ACTIVE SITES OF HEMOPROTEINS
... (pKa 5.5) (Dasgupta et al., 1989). The hexa-coordinate ferric CCP is less reactive towards H2O2 than the penta-coordinate ferric CCP, due possibly to the rate-limiting dissociation of the sixth ligand (Dasgupta et al., 1989). The catalytic cycle of CCP involves two single one-electron oxidations of ...
... (pKa 5.5) (Dasgupta et al., 1989). The hexa-coordinate ferric CCP is less reactive towards H2O2 than the penta-coordinate ferric CCP, due possibly to the rate-limiting dissociation of the sixth ligand (Dasgupta et al., 1989). The catalytic cycle of CCP involves two single one-electron oxidations of ...
231. - Department of Chemistry
... N2, NO, CO2, N2O, and NO2. Reaction rate coefficients and product distributions were measured with the selected-ion flow tube technique operating at 294 ⫾ 3 K and a helium buffer-gas pressure of 0.35 ⫾ 0.01 Torr. The measurements provide intrinsic efficiencies for ligation and lead to intrinsic coor ...
... N2, NO, CO2, N2O, and NO2. Reaction rate coefficients and product distributions were measured with the selected-ion flow tube technique operating at 294 ⫾ 3 K and a helium buffer-gas pressure of 0.35 ⫾ 0.01 Torr. The measurements provide intrinsic efficiencies for ligation and lead to intrinsic coor ...
Immersive Projection for Biochemical Pattern Matching
... Structural Biology Unit, School of Biological Sciences ...
... Structural Biology Unit, School of Biological Sciences ...
Site-Directed Mutagenesis of Predicted Active Site Residues in
... Mental Health 5-T32-MH14275–21 (to B.K. Madras), and 1 F32 MH11895– 01 (to H.S.S.). ...
... Mental Health 5-T32-MH14275–21 (to B.K. Madras), and 1 F32 MH11895– 01 (to H.S.S.). ...
Allosteric Modulators of Steroid Hormone Receptors: Structural
... NR3C3); and the receptor for androgens (AR; NR3C4) (25). The receptors for vitamin D3 (VDR; NR1I1) and ecdysone (NR1H1) are found in subfamily I and act has heterodimers with retinoid X receptors (RXR) ␣, , or ␥ (NR2B1-3) or the insect homolog ultraspiracle, respectively. It is well established tha ...
... NR3C3); and the receptor for androgens (AR; NR3C4) (25). The receptors for vitamin D3 (VDR; NR1I1) and ecdysone (NR1H1) are found in subfamily I and act has heterodimers with retinoid X receptors (RXR) ␣, , or ␥ (NR2B1-3) or the insect homolog ultraspiracle, respectively. It is well established tha ...
INVESTIGATION INTO THE ALLOSTERIC REGULATION OF MITOTIC KINESIN EG5 Introduction Results
... Different allosteric inhibitors resulted in different inhibitory effects on KLP61F-L5 chimera. The two effectors may affect inhibition by operating through different allosteric communication networks. These different pathways for eliciting an inhibitory response are likely contingent upon the differ ...
... Different allosteric inhibitors resulted in different inhibitory effects on KLP61F-L5 chimera. The two effectors may affect inhibition by operating through different allosteric communication networks. These different pathways for eliciting an inhibitory response are likely contingent upon the differ ...
Ligand Conformation Enforces Trigonal
... A three-electrode system was used, consisting of a platinum working electrode, a platinum auxiliary electrode, and an Ag/AgCl reference electrode. The experiments were carried at room temperature under argon with tetrabutylammonium hexafluorophosphate as the electrolyte. All potentials are reported ...
... A three-electrode system was used, consisting of a platinum working electrode, a platinum auxiliary electrode, and an Ag/AgCl reference electrode. The experiments were carried at room temperature under argon with tetrabutylammonium hexafluorophosphate as the electrolyte. All potentials are reported ...
Pdf - Text of NPTEL IIT Video Lectures
... We are expressing here our conformation on a planar diagram. When you look at a three dimensional space, in a three dimensional space the one and three will not be in the same plane. The specificity can have a very large variety and one must also appreciate that the specificity of these enzymes in ...
... We are expressing here our conformation on a planar diagram. When you look at a three dimensional space, in a three dimensional space the one and three will not be in the same plane. The specificity can have a very large variety and one must also appreciate that the specificity of these enzymes in ...
Combining docking and molecular dynamic simulations in drug design
... or computational methods to use will depend on the characteristics of the system itself and the information available. In the present review, we cover the case where both the structure of the protein receptor and the binding site are known. While it is possible to develop drugs without such informat ...
