Purification and some properties of UDP
... of about 71 kDa and 32 kDa, respectively, whereas the xylosyltransferases from rat chondrosarcoma and from embryonic chick cartilage seem to be tetrameric structures composed of two pairs of nonidentical subunits of 23 kDa and 27 kDa, respectively. Beside different origin of the enzymes, the prepara ...
... of about 71 kDa and 32 kDa, respectively, whereas the xylosyltransferases from rat chondrosarcoma and from embryonic chick cartilage seem to be tetrameric structures composed of two pairs of nonidentical subunits of 23 kDa and 27 kDa, respectively. Beside different origin of the enzymes, the prepara ...
Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents
... Usually, increasing the concentration of organic modifier in the eluent in the reversed phase chromatography leads to reduction of the retention times of the solutes because of an increasing solvent strength. The influence of methanol as modifier was investigated with the aliphatic amino acids Val, ...
... Usually, increasing the concentration of organic modifier in the eluent in the reversed phase chromatography leads to reduction of the retention times of the solutes because of an increasing solvent strength. The influence of methanol as modifier was investigated with the aliphatic amino acids Val, ...
HiTrap Chelating HP 1 ml and 5 ml
... Metal chelate affinity chromatography separates proteins and peptides on the basis of their affinity for metal ions that have been immobilized by chelation. Certain amino acids (e.g. histidine and cysteine) form complexes with the chelated metals around neutral pH (pH 6–8). It is primarily the histi ...
... Metal chelate affinity chromatography separates proteins and peptides on the basis of their affinity for metal ions that have been immobilized by chelation. Certain amino acids (e.g. histidine and cysteine) form complexes with the chelated metals around neutral pH (pH 6–8). It is primarily the histi ...
HiTrap Heparin HP,1 ml and 5 ml
... pH stability, long term refers to the pH interval where the medium is stable over a long period of time without adverse effects on its subsequent chromatographic performance. pH stability, short term refers to the pH interval for regeneration, cleaning-in-place and sanitization procedure ...
... pH stability, long term refers to the pH interval where the medium is stable over a long period of time without adverse effects on its subsequent chromatographic performance. pH stability, short term refers to the pH interval for regeneration, cleaning-in-place and sanitization procedure ...
Protein Purification and Analysis
... Important steps in chromatography 1. Pack column - Column is packed with material (resin) that can absorb molecules based on some property (charge, size, binding affinity, etc.) 2. Wash column - Molecules washed through the column with buffer 3. Collect fractions - Fractions are taken, at some point ...
... Important steps in chromatography 1. Pack column - Column is packed with material (resin) that can absorb molecules based on some property (charge, size, binding affinity, etc.) 2. Wash column - Molecules washed through the column with buffer 3. Collect fractions - Fractions are taken, at some point ...
No Slide Title
... Important steps in chromatography 1. Pack column - Column is packed with material (resin) that can absorb molecules based on some property (charge, size, binding affinity, etc.) 2. Wash column - Molecules washed through the column with buffer 3. Collect fractions - Fractions are taken, at some point ...
... Important steps in chromatography 1. Pack column - Column is packed with material (resin) that can absorb molecules based on some property (charge, size, binding affinity, etc.) 2. Wash column - Molecules washed through the column with buffer 3. Collect fractions - Fractions are taken, at some point ...
Valine Mydrogenase from Streptmzyces fiadipe
... NAD+analoguesand NADPH were purchased from Sigma. Phenyl-Sephardse CL-4B and the Mono Q HR 5/5 prepacked fast protein liquid chromatography (FPLC) column were from Pharmacia. All other chemicals were of the highest purity available. Micrwrganism and growth conditions. The micro-organism used was Str ...
... NAD+analoguesand NADPH were purchased from Sigma. Phenyl-Sephardse CL-4B and the Mono Q HR 5/5 prepacked fast protein liquid chromatography (FPLC) column were from Pharmacia. All other chemicals were of the highest purity available. Micrwrganism and growth conditions. The micro-organism used was Str ...
Characterization of Multi-constituent Substances for REACH
... challenge. The successful analysis of multi-constituent substances requires a combination of in-depth knowledge of the chemical processes used to prepare them and a well-chosen suite of tests. ECHA’s Guidance for Identification and Naming of Substances under REACH defines two possible registration r ...
... challenge. The successful analysis of multi-constituent substances requires a combination of in-depth knowledge of the chemical processes used to prepare them and a well-chosen suite of tests. ECHA’s Guidance for Identification and Naming of Substances under REACH defines two possible registration r ...
