First-Principles Theoretical Study of Molecular HCl Adsorption on a
... temperatures is developed, in section V. Atmospheric implications are briefly discussed in section VI. Concluding remarks are made in section VII. II. Ice Surface Models As a first attempt to model adsorption of molecular HCl on a hexagonal ice basal (0001) face,53 which may be the most prevalent un ...
... temperatures is developed, in section V. Atmospheric implications are briefly discussed in section VI. Concluding remarks are made in section VII. II. Ice Surface Models As a first attempt to model adsorption of molecular HCl on a hexagonal ice basal (0001) face,53 which may be the most prevalent un ...
Cyclam ``capa` POT.4` to ``capa` POT.3` denticity change
... linkers because they can form amide bonds with a desired material or relevant biomolecules such as proteins or antibodies. Whereas complex-modified solid materials may lead, for example, to sensors with potential analytical applications, the attachment of such compounds to biomolecules may form NO c ...
... linkers because they can form amide bonds with a desired material or relevant biomolecules such as proteins or antibodies. Whereas complex-modified solid materials may lead, for example, to sensors with potential analytical applications, the attachment of such compounds to biomolecules may form NO c ...
Identification and Structural Characterization of the ATP/ADP
... Hsp70/DnaK. Although the apparent involvement of ATP in p23 binding remains unexplained, it has become widely accepted that unlike Hsp70/DnaK and Hsp60/ GroEL, Hsp90 does not bind ATP. Although the structure of the intact Hsp90 molecule has not yet been determined, crystal structures of an amino-ter ...
... Hsp70/DnaK. Although the apparent involvement of ATP in p23 binding remains unexplained, it has become widely accepted that unlike Hsp70/DnaK and Hsp60/ GroEL, Hsp90 does not bind ATP. Although the structure of the intact Hsp90 molecule has not yet been determined, crystal structures of an amino-ter ...
... hemin under the standard conditions. The dialcohol (8) was incubated with peroxidase in 5 mM potassium acetate, pH 4.4, containing 10% by volume acetone, again for solubility reasons. Reconstitution of peroxidase with protohemin-free acid in the Tris-pyridine buffer system gave a highly active enzym ...
Glucansucrases: mechanism of action and structure–function
... Glucansucrases catalyse transfer of glucosyl residues coming from sucrose cleavage. The synthesis of di¡erent products by glucansucrases depends on the destination of these glucosyl units [37] (Fig. 1). The dextran synthesis reaction occurs by successive transfer of glucosyl units to the polymer. In ...
... Glucansucrases catalyse transfer of glucosyl residues coming from sucrose cleavage. The synthesis of di¡erent products by glucansucrases depends on the destination of these glucosyl units [37] (Fig. 1). The dextran synthesis reaction occurs by successive transfer of glucosyl units to the polymer. In ...
in search of intramolecular chaperone
... effect on the final structure of a protein. In contrast, the chainlinked molecular chaperones have been proposed to act by conveying steric information that is essential for correct folding (Shinde et al., 1997). However, subsequently, the fragment playing this role is cleaved off. Hence, in this se ...
... effect on the final structure of a protein. In contrast, the chainlinked molecular chaperones have been proposed to act by conveying steric information that is essential for correct folding (Shinde et al., 1997). However, subsequently, the fragment playing this role is cleaved off. Hence, in this se ...
Binding of Thrombin to Subendothelial Extracellular Matrix
... Traditionally, the response to vascular injury is considered to begin when rapid activation of the hemostatic process is initiated after exposure of the subendothelium. This would then lead to thrombin generation, platelet activation and fibrin clot formation to establish a hemostatic plug (6). It h ...
... Traditionally, the response to vascular injury is considered to begin when rapid activation of the hemostatic process is initiated after exposure of the subendothelium. This would then lead to thrombin generation, platelet activation and fibrin clot formation to establish a hemostatic plug (6). It h ...
