Late Transition Metal Amido Complexes: Electronic
... atom.[2b] This development has led to a broad variety of early transition metal complexes with unprecedented reactivity patterns. In contrast, electron rich late transition complexes in low oxidation states (d6 - d10) with amido ligands were until more recently comparatively rare.[3] Their participa ...
... atom.[2b] This development has led to a broad variety of early transition metal complexes with unprecedented reactivity patterns. In contrast, electron rich late transition complexes in low oxidation states (d6 - d10) with amido ligands were until more recently comparatively rare.[3] Their participa ...
Joanna Kulesza
... Je tiens à exprimer ici la mémoire de mes anciennes amies: Anna Bresińska, Dominika Kozłowska, Anna Budzisz et Justyna Piotrowska. ...
... Je tiens à exprimer ici la mémoire de mes anciennes amies: Anna Bresińska, Dominika Kozłowska, Anna Budzisz et Justyna Piotrowska. ...
Biological effects of Extracellular fibrinogen binding protein (Efb) in
... produces a diverse array of virulence factors, among these Extracellular fibrinogen binding protein, Efb. Efb is one of several fibrinogen-binding proteins produced by S. aureus. Efb is produced in vivo during infection and contributes to the pathogenesis of severe S. aureus wound infection in an an ...
... produces a diverse array of virulence factors, among these Extracellular fibrinogen binding protein, Efb. Efb is one of several fibrinogen-binding proteins produced by S. aureus. Efb is produced in vivo during infection and contributes to the pathogenesis of severe S. aureus wound infection in an an ...
two types of titin interactions lead to an asymmetrical sorting of actinin
... binding site of α-actinin as an interaction between the spectrin-like repeats (slrs) and a single site on titin. We also demonstrate that the central Z-repeats of titin can interact equally with the C-terminal domain of α-actinin, similarly to the flanking repeats. These interactions control the ass ...
... binding site of α-actinin as an interaction between the spectrin-like repeats (slrs) and a single site on titin. We also demonstrate that the central Z-repeats of titin can interact equally with the C-terminal domain of α-actinin, similarly to the flanking repeats. These interactions control the ass ...
The Impact of Ligand Design on the Coordination Chemistry and
... Figure 4.2. (a) Structure of fac-ReBr(CO)3[H(LMe)], 1Me (b) Structure of the cation in {fac-Re(CO)3[H(LMe)]}(PF6), 2Me (c) Structure of fac-e(CO)3(LMe),3Me....................87 Figure 4.3.(a) Structure of H(LMe) (b) Structure of fac-ReBr(CO)3[H(LiPr)], 1iPr (c) Structure of cation in {fac-Re(CO)3[H ...
... Figure 4.2. (a) Structure of fac-ReBr(CO)3[H(LMe)], 1Me (b) Structure of the cation in {fac-Re(CO)3[H(LMe)]}(PF6), 2Me (c) Structure of fac-e(CO)3(LMe),3Me....................87 Figure 4.3.(a) Structure of H(LMe) (b) Structure of fac-ReBr(CO)3[H(LiPr)], 1iPr (c) Structure of cation in {fac-Re(CO)3[H ...
coordination of some monodentate and hybrid multident ate
... A range of low-valent late transition-metal triarylphosphine complexes have been prepared and characterised by a combination of *H, [P and 19F (as appropriate) NMR and IR spectroscopies and mass spectrometry. Some of these complexes have been isolated as single crystals and characterised by X-ray di ...
... A range of low-valent late transition-metal triarylphosphine complexes have been prepared and characterised by a combination of *H, [P and 19F (as appropriate) NMR and IR spectroscopies and mass spectrometry. Some of these complexes have been isolated as single crystals and characterised by X-ray di ...
Spectroscopic Comparisons of the pH Dependencies of Fe
... bound in the (Mn)SOD protein, its E°′ is depressed to a value well below the native E°′ of 0.2-0.4 V (19), and similarly that the E°′ of Mn bound in (Fe)SOD is insufficiently depressed, to a value well above 0.2-0.4 V (16, 17). This model is consistent with the increase in activity observed at low p ...
