Regulation of Phenylalanine and Tyrosine
... buffers and protein solutions were supplemented with the protease inhibitor phenylmethanesulphonyl fluoride at a final concentration of 1 mM (Fahrney & Gold, 1963). Preparation of crude extracts. Frozen cells were ground with one and a half times their weight of aluminium oxide powder (Alcoa 305). T ...
... buffers and protein solutions were supplemented with the protease inhibitor phenylmethanesulphonyl fluoride at a final concentration of 1 mM (Fahrney & Gold, 1963). Preparation of crude extracts. Frozen cells were ground with one and a half times their weight of aluminium oxide powder (Alcoa 305). T ...
Quantitative Chemical Analysis
... My goals are to provide a sound physical understanding of the principles of analytical chemistry and to show how these principles are applied in chemistry and related disciplines— especially in life sciences and environmental science. I have attempted to present the subject in a rigorous, readable, ...
... My goals are to provide a sound physical understanding of the principles of analytical chemistry and to show how these principles are applied in chemistry and related disciplines— especially in life sciences and environmental science. I have attempted to present the subject in a rigorous, readable, ...
Purification and properties of NADP +-dependent
... required EDTA addition could not be combined with an anion exchange chromatography step. Finally, a number of different affinity matrices based on reactive dyes were tested. In crude extracts the A . niger enzyme did bind in the presence of 5mM-EDTA to Procion Red HE3Bagarose (Red A) from which it c ...
... required EDTA addition could not be combined with an anion exchange chromatography step. Finally, a number of different affinity matrices based on reactive dyes were tested. In crude extracts the A . niger enzyme did bind in the presence of 5mM-EDTA to Procion Red HE3Bagarose (Red A) from which it c ...
Isolation and Characterization of Protease Inhibitors from Animal
... macrodactylus is a 6.1-kDa Kunitz-type inhibitor with three S-S bonds but lacking Met and Trp residues. The inhibition constant values toward trypsin and -chymotrypsin were, respectively, 2.49 × 10-9 M and 2.17 × 10-8 M. The inhibitor displays a highly ordered tertiary structure and belongs to mixe ...
... macrodactylus is a 6.1-kDa Kunitz-type inhibitor with three S-S bonds but lacking Met and Trp residues. The inhibition constant values toward trypsin and -chymotrypsin were, respectively, 2.49 × 10-9 M and 2.17 × 10-8 M. The inhibitor displays a highly ordered tertiary structure and belongs to mixe ...
Affinity Precipitation of a Monoclonal Antibody ELP-Z Stimuli Responsive Biopolymer
... The ELP-Z based affinity precipitation process involves the binding of ELP-Z to the mAb followed by precipitation of the complex. The precipitates are then re-solubilized in the elution buffer where the mAb is eluted from the complex. This is followed by precipitation of the ELP-Z to recover the puri ...
... The ELP-Z based affinity precipitation process involves the binding of ELP-Z to the mAb followed by precipitation of the complex. The precipitates are then re-solubilized in the elution buffer where the mAb is eluted from the complex. This is followed by precipitation of the ELP-Z to recover the puri ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) ISSN: 2278-3008.
... pharmaceutical preparation has been created by the introduction of high performance liquid chromatography to the qualitative and quantitative analysis of peptides. (8) In Identification of multiple peptide and proteins (i.e. huge molecules) we must used HPLC with two pumps (binary pump) in order to ...
... pharmaceutical preparation has been created by the introduction of high performance liquid chromatography to the qualitative and quantitative analysis of peptides. (8) In Identification of multiple peptide and proteins (i.e. huge molecules) we must used HPLC with two pumps (binary pump) in order to ...
Purification and Physico-Chemical Analysis of Fractions from the
... completely the major components of fractions S I . 1 and S I . 2 (Fig. 2) by gel filtration alone, chromatographic separation on columns of DEAE-cellulose and DEAESephadex was investigated. Of these two anion-exchangers, DEAE-cellulose proved to be the more suitable in practice. The first purificati ...