F.Y. B.Sc. - Vocational Biotechnology
... Various methods staining and other basic microbiological techniques Microbiological analysis of various water samples. ...
... Various methods staining and other basic microbiological techniques Microbiological analysis of various water samples. ...
Allied Biochemistry II - E
... 1. Chromatography is used to separate A. solution B. mixtures C. molecules D. atoms 2. Chromatography with solid stationary phase is called A. circle chromatography B. Square chromatography C. solid chromatography D. adsorption chromatography 3. Pattern on paper in chromatography is called A. chromi ...
... 1. Chromatography is used to separate A. solution B. mixtures C. molecules D. atoms 2. Chromatography with solid stationary phase is called A. circle chromatography B. Square chromatography C. solid chromatography D. adsorption chromatography 3. Pattern on paper in chromatography is called A. chromi ...
Thin-Layer Chromatography of Amino Acids
... chromatography, gas chromatography, liquid chromatography, and thinlayer chromatography. Thin Layer Chromatography (TLC) is used to separate solids from a liquid. The most common use is to separate amino acids from a liquid and each other. A spot of the sample is placed on a sheet of glass treated w ...
... chromatography, gas chromatography, liquid chromatography, and thinlayer chromatography. Thin Layer Chromatography (TLC) is used to separate solids from a liquid. The most common use is to separate amino acids from a liquid and each other. A spot of the sample is placed on a sheet of glass treated w ...
TECHNICAL NOTES Aurich, H .
... the amides at pH 2.0 by stewning for 30 min. After hydrolysis, anrmnio and glutamic + clspartic acid ore increased. Concentmticms of glutcmic cmd aspartic acid measured ore the sums of free acids ar.d their omides( individual amaunh can be calculated from differences analyzed before and after hydrol ...
... the amides at pH 2.0 by stewning for 30 min. After hydrolysis, anrmnio and glutamic + clspartic acid ore increased. Concentmticms of glutcmic cmd aspartic acid measured ore the sums of free acids ar.d their omides( individual amaunh can be calculated from differences analyzed before and after hydrol ...
Rapid purification of heart muscle enzymes using dye affinity
... Previous workers have demonstrated differences between the use of reactive dye ligands immobilised to solid mahices and in aqueous twophase systems [4]. It has been proposed that the proteidmahix interactions may also be in,olved during column chromatography [ 5 ] , while aqueous two-phase systems p ...
... Previous workers have demonstrated differences between the use of reactive dye ligands immobilised to solid mahices and in aqueous twophase systems [4]. It has been proposed that the proteidmahix interactions may also be in,olved during column chromatography [ 5 ] , while aqueous two-phase systems p ...
1.0 amino acids as units of protein structure
... chosen protein so that they can study its specific properties in the absence of other proteins. Because the biological function of a protein depends on its native structure, techniques employed in protein purification should not denature the protein. The starting material for protein purification is ...
... chosen protein so that they can study its specific properties in the absence of other proteins. Because the biological function of a protein depends on its native structure, techniques employed in protein purification should not denature the protein. The starting material for protein purification is ...
COMPARATIVE ANIMAL PHYSIOLOGY (Level 2, 3 CU) a. Brief
... COMPARATIVE ANIMAL PHYSIOLOGY (Level 2, 3 CU) a. Brief Course Description This course covers organismic and population physiology. Phylogenic approach to the study of systems integrating invertebrate and vertebrate body functions in relation to environmental conditions; homeostasis; coordination of ...
... COMPARATIVE ANIMAL PHYSIOLOGY (Level 2, 3 CU) a. Brief Course Description This course covers organismic and population physiology. Phylogenic approach to the study of systems integrating invertebrate and vertebrate body functions in relation to environmental conditions; homeostasis; coordination of ...
Determination of Fatty Acids and Carbohydrate Monomers in Micro
... scarcely separate the three hexoses. Furthermore, peaks of tuberculostearic acid and 2-eicosanol could not be separated. The capillary column in the present study, which used the same stationary phase (viz. OV-101) as the two previous studies, enabled all these compounds to be separated. This statio ...
... scarcely separate the three hexoses. Furthermore, peaks of tuberculostearic acid and 2-eicosanol could not be separated. The capillary column in the present study, which used the same stationary phase (viz. OV-101) as the two previous studies, enabled all these compounds to be separated. This statio ...
