Download TABLE 8–6 Some Reagents Commonly Used to Cleave Peptide

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Transcript
TABLE 8–6 Some Reagents Commonly Used to Cleave Peptide Bonds
in Proteins
AMINO ACID 1
Enzyme
Trypsin
Lys or Arg
Chymotrypsin
Phe, Trp, or Tyr
V8 protease
Glu
Chemical
Cyanogen bromide
Met
2-Nitro-5-thiocyanobenzoate
any
AMINO ACID 2
any
any
any
any
Cys
The specificity for the amino acids on either side of the cleaved bond is indicated; amino
acid 2 is linked to the C-terminus of amino acid 1.