Download Hands-on Exercise: Locating Protein Information

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Hands-on Exercise: Locating Protein Information
January 13, 2010
Literature Informatics:
Which proteins are related to Alzheimer’s disease?
Where/who are the leading centers and scientists for Alzheimer’s research?
Protein-centric Information:
Identify the human protein containing this short peptide sequence:
What is its function?
What is its calculated PI and molecular wt?
Which region of this protein is most hydrophobic?
Locate five experimentally verified Ser/Thr/Tyr phosphorylation sites in this protein.
Find the homologous mouse and fruit fly orthologs of this human protein and report the
% protein shared identity.
How many protein domains are reported to be present in this human protein?
Find the location of its largest domain.
View the crystal structure of Chronophin (PDB entry: 2P69).
A variant of this protein with mutations in its amino acid sequence has been isolated
(see link
Can you predict any effect the mutations may have on its function?
Hint: Identify the amino acid residues which are in close contact (3.5 A) with
Bioinformatics Resources:
Locate database(s) on G protein coupled receptors
Find a suitable protocol for the expression of GST fusion proteins