... or computational methods to use will depend on the characteristics of the system itself and the information available. In the present review, we cover the case where both the structure of the protein receptor and the binding site are known. While it is possible to develop drugs without such informat ...
Alcohol Dehydrogenase
... • Ethanol is oxidized by ADH into acetaldehyde and NADH is formed • The highly toxic acetaldehyde is then further modified into foodstuffs for the cell • Throughout these processes, water molecules are lost and dehydration could set in after prolonged imbibing. ...
... • Ethanol is oxidized by ADH into acetaldehyde and NADH is formed • The highly toxic acetaldehyde is then further modified into foodstuffs for the cell • Throughout these processes, water molecules are lost and dehydration could set in after prolonged imbibing. ...
Understanding Hyaluronan-Protein Interactions
... activity, indicating that its hydroxyl group makes a crucial hydrogen bond to the sugar.6 Other amino acids of CD44, including three lysines, four arginines, two asparagines and a tyrosine (shown in orange on Figure 8), are also implicated in hyaluronan binding but have a less critical involvement. ...
... activity, indicating that its hydroxyl group makes a crucial hydrogen bond to the sugar.6 Other amino acids of CD44, including three lysines, four arginines, two asparagines and a tyrosine (shown in orange on Figure 8), are also implicated in hyaluronan binding but have a less critical involvement. ...
Localization of Leptin Binding Domain in the Leptin Receptor
... region of the cloned leptin receptor differs from that of many other cytokine receptors in that it contains two homologous segments representing potential ligand binding sites. After the analysis of various deletion and substitution mutants of the leptin receptor, we found that the first potential b ...
... region of the cloned leptin receptor differs from that of many other cytokine receptors in that it contains two homologous segments representing potential ligand binding sites. After the analysis of various deletion and substitution mutants of the leptin receptor, we found that the first potential b ...
The role of aromatic side chain residues in micelle binding by
... are the subject of continued interest and speculation. Small-angle neutron scattering studies [4] have shown that bile salt monomers are not dispersed over the entire surface of colipase in the complex, but in fact form micelle clusters distinct from and in juxtaposition to the protein molecule. Fro ...
... are the subject of continued interest and speculation. Small-angle neutron scattering studies [4] have shown that bile salt monomers are not dispersed over the entire surface of colipase in the complex, but in fact form micelle clusters distinct from and in juxtaposition to the protein molecule. Fro ...
The Biochemistry and Structural Biology of Plant
... endo), specificity for the sugar at the catalytic -1 subsite and more distal regions of the substrate ...
... endo), specificity for the sugar at the catalytic -1 subsite and more distal regions of the substrate ...
Document
... The first time we did an experiment with copper sulphate pentahydrate(CuSO4.5H2O) I was fascinated with it’s blue color. The colour of copper in metal form is brownish, and it’s colour in solution is blue. Although we know that reactants lose their properties when forming a product, I wondered why i ...
... The first time we did an experiment with copper sulphate pentahydrate(CuSO4.5H2O) I was fascinated with it’s blue color. The colour of copper in metal form is brownish, and it’s colour in solution is blue. Although we know that reactants lose their properties when forming a product, I wondered why i ...
Enzymes II – How Enzymes Work
... enzymes. Allosteric enzymes are enzymes with multiple binding sites. Often, these binding sites are found on different subunits of the enzyme. In other words, many allosteric enzymes have quaternary structure. Some of the binding sites on an allosteric enzyme are active, or catalytic, sites and bind ...
... enzymes. Allosteric enzymes are enzymes with multiple binding sites. Often, these binding sites are found on different subunits of the enzyme. In other words, many allosteric enzymes have quaternary structure. Some of the binding sites on an allosteric enzyme are active, or catalytic, sites and bind ...
Virtual Screening against Highly Charged Active Sites
... sequences; the 12 currently characterized functions, associated with nine experimentally solved structures, likely represent only a subset of the total functional diversity of the superfamily (5-7). Thus, for this large class of proteins, function must be assigned using information other than the kn ...
... sequences; the 12 currently characterized functions, associated with nine experimentally solved structures, likely represent only a subset of the total functional diversity of the superfamily (5-7). Thus, for this large class of proteins, function must be assigned using information other than the kn ...
Isoniazid Drug Resistance: Computational
... Isoniazid (INH), one of the effective anti-tuberculosis drugs, has been extensively used in TB control but several clinical strains of Mycobacterium tuberculosis (MTB) have shown resistance to INH. Besides, mutation in different MTB enzymes such as KatG, NAT, InhA, KasA, AhpC etc, over expression of ...