Myosin V Plays an Essential Role in the Thyroid Hormone
... microfilaments and activates actin-based endocytosis of D2containing vesicles, leading to a rapid fall in D2 levels in the cell activity (5, 8, 9). Importantly, repolymerization of the microfilaments in the absence of thyroid hormone does not alter D2 turnover or activate the actin-based endocytosis ...
... microfilaments and activates actin-based endocytosis of D2containing vesicles, leading to a rapid fall in D2 levels in the cell activity (5, 8, 9). Importantly, repolymerization of the microfilaments in the absence of thyroid hormone does not alter D2 turnover or activate the actin-based endocytosis ...
13- and 14-membered macrocyclic ligands containing
... and teta) or methylphosphonate (tritp and tetp) arms were determined. All the ligands were labelled with 153Sm and 166Ho in order to evaluate the effect of the macrocyclic cavity size and type of appended arms on their in vitro and in vivo behaviour. The radiolabelling efficiency was found to be higher ...
... and teta) or methylphosphonate (tritp and tetp) arms were determined. All the ligands were labelled with 153Sm and 166Ho in order to evaluate the effect of the macrocyclic cavity size and type of appended arms on their in vitro and in vivo behaviour. The radiolabelling efficiency was found to be higher ...
Session 1B - Rubisco and Photorespiration
... substrate Ribulose 1, 5-bisphosphate (RuBP) and other sugar phosphates. The AAA+ protein Rubisco activase (Rca) counteracts this issue by removing these inhibitors and thus maintaining Rubisco in its active state. Inactivation of Rca at moderately high temperatures is associated with the loss of the ...
... substrate Ribulose 1, 5-bisphosphate (RuBP) and other sugar phosphates. The AAA+ protein Rubisco activase (Rca) counteracts this issue by removing these inhibitors and thus maintaining Rubisco in its active state. Inactivation of Rca at moderately high temperatures is associated with the loss of the ...
Spectral characterization of the binding and conformational changes
... spectrum and effectiveness. The most common parabens used in cosmetic products are methylparaben (MePa) (Fig. 2), ethylparaben (EtPa), propylparaben (PrPa) and butylparaben (BuPa). They are often used in combination, since they have synergistic effects, in a wide variety of products such as cosmetic ...
... spectrum and effectiveness. The most common parabens used in cosmetic products are methylparaben (MePa) (Fig. 2), ethylparaben (EtPa), propylparaben (PrPa) and butylparaben (BuPa). They are often used in combination, since they have synergistic effects, in a wide variety of products such as cosmetic ...
CO2 Binding and Induced Structural Collapse of a Surface
... experimental STM images and confirm the adapted coordination motifs. Dosing of 100 langmuir (L) of CO2 gas at a sample temperature of 105 K results in substantial structural changes of the network; i.e., BDBA molecules and Fe atoms form an irregular pattern. In this process, the pristine Fe dimer mot ...
... experimental STM images and confirm the adapted coordination motifs. Dosing of 100 langmuir (L) of CO2 gas at a sample temperature of 105 K results in substantial structural changes of the network; i.e., BDBA molecules and Fe atoms form an irregular pattern. In this process, the pristine Fe dimer mot ...
Classification - HAL
... CCR5 is the principal coreceptor for entry of human immunodeficiency virus type-1 (HIV-1), used together with CD4 to enter and infect target cells (1), and a receptor for agonist (CCL3/MIP-1, CCL4/MIP-1, CCL5/RANTES) and antagonist/weak partial agonist (CCL7/MCP-3) chemokines (2, 3). The native ag ...
... CCR5 is the principal coreceptor for entry of human immunodeficiency virus type-1 (HIV-1), used together with CD4 to enter and infect target cells (1), and a receptor for agonist (CCL3/MIP-1, CCL4/MIP-1, CCL5/RANTES) and antagonist/weak partial agonist (CCL7/MCP-3) chemokines (2, 3). The native ag ...
