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C483 Study Guide for Exam 1
Summer 2017
Basic Information
 Exam 1: Tuesday, June 20th, 9:30-11:30AM in C033
 Arrive early for assigned seats
 Bring your student ID. Failure to do so will result in getting your exam back later.
 You may use a NON-PROGRAMMABLE calculator.
 All papers, books, phones, and electronic devices must be in a sealed bag under your seat.
Exam Content: The exam will cover chapters 1-6. All material covered in classnotes, book, and
homework could be on the exam. Details from case studies will not be included, but problems of
that sort are on the exam. Some major topics include:
Solubility, free energy/enthalpy/entropy, coupled reactions, intermolecular forces, solvation,
hydrophobic effect, amphipathic compounds, auto-ionization, pH, pKa, pH of strong acid
solutions, weak acids, Henderson-Hasselbalch, ionization state, titration curves, buffer capacity,
polyprotic acids, blood buffer, nucleobase, nucleoside, nucleotide, polynucleotide nomenclature,
base pairing, basis of double helix stability, denaturation and melting, RNA
structure/stability/function, transcription, translation, coding strand, restriction nucleases, amino
acid structures: stereochemistry and ionization state, essential amino acids, peptide bonds,
disulfide bonds, structural effects of amide resonance, -helix, Ramachandran plot, helical
wheel, -sheets, loops and turns, motifs, domains, thermodynamics of protein folding, size
exclusion chromatography, ion-exchange chromatography, affinity chromatography, SDSPAGE, isoelectric focusing, Edman Degradation, partial digestion, myoglobin/hemoglobin
structure-function, oxygen binding curve, hyperbolic vs sigmoidal curves, cooperativity, T vs R
conformation, 2,3-BPG, Bohr effect, examples of fibrous structural proteins, microfilaments and
microtubules, motor protein mechanisms, enzyme rate enhancement, recognizing 6 classes of
enzyme catalyzed reactions, chemical mechanisms of catalysis, covalent catalysis, pH effects on
enzymes, binding energy mechanisms, Lock and key vs induced fit, TS stabilization,
chymotrypsin, catalytic triad, substrate specificity
Mechanisms: Redox using NADH; triose phosphate isomerase, chymotrypsin
Exam Format: The exam is out of 100 points. The first 50 points will be fill in the blank,
true/false, and short answer problems. There will be four 10-point problems involving
calculations, structural drawings, or short essay. There will be a 10 point case study. A bonus
problem may or may not be included on the exam.