Download SOMAmer® anti-Eukaryotic translation initiation factor

SOMAmer® anti-Eukaryotic translation initiation
factor 4 gamma 2 Reagent
DESCRIPTION
SOMAmer Reagent No.
Protein Target
Target UniProt ID
Species Reactivity
5’ Functional Mod.
Target Source
Formulation
B-4230-1_2
Eukaryotic translation initiation factor 4 gamma 2
P78344
Human
Biotinylated Synthetic Oligonucleotide
Amino acids 600 to 844 with a poly-His tag
Buffered SOMAmer reagent delivered at 10 μM in 5 mM HEPES, 1 mM EDTA,
pH 8
ACTIVITY
SOMAmer reagents have been qualified for target affinity capture in conjunction with numerous
downstream applications. Specific protocols should be determined for the intended use. General
application notes are available on our website (http://www.somalogic.com/resources/app-notes/).
Specificity
No closely related human proteins were available for specificity testing.
Affinity
The Kd for Eukaryotic translation initiation factor 4 gamma 2 binding to the
-9
SOMAmer reagent, measured by Zorbax Affinity method, is typically 6 x 10 M.
See Certificate of Analysis for details.
HANDLING and STORAGE
Handling
Shipping
Stability & Storage
Heat/cool treatment on material ready for use (not stocks) is recommended
(e.g. heat the reagent to 95 °C for 5 minutes, then cool to assay temperature).
The product is shipped frozen. Upon receipt, store it immediately at the
temperature recommended below.
For long term use, aliquotting is recommended to avoid freeze-thaw cycles.
 2 years from date of receipt at -20 °C to -70 °C, as supplied
 3 months at 4 °C
TARGET FUNCTION
Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis,
apoptosis and viral infection. Cleaved by some caspases and viral proteases. (Source: The UniProt
Consortium, Activities at the Universal Protein Resource (UniProt), Nucleic Acids Res. 42: D191-D198
(2014).)
SomaLogic, Inc. • 2945 Wilderness Place Boulder, CO 80301 • www.somalogic.com
Technical Support 800-324-0783 • Main Number 303-625-9000
570-00006, Rev 0, DCN 15-326
Effective Date: 10/5/2015
 2015 SomaLogic, Inc.