Modulation of the Antigenic Peptide Transporter TAP by
... fragments was analyzed by ELISA using the NBD of TAP1 as antigen.29 The equilibrium binding constants (KD) for the monovalent recombinant Fv (31.8( ± 2.7) nM) and the Fab fragment (39.5( ± 2.7) nM) are very similar (Figure 6(a) and (b)), however, they differed drastically from the KD value of the mA ...
... fragments was analyzed by ELISA using the NBD of TAP1 as antigen.29 The equilibrium binding constants (KD) for the monovalent recombinant Fv (31.8( ± 2.7) nM) and the Fab fragment (39.5( ± 2.7) nM) are very similar (Figure 6(a) and (b)), however, they differed drastically from the KD value of the mA ...
2015_GRC-muscle_abstract_mto_150515
... We recently identified the first two human missense mutations in a calmodulin (CaM) gene (CALM1) and linked these to catecholaminergic polymorphic ventricular tachycardia (CPVT) and sudden cardiac death in young individuals. More CaM mutations have since been identified in CALM1 and also in the othe ...
... We recently identified the first two human missense mutations in a calmodulin (CaM) gene (CALM1) and linked these to catecholaminergic polymorphic ventricular tachycardia (CPVT) and sudden cardiac death in young individuals. More CaM mutations have since been identified in CALM1 and also in the othe ...
Carboxypeptidase A - Chemistry Courses: About
... was questioned in Kaiser’s laboratory by the results of a parallel resonance Raman cryospectroscopic Later, Suh and colleagues demonstrated the accumulation of an intermediate, presumed to be the acyl enzyme, at -2 OC in the hydrolysis of different ester substrates.% The designation of acyl enzyme w ...
... was questioned in Kaiser’s laboratory by the results of a parallel resonance Raman cryospectroscopic Later, Suh and colleagues demonstrated the accumulation of an intermediate, presumed to be the acyl enzyme, at -2 OC in the hydrolysis of different ester substrates.% The designation of acyl enzyme w ...
Increased Agonist Affinity at the
... M1 A488 intensity was measured to give an R/G value. The magnitude of the R/G values is dependent upon efficiency of antibody–fluorophore conjugation, exposure time, laser intensity, and efficiency of light collection for each wavelength and do not represent an absolute ratio of ligand bound per rec ...
... M1 A488 intensity was measured to give an R/G value. The magnitude of the R/G values is dependent upon efficiency of antibody–fluorophore conjugation, exposure time, laser intensity, and efficiency of light collection for each wavelength and do not represent an absolute ratio of ligand bound per rec ...
Catalytic and transport cycles of ABC exporters
... ers, other topologies ranging from five to ten α‑helices per TMD have been observed [13,14]. Thus the TMDs of importers and exporters cannot be easily interchanged in models of transport. It is best to consider the coupling mechanisms of these proteins in three distinct groups on the basis of their ...
... ers, other topologies ranging from five to ten α‑helices per TMD have been observed [13,14]. Thus the TMDs of importers and exporters cannot be easily interchanged in models of transport. It is best to consider the coupling mechanisms of these proteins in three distinct groups on the basis of their ...
Determination of Binding Sites of Cadherin Peptides on the EC1
... The binding between the complex of natural killer cell receptor KLRG1 and the EC1 domain of E-cadherin has been determined using X-ray crystallography and residue mutation experiments [21]. Solution NMR and X-ray crystallography of ECAD12 were studied to evaluate the binding activity of the N-termin ...
... The binding between the complex of natural killer cell receptor KLRG1 and the EC1 domain of E-cadherin has been determined using X-ray crystallography and residue mutation experiments [21]. Solution NMR and X-ray crystallography of ECAD12 were studied to evaluate the binding activity of the N-termin ...
lecture notes-enzyme-web
... Models for More Complex Enzyme Kinetics • Allosteric enzymes - Some enzymes have more than one substrate binding site. - Allostery or cooperative binding: the binding of one substrate to the enzyme facilitates binding of other substrate molecules. ...
... Models for More Complex Enzyme Kinetics • Allosteric enzymes - Some enzymes have more than one substrate binding site. - Allostery or cooperative binding: the binding of one substrate to the enzyme facilitates binding of other substrate molecules. ...
PAI-1 - Trace: Tennessee Research and Creative Exchange
... and there is positive correlation between high levels of PAI-1 and invasiveness of certain types of cancer.1 The PAI-1 serpin is comprised of 379 amino acids to produce a molecular weight of 43 kilodaltons (kDa) in its recombinant form, or approximately 50 kDa in its glycosylated native form. This p ...
... and there is positive correlation between high levels of PAI-1 and invasiveness of certain types of cancer.1 The PAI-1 serpin is comprised of 379 amino acids to produce a molecular weight of 43 kilodaltons (kDa) in its recombinant form, or approximately 50 kDa in its glycosylated native form. This p ...