... Isoniazid (INH), one of the effective anti-tuberculosis drugs, has been extensively used in TB control but several clinical strains of Mycobacterium tuberculosis (MTB) have shown resistance to INH. Besides, mutation in different MTB enzymes such as KatG, NAT, InhA, KasA, AhpC etc, over expression of ...
F-actin Sequesters Elongation Factor from Interaction with
... et al., 1985, 1987). The interaction of Dictyostelium EF-Iot with F-actin is pH-dependent with a transition from tight to loose bundling between pH 6.7 and 7.6 (Edmonds et al., 1995). It has been proposed that pH may regulate the association of E F - l a with the cytoskeleton in such a way as to reg ...
... et al., 1985, 1987). The interaction of Dictyostelium EF-Iot with F-actin is pH-dependent with a transition from tight to loose bundling between pH 6.7 and 7.6 (Edmonds et al., 1995). It has been proposed that pH may regulate the association of E F - l a with the cytoskeleton in such a way as to reg ...
Coordination Chemistry of cis,trans-1,3,5
... unprecedented topology. Coordination to the open shell transition metals {NiCl2, Ni(NO3)2, Cu(NO3)2, CuBr2, CuCl2, CuF2, CuSO4} leads to discrete diligand complexes. Protonation of the copper(II) complexes predominantly forms monoligand species, which can aggregate into higher nuclearity clusters. P ...
... unprecedented topology. Coordination to the open shell transition metals {NiCl2, Ni(NO3)2, Cu(NO3)2, CuBr2, CuCl2, CuF2, CuSO4} leads to discrete diligand complexes. Protonation of the copper(II) complexes predominantly forms monoligand species, which can aggregate into higher nuclearity clusters. P ...
Recombinant Human Myoglobin
... Introduction: Myoglobin is a member of the globin superfamily and can be found in skeletal and cardiac muscles. It is a haemoprotein that contributes to intracellular oxygen storage and trans-cellular facilitated diffusion of oxygen. Myoglobin has a single-chain globular structure of 153 amino acids ...
... Introduction: Myoglobin is a member of the globin superfamily and can be found in skeletal and cardiac muscles. It is a haemoprotein that contributes to intracellular oxygen storage and trans-cellular facilitated diffusion of oxygen. Myoglobin has a single-chain globular structure of 153 amino acids ...
Microenvironment analysis and identification of magnesium binding
... could better pinpoint crystallographically observed Mg2+ binding sites. Because of their unique environment, sitebound Mg2+ ions were easier to positively predict than diffusely bound Mg2+ ions in complex RNA systems. Microenvironment analysis of Mg2+ binding To differentiate between site-bound and ...
... could better pinpoint crystallographically observed Mg2+ binding sites. Because of their unique environment, sitebound Mg2+ ions were easier to positively predict than diffusely bound Mg2+ ions in complex RNA systems. Microenvironment analysis of Mg2+ binding To differentiate between site-bound and ...
Acetylcholinesterase: A gorgeous enzyme It`s always said “Don`t
... Acetylcholinesterase: A gorgeous enzyme It’s always said “Don’t shoot the messenger”, but if the messenger keeps delivering the same message, he quickly becomes irritating! It is the job of the enzyme acetylcholinesterase (AChE) to ‘shoot the messenger’ at the neur ...
... Acetylcholinesterase: A gorgeous enzyme It’s always said “Don’t shoot the messenger”, but if the messenger keeps delivering the same message, he quickly becomes irritating! It is the job of the enzyme acetylcholinesterase (AChE) to ‘shoot the messenger’ at the neur ...
Cooperative binding
Molecular binding is an interaction between molecules that results in a stable physical association between those molecules. Cooperative binding occurs in binding systems that are constituted by more than one type (species) of molecule (say molecules A and B) and in which one of the partners is not mono-valent; i.e., it binds more than one molecule of the other molecular species. For example, one molecule of type A can bind 6 molecules of type B (in such cases, B is usually referred to as the ""ligand""). Binding in this type of system can be considered ""cooperative"" if the binding of B to one site on A is affected by the binding of B to other site(s) on A. In other words, the binding of B molecules to the different sites on A do not constitute mutually independent events. This can be due, for instance, to an affinity for the ligand that depends on the amount of ligand bound. Cooperativity can be positive or negative. Cooperative binding is observed in many biopolymers, including proteins and nucleic acids. Cooperative binding has been shown to be the mechanism underlying a large range of biochemical and physiological processes.