting is recommended to avoid freeze‐thaw cycles.  2 years from date of receipt at ‐20 °C to ‐70 °C, as supplied
 3 months at 4 °C
TARGET FUNCTION Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3‐kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS‐ERK, PI3 kinase‐AKT, or PLCgamma‐PKC. The RAS‐ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. Acts as a receptor for Listeria internalin inlB, mediating entry of the pathogen into cells. (Source: The UniProt Consortium, Activities at the Universal Protein Resource (UniProt), Nucleic Acids Res. 42: D191‐D198 (2014).) SomaLogic, Inc. • 2945 Wilderness Place Boulder, CO 80301 • www.somalogic.com Technical Support 800‐324‐0783 • Main Number 303‐625‐9000 570‐00006, Rev 0, DCN 15‐254
Effective Date: 11/2/2015  2015 SomaLogic, Inc.