Download practice making a protein from dna

PRACTICE MAKING A PROTEIN FROM DNA
Here is the double stranded DNA. It will be making a very short protein.
5'
3'
... A C A T G G A T T A C A C C T G G A A G T A G C A T ... 3'
... T G T A C C T A A T G T G G A C C G T C A T C G T A ... 5'
sense strand
antisense strand
transcription of antisense strand
mRNA:
(then some processing happens)
(RNA goes from nucleus to ribosome)
Protein:
translation of mRNA to protein
(i)
(ii)
(iii)
read along the sense strand of DNA until you find ATG. This is the start of all genes.
Circle the "A" in ATG so you know where to start.
RNA is copied from the antisense strand. So write the mRNA letters that are opposite to
the antisense strand. (e.g where you see a T write an A, A  U, C  G, G  C)
(iv)
(v)
(vi)
The starting codon is now AUG in the mRNA
On your RNA strand, make a vertical line every 3 bases (letters).
Look up each 3 letter codon on the table of amino acids and write down the three letter
abbreviation for each amino acid. Do this next to the word "Protein"
(Amino acids can be written as words or abbreviations like this: Arginine or Arg or R)
It should look like MET - ARG - ... - ... - GLN STOP
(but it will have other, different amino acids.).
If you’ve done it correctly, there will be 6 amino acids, then a STOP codon.
(vii)
If you want to do another one imagine the the DNA is flipped around, so read right to
lift from the 5' end of the anti-sense strand and you’ll come up with another protein.
It doesn’t have a stop code in this sequence.
RNA Codon table
2nd base
1st
base
U
C
A
G
U
UUU
UUC
UUA
UUG
CUU
CUC
CUA
CUG
AUU
AUC
AUA
AUG*
GUU
GUC
GUA
GUG
(Phe / F) Phenylalanine
(Leu / L) Leucine
(Ile / I) Isoleucine
(Met / M) Methionine
(Val / V) Valine
UCU
UCC
UCA
UCG
CCU
CCC
CCA
CCG
ACU
ACC
ACA
ACG
GCU
GCC
GCA
GCG
3rd
base
C
A
G
UAU
UGU
U
(Tyr / Y) Tyrosine
(Cys / C) Cysteine
UAC
UGC
C
(Ser / S) Serine
UAA Stop
UGA Stop
A
UAG Stop
UGG (Trp / W) Tryptophan
G
CAU
CGU
U
(His / H) Histidine
CAC
CGC
C
(Pro / P) Proline
(Arg / R) Arginine
CAA
CGA
A
(Gln / Q) Glutamine
CAG
CGG
G
AAU
AGU
U
(Asn / N) Asparagine
(Ser / S) Serine
AAC
AGC
C
(Thr / T) Threonine
AAA
AGA
A
(Lys / K) Lysine
(Arg / R) Arginine
AAG
AGG
G
GAU
GGU
U
(Asp / D) Aspartic acid
GAC
GGC
C
(Ala / A) Alanine
(Gly / G) Glycine
GAA
GGA
A
(Glu/E) Glutamic acid
GAG
GGG
G
The codon AUG both codes for methionine and serves as an initiation site (or start codon): the first AUG in an mRNA's coding region is where
translation into protein begins. (from https://en.wikipedia.org/wiki/Genetic_code#RNA_codon_table)
Here is human hemoglobin subunit beta (using the one-letter abbrev. for amino acids):
MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPK
VKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFG
KEFTPPVQAAYQKVVAGVANALAHKYH