... bound in the (Mn)SOD protein, its E°′ is depressed to a value well below the native E°′ of 0.2-0.4 V (19), and similarly that the E°′ of Mn bound in (Fe)SOD is insufficiently depressed, to a value well above 0.2-0.4 V (16, 17). This model is consistent with the increase in activity observed at low p ...
PDF File
... suspected, mutation of G to C, or C to G, is predicted to be detrimental, whereas the double mutation, G•C to C•G, is predicted to restore function (or structure). As is generally the case, no strong conclusion can be made from the disruption alone –there are many possible explanations for the loss ...
... suspected, mutation of G to C, or C to G, is predicted to be detrimental, whereas the double mutation, G•C to C•G, is predicted to restore function (or structure). As is generally the case, no strong conclusion can be made from the disruption alone –there are many possible explanations for the loss ...
ELUCIDATING THE ROLE OF SERUM IgA
... The role of natural antibodies, particularly nIgG and nIgA, in immunity had remained enigmatic over five decades since its discovery. Natural antibodies are constitutively produced throughout the human lifespan but are poly-reactive and do not have high specificity for their targets (Boyden, 1966; M ...
... The role of natural antibodies, particularly nIgG and nIgA, in immunity had remained enigmatic over five decades since its discovery. Natural antibodies are constitutively produced throughout the human lifespan but are poly-reactive and do not have high specificity for their targets (Boyden, 1966; M ...
Isotope-Exchange Evidence that Glucose 6
... ‘lower’ levels of the Scheme. As B occurs twice in the pathway between Q and A we should expect from consideration of the method of King and Altman [I31 that the expression for F(Q -+ A) would contain terms in [BIZ,and detailed algebraic analysis confirms this and shows that a plot of the flux ratio ...
... ‘lower’ levels of the Scheme. As B occurs twice in the pathway between Q and A we should expect from consideration of the method of King and Altman [I31 that the expression for F(Q -+ A) would contain terms in [BIZ,and detailed algebraic analysis confirms this and shows that a plot of the flux ratio ...
Structural Analyses of Zinc Finger Domains for Specific Interactions
... they provide critical roles in physiological systems from prokaryotes to eukaryotes. Two cysteine and two histidine residues (Cys2His2) coordinate to the zinc ion for the structural functions to generate a ββα fold, and this secondary structure supports specific interactions with their binding partn ...
... they provide critical roles in physiological systems from prokaryotes to eukaryotes. Two cysteine and two histidine residues (Cys2His2) coordinate to the zinc ion for the structural functions to generate a ββα fold, and this secondary structure supports specific interactions with their binding partn ...
estrogen receptor by cyclin D1 Ligand
... D1 mutants (2.5 µg), an internal control -galactosidase plasmid (0.5 µg), and an ERE–TATA–luciferase reporter (3 µg). The effect on ER transactivation of wild-type cyclin D1 was set to 100%. These studies were performed in three separate experiments and expressed as mean values with S.D. < 10 % (da ...
... D1 mutants (2.5 µg), an internal control -galactosidase plasmid (0.5 µg), and an ERE–TATA–luciferase reporter (3 µg). The effect on ER transactivation of wild-type cyclin D1 was set to 100%. These studies were performed in three separate experiments and expressed as mean values with S.D. < 10 % (da ...
Mycobacteria Target DC-SIGN to Suppress Dendritic Cell Function
... HIV-1 gp120 (19), as a specific mutation in DC-SIGN (V351G) abrogated ICAM-3, but not HIV-1 gp120 binding (Fig. 2 c, and reference 19). Strikingly, the DC-SIGN V351G mutant also interacts with M. bovis BCG as well as ManLAM (Fig. 2 c), demonstrating that both HIV-1 and mycobacteria bind similarly to ...
... HIV-1 gp120 (19), as a specific mutation in DC-SIGN (V351G) abrogated ICAM-3, but not HIV-1 gp120 binding (Fig. 2 c, and reference 19). Strikingly, the DC-SIGN V351G mutant also interacts with M. bovis BCG as well as ManLAM (Fig. 2 c), demonstrating that both HIV-1 and mycobacteria bind similarly to ...