Natalia Gromak, Alexis Rideau,
... Overexpression of PTB in SM cells led to a small increase in exon 3 skipping if the long PTB4 isoform was transfected, but a decrease with the shorter PTB1 isoform (Wollerton et al., 2001). In contrast to all other known examples of PTB-regulated splicing, with TM exon 3, strong repression only occu ...
... Overexpression of PTB in SM cells led to a small increase in exon 3 skipping if the long PTB4 isoform was transfected, but a decrease with the shorter PTB1 isoform (Wollerton et al., 2001). In contrast to all other known examples of PTB-regulated splicing, with TM exon 3, strong repression only occu ...
Scalable graphene field-effect sensors for specific protein detection
... our specific microfluidic geometry [19]. At higher thrombin concentrations, an increase in concentration has less effect on the initial rate of binding. This is consistent with a two-step (diffusion–reaction) process as the binding reaction becomes rate limiting. The unbinding process can be seen wh ...
... our specific microfluidic geometry [19]. At higher thrombin concentrations, an increase in concentration has less effect on the initial rate of binding. This is consistent with a two-step (diffusion–reaction) process as the binding reaction becomes rate limiting. The unbinding process can be seen wh ...
Carbohydrate Biosensors
... reader is referred to relevant literature.1 We have also not discussed here the highly technologically and commercially important field of glucose sensing, an active area of research because of the profound health effects of aberrant glucose levels in diabetes, as glucose is technically a monosaccha ...
... reader is referred to relevant literature.1 We have also not discussed here the highly technologically and commercially important field of glucose sensing, an active area of research because of the profound health effects of aberrant glucose levels in diabetes, as glucose is technically a monosaccha ...
Distribution of Muscarinic Acetylcholine
... Electrical Recording and lontophoresis Standard electrophysiological recording and iontophoretic techniques were used as previously described (21-23). Recording microelectrodes were fabricated from fiber-filled capillaries, were backfilled with 3 M KCI, and had resistances of 60-120 MS2. lontophoret ...
... Electrical Recording and lontophoresis Standard electrophysiological recording and iontophoretic techniques were used as previously described (21-23). Recording microelectrodes were fabricated from fiber-filled capillaries, were backfilled with 3 M KCI, and had resistances of 60-120 MS2. lontophoret ...
Biochemical properties and structural features of the thermostable
... residues, which are conserved in the primary structures of all enzymes of the α-amylase family (see Fig. 2), are Glu414 (proton donor, protonates the glycosidic oxygen), Asp385 (nucleophile, for nucleophilic attack at C1), and Asp468 (catalytic base, thought to assist in attack of incoming acceptor) ...
... residues, which are conserved in the primary structures of all enzymes of the α-amylase family (see Fig. 2), are Glu414 (proton donor, protonates the glycosidic oxygen), Asp385 (nucleophile, for nucleophilic attack at C1), and Asp468 (catalytic base, thought to assist in attack of incoming acceptor) ...
Tyr130 phosphorylation triggers Syk release
... Syk’s catalytic activity (11) and generate docking sites for SH2 domain-containing proteins, such as c-Cbl, PLC␥, and Vav1. Alternative patterns of phosphorylation in interdomain B are differentially recognized, such as the preferential binding of a doubly phosphorylated region by a single SH2 of PL ...
... Syk’s catalytic activity (11) and generate docking sites for SH2 domain-containing proteins, such as c-Cbl, PLC␥, and Vav1. Alternative patterns of phosphorylation in interdomain B are differentially recognized, such as the preferential binding of a doubly phosphorylated region by a single SH2 of PL ...
FOR ENZYMES THE LIMITS FOR LIFE DEFINE THE LIMITS
... in general. Any required chemical reaction must occur faster than the lifetime of a cell. But any specific microbe cannot be too leisurely in its reproductive time, since then other species with faster rates will come to dominate the available resources. The natural driving force from competition wi ...
... in general. Any required chemical reaction must occur faster than the lifetime of a cell. But any specific microbe cannot be too leisurely in its reproductive time, since then other species with faster rates will come to dominate the available resources. The natural driving force from competition wi ...
