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ORIGINAL RESEARCH ARTICLE
International Journal of Current Medical And Applied Sciences.
[IJCMAAS]
Volume: 12, Issue: 1, September -2016, pp: 14-19.
DNA Extraction by Perchlorate Method.
A. K. Harith1, K. Bhatia2 & D. Agarwal3
Classified Specialist (Pathology and Biochemistry) , Command Hospital, Pune [MS], India.
Professor, 3Gd Specialist, Department of Biochemistry, Armed Forces Medical College, Pune [MS], India.
1
2Assistant
--------------------------------------------------------------------------------------------------------------------------------------Abstract:
Introduction: The quality and quantity of DNA extracted from cell suspension is a critical aspect while performing
molecular biology tests. Most of the laboratories are using kit based DNA extraction methods, which is expensive.
We compared the kit based DNA extraction with a conventional technique of DNA extraction based on the
Perchlorate technique.
Material and Method: DNA was extracted on 60 samples by the kit based techniques and the Perchlorate Method
(PC method) and compared for both quality and quantity of DNA extracted. DNA was also extracted from 15
samples with a total leukocyte count of less than 3000/cmm and compared, as it was felt that the yield of DNA from
the kit method in such samples would be low.
Results: The yield of DNA by the kit method was 53 ± 6.2 ng/µL while that of the PC method was 431± 67 ng/µL. On
an average the PC method had a better yield of DNA as compared to the kit method. In samples with low TLC count,
the yield of DNA by the conventional method was significantly better than that of the kit method (119± 44 ng/µL vs
23 ± 7.2 ng/µL).
Conclusion: The PC method provides high yield of good quality DNA at a more economical rate as compared to the
kit based methods.
--------------------------------------------------------------------------------------------------------------------------------------------------------------------Address for correspondence:
Dr. A. K. Harith,
Access this Article Online
Classified Specialist,
Department of Biochemistry,
Website:
Command Hospital,
Pune, [MS] , India.
www.ijcmaas.com
Email ID: [email protected]
[email protected]
How to cite this article:
A. K. Harith, K. Bhatia & D. Agarwal: DNA Extraction by Perchlorate
Method.. : International Journal of current Medical and Applied
sciences; 2016, 12(1), 14-19 .
.
.
Subject:
Medical Sciences
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