Investigations of non-enzymatic glycation with new bioanalytical
... Of all the modified sites (59) on the Bβ-chain, 25 were found by analysing the digest, while this number increased with fractionations on C30-silica (53 sites) and C60(30) (47 sites). Forty-one possible glycated sites were found for the c-chain of fibrinogen. Of these, 24 were recognised from the d ...
... Of all the modified sites (59) on the Bβ-chain, 25 were found by analysing the digest, while this number increased with fractionations on C30-silica (53 sites) and C60(30) (47 sites). Forty-one possible glycated sites were found for the c-chain of fibrinogen. Of these, 24 were recognised from the d ...
Monoclonal Antibody between Cockroach Allergen Bla g 2 and a
... 1.8 Å resolution. Binding of 4C3 involves different types of molecular interactions with its epitope compared with those with the mAb 7C11, which binds to the N-terminal lobe of Bla g 2. We found that the 4C3 surface epitope on Bla g 2 includes a carbohydrate moiety attached to Asn268 and that a la ...
... 1.8 Å resolution. Binding of 4C3 involves different types of molecular interactions with its epitope compared with those with the mAb 7C11, which binds to the N-terminal lobe of Bla g 2. We found that the 4C3 surface epitope on Bla g 2 includes a carbohydrate moiety attached to Asn268 and that a la ...
Identification of core functional region of murine IL-4 using
... Experiments were repeated at least twice, and usually three times. Figures show data compiled from several experiments, or from a representative experiment, as specified. Results represent the mean 6 SD, where applicable. Statistical significance of differences was analyzed using the independent Stu ...
... Experiments were repeated at least twice, and usually three times. Figures show data compiled from several experiments, or from a representative experiment, as specified. Results represent the mean 6 SD, where applicable. Statistical significance of differences was analyzed using the independent Stu ...
1337 Biochemical properties of plasminogen activator inhibitor
... reversible, non-covalent Michaelis complexes formed between a serpin and protease, in which the active site serine had been replaced by an alanine: Manduca sexta serpin 1B A353K with rat trypsin (50); alpha1-proteinase inhibitor Pittsburgh with bovine trypsin (51); heparin cofactor II with thrombin ...
... reversible, non-covalent Michaelis complexes formed between a serpin and protease, in which the active site serine had been replaced by an alanine: Manduca sexta serpin 1B A353K with rat trypsin (50); alpha1-proteinase inhibitor Pittsburgh with bovine trypsin (51); heparin cofactor II with thrombin ...
Chapter One Hemilabile Ligands in Transition
... Transition metal organic and organometallic chemistry lie at the interface between classical organic and inorganic chemistry because they look at the interaction between inorganic metal ions and organic molecules. Transition metal ions act as Lewis acids and can bind to Lewis bases known as ligands ...
... Transition metal organic and organometallic chemistry lie at the interface between classical organic and inorganic chemistry because they look at the interaction between inorganic metal ions and organic molecules. Transition metal ions act as Lewis acids and can bind to Lewis bases known as ligands ...
Formation of a highly peptide-receptive state of class II MHC.
... binding over a fixed time interval (3 min, Figure 4), or by measuring the time dependence of binding of a fixed concentration of peptide (data not shown). In both cases, the results are consistent with reaction scheme (3), with a rate of peptide binding of z5 3 105 M21 s21 found for the MCC 95-103 p ...
... binding over a fixed time interval (3 min, Figure 4), or by measuring the time dependence of binding of a fixed concentration of peptide (data not shown). In both cases, the results are consistent with reaction scheme (3), with a rate of peptide binding of z5 3 105 M21 s21 found for the MCC 95-103 p ...
HYBRID MULTIDENTATE PHOSPHINE
... cycloisomerisation reactions. In addition to the coordination chemistry of the novel ligand systems, some interesting findings emerged For example, AuI complex of Lei ligand 17 and monodbaPHOS 74 undergoes an interesting solid-state [2+2] intramolecular cycloaddition transformation, giving cycloaddu ...