... completely the major components of fractions S I . 1 and S I . 2 (Fig. 2) by gel filtration alone, chromatographic separation on columns of DEAE-cellulose and DEAESephadex was investigated. Of these two anion-exchangers, DEAE-cellulose proved to be the more suitable in practice. The first purificati ...
bile acids - The Vespiary
... that solubilize cholesterol in the bile. The primary bile acids, cholic acid and chenodeoxycholic acid, are effectively reabsorbed from ileum during their enterohepatic circulation, but approximately 5% that escape reabsorption seep into the colon, and are subjected to modiRcation to secondary bile ...
... that solubilize cholesterol in the bile. The primary bile acids, cholic acid and chenodeoxycholic acid, are effectively reabsorbed from ileum during their enterohepatic circulation, but approximately 5% that escape reabsorption seep into the colon, and are subjected to modiRcation to secondary bile ...
Purification of Arrestin from Bovine Retinas
... The binding of arrestin (also referred to as the 48K protein or S-antigen) to freshly bleached phosphorylated rhodopsin occurs rapidly (<200 msec) and quenches the activation of G protein (transducin) (1-4). We have shown that arrestin also acts in the phototransduction process by blocking rapid dep ...
... The binding of arrestin (also referred to as the 48K protein or S-antigen) to freshly bleached phosphorylated rhodopsin occurs rapidly (<200 msec) and quenches the activation of G protein (transducin) (1-4). We have shown that arrestin also acts in the phototransduction process by blocking rapid dep ...
Concept based notes Chemistry Lab Manual
... through a stationary phase under the influence of a mobile phase. Q. 90. Name some chromatography techniques? Ans. Paper chromatography, column chromatography, thin layer chromatography, Gas chromatography and high pressure liquid chromatography. Q. 91. What is paper chromatography? Ans. It is an im ...
... through a stationary phase under the influence of a mobile phase. Q. 90. Name some chromatography techniques? Ans. Paper chromatography, column chromatography, thin layer chromatography, Gas chromatography and high pressure liquid chromatography. Q. 91. What is paper chromatography? Ans. It is an im ...
circular paper chromatography 95 - Journal of the Indian Institute of
... this method was developed and briefly described before by Giri and Rao (1952). Further details of this technique are described below. A small circle of about 4 cm. diameter wa, drawn with a pencil from the centre of a circuIrrr filter-paper. The soh!tion (about 1-·8{"1.) containing mixture of amino ...
... this method was developed and briefly described before by Giri and Rao (1952). Further details of this technique are described below. A small circle of about 4 cm. diameter wa, drawn with a pencil from the centre of a circuIrrr filter-paper. The soh!tion (about 1-·8{"1.) containing mixture of amino ...
Supp Mat - Columbia University
... II release factors, or ribosome recycling factor was present in the translation reactions; thus, translation reactions are expected to be single turnover under our experimental conditions. After quenching, f-[3H]Met amino acid was separated from f-[3H]Met-X dipeptide by analytical high performance l ...
... II release factors, or ribosome recycling factor was present in the translation reactions; thus, translation reactions are expected to be single turnover under our experimental conditions. After quenching, f-[3H]Met amino acid was separated from f-[3H]Met-X dipeptide by analytical high performance l ...
Isolation of Rat Transferrin Using CNBr-Activated
... Isoelectric focusing was performed in the 110 ml Ampholine Column (L.K.B., 8101). The medium was composed of a 1% ampholyte solution (Ampholine Carrier Ampholytes, L.K.B.) in the pH range of about 5.6-6.1 to which 0.1% ampholytes (L.k.B.) in the pH range of 3-10 was added. The solution was introduce ...
... Isoelectric focusing was performed in the 110 ml Ampholine Column (L.K.B., 8101). The medium was composed of a 1% ampholyte solution (Ampholine Carrier Ampholytes, L.K.B.) in the pH range of about 5.6-6.1 to which 0.1% ampholytes (L.k.B.) in the pH range of 3-10 was added. The solution was introduce ...