Determination of Fatty Acids and Carbohydrate Monomers in Micro
... scarcely separate the three hexoses. Furthermore, peaks of tuberculostearic acid and 2-eicosanol could not be separated. The capillary column in the present study, which used the same stationary phase (viz. OV-101) as the two previous studies, enabled all these compounds to be separated. This statio ...
... scarcely separate the three hexoses. Furthermore, peaks of tuberculostearic acid and 2-eicosanol could not be separated. The capillary column in the present study, which used the same stationary phase (viz. OV-101) as the two previous studies, enabled all these compounds to be separated. This statio ...
to view or the PHOTOSYNTHESIS Presentation
... Procedure Mark how far up the paper the alcohol traveled with a pencil Determine how many bands of pigment you have, what color they are, and measure how far each band traveled from the origin line ...
... Procedure Mark how far up the paper the alcohol traveled with a pencil Determine how many bands of pigment you have, what color they are, and measure how far each band traveled from the origin line ...
03_Physical-chemical properties of proteins
... acids on the basis of differences in absorption, ionic charges, size and solubility of molecules Electrophoresis – effects separation in an electric field on the basis of differences in charges carried by amino acids and proteins under specific condition Ultracentrifugation – effects separation on t ...
... acids on the basis of differences in absorption, ionic charges, size and solubility of molecules Electrophoresis – effects separation in an electric field on the basis of differences in charges carried by amino acids and proteins under specific condition Ultracentrifugation – effects separation on t ...
Dr. Atiya Abbasi Lecture 04_ IEC_ 16 Jan.ppt
... of combining resolution with speed during the same separation. A knowledge of the chromatographic behavior of the sample obtained from previous separations using simpler gradients is essential. ...
... of combining resolution with speed during the same separation. A knowledge of the chromatographic behavior of the sample obtained from previous separations using simpler gradients is essential. ...
Document
... Chromatography and electrophoresis of proteins For a protein to be assayed by X-ray crystallography or protein sequencing, a pure sample must be produced. After preparation of a cell extract, an appropriate separation method must be employed. Two such methods are: Chromatography Electrophoresis ...
... Chromatography and electrophoresis of proteins For a protein to be assayed by X-ray crystallography or protein sequencing, a pure sample must be produced. After preparation of a cell extract, an appropriate separation method must be employed. Two such methods are: Chromatography Electrophoresis ...
IEX and RP Method Development for the Separation of
... Sample is injected in a mobile phase buffer with a low salt concentration – this binds proteins to the column. Proteins are typically eluted at constant pH with increasing salt gradients (mobile-phase ionic strength) to displace the proteins from the stationary phase. ...
... Sample is injected in a mobile phase buffer with a low salt concentration – this binds proteins to the column. Proteins are typically eluted at constant pH with increasing salt gradients (mobile-phase ionic strength) to displace the proteins from the stationary phase. ...
powerpoint
... • Because the N-terminus of a peptide chain is distict from the C-terminus, a small peptide composed of different aminoacids may have a several constitutional isomers (e.g. Asp-Phe or Phe-Asp). • The methyl ester of the first dipeptide (structure on the right) is the artificial sweetner aspartame, w ...
... • Because the N-terminus of a peptide chain is distict from the C-terminus, a small peptide composed of different aminoacids may have a several constitutional isomers (e.g. Asp-Phe or Phe-Asp). • The methyl ester of the first dipeptide (structure on the right) is the artificial sweetner aspartame, w ...
Proteomics pathway Most common properties of proteins
... alternative approaches of enrichment of rare polypeptides may be used: Subcellular fractionation and protein prefractionation. separate membrane proteins, nucleole, cytoplasmic, cytosolics, etc. Specific reagents to increase solubility of hydrophobic proteins. Specific reagents to break disulf ...
... alternative approaches of enrichment of rare polypeptides may be used: Subcellular fractionation and protein prefractionation. separate membrane proteins, nucleole, cytoplasmic, cytosolics, etc. Specific reagents to increase solubility of hydrophobic proteins. Specific reagents to break disulf ...
Chromatography
Chromatography (/ˌkroʊməˈtɒɡrəfi/; from Greek χρῶμα chroma which means ""color"" and γράφειν graphein ""to write"") is the collective term for a set of laboratory techniques for the separation of mixtures.The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate. The separation is based on differential partitioning between the mobile and stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus changing the separation.Chromatography may be preparative or analytical. The purpose of preparative chromatography is to separate the components of a mixture for more advanced use (and is thus a form of purification). Analytical chromatography is done normally with smaller amounts of material and is for measuring the relative proportions of analytes in a mixture. The two are not mutually exclusive.