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CHEM 109C – Zhang
CLAS, F10
Coenzymes II: Pyridoxal Phosphate (PLP)
1. (Ch 24, #14) What amino acids are formed from the following a-ketoacids in
PLP-catalyzed transaminations?
a. Pyruvate
b. Oxaloacetate
2. (Ch 24, #16) Which of the following compounds is more easily decarboxylated?
O
O
-
O
O
+
+
N
N
a.
H
-
b.
H
3. (Ch 24, #17) Explain why the ability of PLP to catalyze an amino acid
transformation is greatly reduced if a PLP-requiring enzymatic reaction is carried
out at a pH at which the pyridine nitrogen is not protonated.
4. (Ch 24, #18) Explain why the ability of PLP to catalyze an amino acid
transformation is greatly reduced if the OH substitiuent of the pyridoxal
phosphate is replaced by a methyoxy group.
5. Which are the 5 types of reactions that we have learned that PLP catalyzes?
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