Comparison of cell-wall teichoic acid with high-molecular
... The material eluted from Concanavalin-ASepharose 4-B with 0.1 M methyl a-D-glucoside in buffer was hydrolysed in 6 M HC1 for 18 h in a sealed tube, and after removing acid was analysed by paper and thin-layer chromatography in two dimensions. A single ninhydrin-positive spot with an R, value corresp ...
... The material eluted from Concanavalin-ASepharose 4-B with 0.1 M methyl a-D-glucoside in buffer was hydrolysed in 6 M HC1 for 18 h in a sealed tube, and after removing acid was analysed by paper and thin-layer chromatography in two dimensions. A single ninhydrin-positive spot with an R, value corresp ...
Isolation of All Soluble Tryptic Peptides from the α Polypeptide
... Column Chromatography of the Soluble Tryptic Peptides on Dowex 1 x 2 500 ml of dry resin (Dowex 1 x 2, 200-400 mesh, Cl-form, Dow Chemical Company) was suspended in three volumes of deionized water. The suspension, after stirred, was allowed to stand for an hour and then the fine particles discarded ...
... Column Chromatography of the Soluble Tryptic Peptides on Dowex 1 x 2 500 ml of dry resin (Dowex 1 x 2, 200-400 mesh, Cl-form, Dow Chemical Company) was suspended in three volumes of deionized water. The suspension, after stirred, was allowed to stand for an hour and then the fine particles discarded ...
Drugs of Abuse in Urine by GC/MS Following SAMHSA (NIDA
... The GC/MS analysis of 6-mono-acetyl morphine following the procedure presented below has demonstrated method limits of quantitation at 10 ng/mL and limits of detection in urine at 1 ng/mL. The analysis of MAM requires the use of silanized glassware to prevent loss of analytes. Furthermore, the analy ...
... The GC/MS analysis of 6-mono-acetyl morphine following the procedure presented below has demonstrated method limits of quantitation at 10 ng/mL and limits of detection in urine at 1 ng/mL. The analysis of MAM requires the use of silanized glassware to prevent loss of analytes. Furthermore, the analy ...
recombinant protein characterization
... Appropriate analytical techniques are essential to effective process monitoring during the development and manufacturing of a protein pharmaceutical. The demand for rapid and effective monitoring techniques is being driven by the need to better understand the biological production processes, as well ...
... Appropriate analytical techniques are essential to effective process monitoring during the development and manufacturing of a protein pharmaceutical. The demand for rapid and effective monitoring techniques is being driven by the need to better understand the biological production processes, as well ...
analytical chemistry - Львівський національний медичний
... 4) analysis in drops on filter paper – reaction between analysed substance and analytical reagent run on filter paper with some drops (1-2) of solutions – arise a coloured spots. Requirements (demands) to analytical reactions: 1) reaction must run quickly, in practice – immediately; 2) reaction mus ...
... 4) analysis in drops on filter paper – reaction between analysed substance and analytical reagent run on filter paper with some drops (1-2) of solutions – arise a coloured spots. Requirements (demands) to analytical reactions: 1) reaction must run quickly, in practice – immediately; 2) reaction mus ...
HIS-Select Nickel Affinity Gel (P6611) - Technical - Sigma
... The HIS-Select Nickel Affinity Gel is stored in 30% ethanol. The ethanol must be removed just prior to use. Thoroughly resuspend the affinity gel with gentle inversion and remove an appropriate aliquot for use. Take only the amount of affinity gel that is necessary for the purification to be done. T ...
... The HIS-Select Nickel Affinity Gel is stored in 30% ethanol. The ethanol must be removed just prior to use. Thoroughly resuspend the affinity gel with gentle inversion and remove an appropriate aliquot for use. Take only the amount of affinity gel that is necessary for the purification to be done. T ...