PDF File
... the observed K1/2 values equal the dissociation constants Kd (see also ref 37): The same K1/2 values were observed in concentration dependences in which the maximal rate constant for reaction varied by more than 10-fold, which was accomplished by a 2′-H substitution at position -1 and by varying the ...
... the observed K1/2 values equal the dissociation constants Kd (see also ref 37): The same K1/2 values were observed in concentration dependences in which the maximal rate constant for reaction varied by more than 10-fold, which was accomplished by a 2′-H substitution at position -1 and by varying the ...
Published version
... adsorbed onto 96-well plates. Bound protein was detected with antibodies and measured as absorbance at 450 nm. Red, collagen type I; green, VCL-Int; cyan, G508S; purple, G505S; orange, G(⫺9)S; blue, G(⫺15)S. Data were fit in GraphPad Prism姞 using a non-linear fit (one site, specific binding). Error ...
... adsorbed onto 96-well plates. Bound protein was detected with antibodies and measured as absorbance at 450 nm. Red, collagen type I; green, VCL-Int; cyan, G508S; purple, G505S; orange, G(⫺9)S; blue, G(⫺15)S. Data were fit in GraphPad Prism姞 using a non-linear fit (one site, specific binding). Error ...
Enzymes: Catalytic Strategies
... • 1st 4 mechanisms related to BINDING of substrate and/or transition state state, (reaction takes place in active site, not in bulk solution) 1. PROXIMITY AND ORIENTATION (catalysis by “approximation”) – Proximity • Reaction between bound molecules doesn't require an improbable collision of 2 molecu ...
... • 1st 4 mechanisms related to BINDING of substrate and/or transition state state, (reaction takes place in active site, not in bulk solution) 1. PROXIMITY AND ORIENTATION (catalysis by “approximation”) – Proximity • Reaction between bound molecules doesn't require an improbable collision of 2 molecu ...
Supplementary Figure 2. Further examples of large differences
... true negative residues (TNs) the MCC score is further increased to 0.705, however the BDT score remains at 0.333. B) Ribbon diagram of CASP8 target T0426. This example shows a hypothetical prediction highlighting the difference between the MCC and BDT scores for the predicted residues close to the b ...
... true negative residues (TNs) the MCC score is further increased to 0.705, however the BDT score remains at 0.333. B) Ribbon diagram of CASP8 target T0426. This example shows a hypothetical prediction highlighting the difference between the MCC and BDT scores for the predicted residues close to the b ...
Reversible binding of sulfur dioxide to arylplatinum (II) and nickel (II
... geometry with two trans N atoms, a Br atom, and a C atom in the base and the S atom of the &bonded SOz ligand at the apex (long Pt-S bond of 2.613 ( 7 ) A) (see Figure 4a). The binding of the SOz to Pt(I1) and Ni(I1) centers points to an enhanced basicity of these metals. This arises from the presen ...
... geometry with two trans N atoms, a Br atom, and a C atom in the base and the S atom of the &bonded SOz ligand at the apex (long Pt-S bond of 2.613 ( 7 ) A) (see Figure 4a). The binding of the SOz to Pt(I1) and Ni(I1) centers points to an enhanced basicity of these metals. This arises from the presen ...
Print this article - Bangladesh Journals Online
... assignable for protons Hd and Ha respectively. The two doublets of doublet at δ 6.5 (JHa-Hb = JHb-Hc = J = 8.0 Hz) and 6.9 (JHb-Hc= JHc-Hd = J = 8.0 Hz) accounts for the Ha and Hd respectively, while the relatively downfield signal at δ 8.5 has been assigned for the imine (=N-H) proton of 2-mercapto ...
... assignable for protons Hd and Ha respectively. The two doublets of doublet at δ 6.5 (JHa-Hb = JHb-Hc = J = 8.0 Hz) and 6.9 (JHb-Hc= JHc-Hd = J = 8.0 Hz) accounts for the Ha and Hd respectively, while the relatively downfield signal at δ 8.5 has been assigned for the imine (=N-H) proton of 2-mercapto ...
Microsoft Word
... derived by homology modelling were very similar to that of the eIF4E template due to high sequence homology (Fig 1.). Therefore, the structural differences of potential importance for the MMG- vs. TMG-cap binding selectivity have not been identified. This prompted us to evaluate a discrimination mec ...
... derived by homology modelling were very similar to that of the eIF4E template due to high sequence homology (Fig 1.). Therefore, the structural differences of potential importance for the MMG- vs. TMG-cap binding selectivity have not been identified. This prompted us to evaluate a discrimination mec ...
Sign of enthalpy changes Exothermic vs endothermic Acid
... Problem: 0.3 mL of 1mM solution of a drug is added to a tube with a protein solution. Some of the drug reacts with the protein to form 1:1 protein-drug complex. The reaction produces a heat of –2.4 x 10–3 cal. Estimate the molar enthalpy of binding binding. ...
... Problem: 0.3 mL of 1mM solution of a drug is added to a tube with a protein solution. Some of the drug reacts with the protein to form 1:1 protein-drug complex. The reaction produces a heat of –2.4 x 10–3 cal. Estimate the molar enthalpy of binding binding. ...