... cycloisomerisation reactions. In addition to the coordination chemistry of the novel ligand systems, some interesting findings emerged For example, AuI complex of Lei ligand 17 and monodbaPHOS 74 undergoes an interesting solid-state [2+2] intramolecular cycloaddition transformation, giving cycloaddu ...
Cohesin: A Multi-purpose Chromatin Glue
... Although CTCF binding to DNA is sequence specific, there are an estimated 14 000 – 20 000 CTCF binding sites in the human genome, which happen to lie in gene-rich regions, hinting at an important role of CTCF in the regulation of gene expression. More importantly, CTCF has been shown to be involved ...
... Although CTCF binding to DNA is sequence specific, there are an estimated 14 000 – 20 000 CTCF binding sites in the human genome, which happen to lie in gene-rich regions, hinting at an important role of CTCF in the regulation of gene expression. More importantly, CTCF has been shown to be involved ...
The life of ribulose 1,5-bisphosphate carboxylase/oxygenase
... manner with RuBP at the active site [79]. This model can account for most ‘‘positive’’ and ‘‘negative’’ effectors and order of addition and dilution effects with in vitro assays reported in the early literature. Under steady state conditions with this model, all effectors will increase the Km for RuBP ...
... manner with RuBP at the active site [79]. This model can account for most ‘‘positive’’ and ‘‘negative’’ effectors and order of addition and dilution effects with in vitro assays reported in the early literature. Under steady state conditions with this model, all effectors will increase the Km for RuBP ...
Mutations of the PC2 Substrate Binding Pocket Alter Enzyme
... of a prodomain, believed to assist in correct folding of the enzyme (2– 4), a catalytic domain with some homology to bacterial subtilisin, and a P- or homo-B domain that plays an essential role in regulating stability and modulating activity (4). The proprotein convertases are thought to be responsi ...
... of a prodomain, believed to assist in correct folding of the enzyme (2– 4), a catalytic domain with some homology to bacterial subtilisin, and a P- or homo-B domain that plays an essential role in regulating stability and modulating activity (4). The proprotein convertases are thought to be responsi ...
Mapping the Heparin-binding Sites on Type I Collagen Monomers
... migration patterns within ACE gels were obtained using a phosphorimager (Molecular Dynamics). From these electrophoretograms, the dissociation constant (Kd) Can be estimated from the protein concentration at which the heparin is half-shifted from being fully mobile at very low protein concentrations ...
... migration patterns within ACE gels were obtained using a phosphorimager (Molecular Dynamics). From these electrophoretograms, the dissociation constant (Kd) Can be estimated from the protein concentration at which the heparin is half-shifted from being fully mobile at very low protein concentrations ...
Sampson, Probing the Specifics of Substrate Binding for
... present in COX and the exceedingly complex nature of the process, it was not practical to use the entire enzyme for the minimization study: Instead, as done by Roberts and Pique (23), we used only a fraction of the enzyme. A region of ~25 Å in radius which encompassed all of the amino acid residues ...
... present in COX and the exceedingly complex nature of the process, it was not practical to use the entire enzyme for the minimization study: Instead, as done by Roberts and Pique (23), we used only a fraction of the enzyme. A region of ~25 Å in radius which encompassed all of the amino acid residues ...
Cooperative binding
Molecular binding is an interaction between molecules that results in a stable physical association between those molecules. Cooperative binding occurs in binding systems that are constituted by more than one type (species) of molecule (say molecules A and B) and in which one of the partners is not mono-valent; i.e., it binds more than one molecule of the other molecular species. For example, one molecule of type A can bind 6 molecules of type B (in such cases, B is usually referred to as the ""ligand""). Binding in this type of system can be considered ""cooperative"" if the binding of B to one site on A is affected by the binding of B to other site(s) on A. In other words, the binding of B molecules to the different sites on A do not constitute mutually independent events. This can be due, for instance, to an affinity for the ligand that depends on the amount of ligand bound. Cooperativity can be positive or negative. Cooperative binding is observed in many biopolymers, including proteins and nucleic acids. Cooperative binding has been shown to be the mechanism underlying a large range of biochemical and physiological processes.