Effect of surface hydrophobicity distribution on retention
... to use this technique because it is the only hold-up volume measurement with a physical meaning, as it represents the volume within the column accessible to the two solvents and an analyte molecule of a size comparable to that of the solvent molecule [18]. 3.4. Experimental conditions Isocratic HIC ...
... to use this technique because it is the only hold-up volume measurement with a physical meaning, as it represents the volume within the column accessible to the two solvents and an analyte molecule of a size comparable to that of the solvent molecule [18]. 3.4. Experimental conditions Isocratic HIC ...
Characterization of Lamprey Fibrinopeptides
... with hot water and filtered with a stemless funnel just before use. Tubes were sealed immediately. The hydrolysate (usually 50 ,ul.) was applied directly to the paperelectrophoresis strips for analysis by combination paper electrophoresis-paper chromatography. The applications were made to the dry p ...
... with hot water and filtered with a stemless funnel just before use. Tubes were sealed immediately. The hydrolysate (usually 50 ,ul.) was applied directly to the paperelectrophoresis strips for analysis by combination paper electrophoresis-paper chromatography. The applications were made to the dry p ...
Purification and Partial Characterization of a Latent Serine Protease
... could not be calculated until the last step of purification, because the crude extract contained many other proteases which are active against casein and because the final Superose-12 column step eventually separates LSP from the unidentified, low molecular weight proteolytic activity. In a number o ...
... could not be calculated until the last step of purification, because the crude extract contained many other proteases which are active against casein and because the final Superose-12 column step eventually separates LSP from the unidentified, low molecular weight proteolytic activity. In a number o ...
Partial Purification and Characterization of Three Flavonol
... aqueous layer of the assay mixture (20). An aliquot of the organic phase was counted for radioactivity in a toluene-based scintillation fluid. The remaining fraction was concentrated under N2 and used for identification of the reaction products by cochromatography with reference compounds (21). TLC ...
... aqueous layer of the assay mixture (20). An aliquot of the organic phase was counted for radioactivity in a toluene-based scintillation fluid. The remaining fraction was concentrated under N2 and used for identification of the reaction products by cochromatography with reference compounds (21). TLC ...
chromatographic study of photolysis of aqueous cyanocobalamin
... of their spots increased with an increase in pH indicating that their rate of formation is pH dependent. Riboflavin (solvent systems S5 - S6) showed extensive degradation with an increase in pH. The photoproducts detected in these reactions included formylmethylflavin and lumichrome (major) at pH 2- ...
... of their spots increased with an increase in pH indicating that their rate of formation is pH dependent. Riboflavin (solvent systems S5 - S6) showed extensive degradation with an increase in pH. The photoproducts detected in these reactions included formylmethylflavin and lumichrome (major) at pH 2- ...
Phytochemistry 24:
... of S-ADP-Agarose in purification of pea seed GS [25]; possibly, the use of Mg2+ rather than Mn2+ ions in the buffer may explain their result. Dowton and Kennedy (unpublished) have successfully employed 2’,5’-ADPSepharose chromatography to purify an insect GS and this affinity procedure may prove of ...
... of S-ADP-Agarose in purification of pea seed GS [25]; possibly, the use of Mg2+ rather than Mn2+ ions in the buffer may explain their result. Dowton and Kennedy (unpublished) have successfully employed 2’,5’-ADPSepharose chromatography to purify an insect GS and this affinity procedure may prove of ...
Chromatography
Chromatography (/ˌkroʊməˈtɒɡrəfi/; from Greek χρῶμα chroma which means ""color"" and γράφειν graphein ""to write"") is the collective term for a set of laboratory techniques for the separation of mixtures.The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate. The separation is based on differential partitioning between the mobile and stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus changing the separation.Chromatography may be preparative or analytical. The purpose of preparative chromatography is to separate the components of a mixture for more advanced use (and is thus a form of purification). Analytical chromatography is done normally with smaller amounts of material and is for measuring the relative proportions of analytes in a mixture. The two are not mutually exclusive.