Synthesis and thermal decarbonylation of W(CO)5 complexes
... control the nature of the metal containing particles resulting in the thermal decomposition. On the other hand, the thermograms also indicated that, in the case of 4, and 6b and, even more conspicuously, in the case of 6a, the final residues left by the complexes after an stabilization step at 800 C ...
... control the nature of the metal containing particles resulting in the thermal decomposition. On the other hand, the thermograms also indicated that, in the case of 4, and 6b and, even more conspicuously, in the case of 6a, the final residues left by the complexes after an stabilization step at 800 C ...
Supramolecular Chemistry—Scope and Perspectives Molecules
... Supramolecular chemistry is the chemistry of the intermolecular bond, covering the structures and functions of the entities formed by association of two or more chemical species. Molecular recognition in the supermolecules formed by receptor-substrate binding rests on the principles of molecular com ...
... Supramolecular chemistry is the chemistry of the intermolecular bond, covering the structures and functions of the entities formed by association of two or more chemical species. Molecular recognition in the supermolecules formed by receptor-substrate binding rests on the principles of molecular com ...
A comparison of the effects of fluoride and chloride
... YADH to contain 18-19 moles of sulfhydryl groups per mole of enzyme. Hoch and Vallee (26) arrived at a value of 25 moles of free sulfhydryl groups per molecule using the silver nitrate amperometric method. Hoch and Vallee also used PCMB and found a value of 16 free sulfhydryl groups. ...
... YADH to contain 18-19 moles of sulfhydryl groups per mole of enzyme. Hoch and Vallee (26) arrived at a value of 25 moles of free sulfhydryl groups per molecule using the silver nitrate amperometric method. Hoch and Vallee also used PCMB and found a value of 16 free sulfhydryl groups. ...
E - mustafaaltinisik.org.uk
... 1. kcat is the 1st order rate constant describing ES E+P 2. Also known as the turnover # because it describes the number of rxns a molecule of enzyme can catalyze per second under optimal condition. 3. Most enzyme have kcat values between 102 and 103 s-1 4. For simple reactions k2 = kcat , for mul ...
... 1. kcat is the 1st order rate constant describing ES E+P 2. Also known as the turnover # because it describes the number of rxns a molecule of enzyme can catalyze per second under optimal condition. 3. Most enzyme have kcat values between 102 and 103 s-1 4. For simple reactions k2 = kcat , for mul ...
Naturally Occurring Ligand Isoforms Receptor Binding and Function
... hemopoietic growth factors, CSF-1 and steel factor (SLF) (1, 2), have demonstrated that the conformation of the extracellular domain is critical to maintaining function. Flt3 ligand, CSF-1, and SLF are all type 1 transmembrane proteins characterized by short cytoplasmic domains and four conserved cy ...
... hemopoietic growth factors, CSF-1 and steel factor (SLF) (1, 2), have demonstrated that the conformation of the extracellular domain is critical to maintaining function. Flt3 ligand, CSF-1, and SLF are all type 1 transmembrane proteins characterized by short cytoplasmic domains and four conserved cy ...
Cooperative binding
Molecular binding is an interaction between molecules that results in a stable physical association between those molecules. Cooperative binding occurs in binding systems that are constituted by more than one type (species) of molecule (say molecules A and B) and in which one of the partners is not mono-valent; i.e., it binds more than one molecule of the other molecular species. For example, one molecule of type A can bind 6 molecules of type B (in such cases, B is usually referred to as the ""ligand""). Binding in this type of system can be considered ""cooperative"" if the binding of B to one site on A is affected by the binding of B to other site(s) on A. In other words, the binding of B molecules to the different sites on A do not constitute mutually independent events. This can be due, for instance, to an affinity for the ligand that depends on the amount of ligand bound. Cooperativity can be positive or negative. Cooperative binding is observed in many biopolymers, including proteins and nucleic acids. Cooperative binding has been shown to be the mechanism underlying a large range of biochemical and physiological processes.