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Transcript
Hands-on Exercise: Locating Protein Information
January 13, 2010
Literature Informatics:

Which proteins are related to Alzheimer’s disease?

Where/who are the leading centers and scientists for Alzheimer’s research?
Protein-centric Information:

Identify the human protein containing this short peptide sequence:
GPDGMPVIYHGHTLTTKIKFSDVLHTIKE
o
What is its function?
o
What is its calculated PI and molecular wt?
o
Which region of this protein is most hydrophobic?
o
Locate five experimentally verified Ser/Thr/Tyr phosphorylation sites in this protein.
o
Find the homologous mouse and fruit fly orthologs of this human protein and report the
% protein shared identity.
o
How many protein domains are reported to be present in this human protein?
o
Find the location of its largest domain.
Structure:

View the crystal structure of Chronophin (PDB entry: 2P69).
o
A variant of this protein with mutations in its amino acid sequence has been isolated
(see link http://www.hsls.pitt.edu/guides/genetics/tutorials).
o
Can you predict any effect the mutations may have on its function?
o
Hint: Identify the amino acid residues which are in close contact (3.5 A) with
PYRIDOXAL-5'-PHOSPHATE (PLP).
Bioinformatics Resources:

Locate database(s) on G protein coupled receptors

Find a suitable protocol for the expression of GST fusion proteins