Ribosomal crystallography: a flexible nucleotide anchoring tRNA
... I. Agmon et al. / FEBS Letters 567 (2004) 20–26 ...
... I. Agmon et al. / FEBS Letters 567 (2004) 20–26 ...
AA-tRNA - Studentportalen
... •EF-Tu*GTP*AA-tRNA complex binds to the empty Asite if the tRNA anticodon matches the codon in the Asite. This is checked by the 30S ribosomal subunit. If the match is good the hydrolysis of GTP on EF-Tu occurs and EF-Tu looses its affinity to the CCA end of tRNA. This CCA end carries an aminoacid a ...
... •EF-Tu*GTP*AA-tRNA complex binds to the empty Asite if the tRNA anticodon matches the codon in the Asite. This is checked by the 30S ribosomal subunit. If the match is good the hydrolysis of GTP on EF-Tu occurs and EF-Tu looses its affinity to the CCA end of tRNA. This CCA end carries an aminoacid a ...
Enzymes: Biological Catalysts
... Thus, the equilibrium constant K tells nothing about the rates of process. K depends only on the free energy difference between final and initial states and carries no information about the height of the barrier between these states. Summary of important points: 1. Reactions should go faster at high ...
... Thus, the equilibrium constant K tells nothing about the rates of process. K depends only on the free energy difference between final and initial states and carries no information about the height of the barrier between these states. Summary of important points: 1. Reactions should go faster at high ...
Phages from landscape libraries as substitute antibodies
... also inhibits binding, although with 10 times lower affinity than native and denatured phages (data not shown). Binding of the synthetic peptide to streptavidin could also be demonstrated by direct ELISA (data not shown). Taken together, these results show that the phage body serves only as a carrie ...
... also inhibits binding, although with 10 times lower affinity than native and denatured phages (data not shown). Binding of the synthetic peptide to streptavidin could also be demonstrated by direct ELISA (data not shown). Taken together, these results show that the phage body serves only as a carrie ...
Chimie de l`H érédité.
... Two important facts must always be considered when thinking about ribosomes. The first is that they are chemically very complex. The second is that they are always constructed from two dissociable subunits, one approximately twice the size of the other. Over 30 different structura ...
... Two important facts must always be considered when thinking about ribosomes. The first is that they are chemically very complex. The second is that they are always constructed from two dissociable subunits, one approximately twice the size of the other. Over 30 different structura ...
Role of Cys-295 on subunit interactions and allosteric regulation of
... protein concentration has been held constant. The scattered intensity correlated well with the loss of enzymatic activity, dropping to less than half of the initial value at the same GdnHCl concentration range where loss of activity was observed. Since unfolding proceeds from the native dimeric stat ...
... protein concentration has been held constant. The scattered intensity correlated well with the loss of enzymatic activity, dropping to less than half of the initial value at the same GdnHCl concentration range where loss of activity was observed. Since unfolding proceeds from the native dimeric stat ...
Structure of Mammalian AMPK and its regulation by ADP
... Notably, their studies revealed that three of the four potential nucleotide-binding sites are occupied. One of these sites contains a permanently bound AMP molecule (site 4, ) whereas AMP and Mg-ATP compete for binding at the other two sites (site 1 and site 3). ...
... Notably, their studies revealed that three of the four potential nucleotide-binding sites are occupied. One of these sites contains a permanently bound AMP molecule (site 4, ) whereas AMP and Mg-ATP compete for binding at the other two sites (site 1 and site 3). ...
Lecture V. Spatial organization in cells and embryos
... bind to the receptor. So to the model of Barkai we have to add another feature: Tsg mediates Dpp binding to the receptor. Scw and Dpp are not independent. This is not just another seeming redundant or partially redundant pathway. Dpp binding requires that Scw be present at the same place. Finally th ...
... bind to the receptor. So to the model of Barkai we have to add another feature: Tsg mediates Dpp binding to the receptor. Scw and Dpp are not independent. This is not just another seeming redundant or partially redundant pathway. Dpp binding requires that Scw be present at the same place. Finally th ...
Cooperative binding
Molecular binding is an interaction between molecules that results in a stable physical association between those molecules. Cooperative binding occurs in binding systems that are constituted by more than one type (species) of molecule (say molecules A and B) and in which one of the partners is not mono-valent; i.e., it binds more than one molecule of the other molecular species. For example, one molecule of type A can bind 6 molecules of type B (in such cases, B is usually referred to as the ""ligand""). Binding in this type of system can be considered ""cooperative"" if the binding of B to one site on A is affected by the binding of B to other site(s) on A. In other words, the binding of B molecules to the different sites on A do not constitute mutually independent events. This can be due, for instance, to an affinity for the ligand that depends on the amount of ligand bound. Cooperativity can be positive or negative. Cooperative binding is observed in many biopolymers, including proteins and nucleic acids. Cooperative binding has been shown to be the mechanism underlying a large range of biochemical and physiological processes.