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Cell Transport Movement of substances in and out of cells Substances move in and out of cells by: 1. Passive Transport 2. Active Transport Passive Transport No energy (ATP) is required to move gas or liquid molecules from one place to another! (Is this an example of a “free lunch” or perpetual motion?… imagine if our cars worked like this….) Need to understand concepts of : • Diffusion, • Osmosis, • Concentration gradient, • Net movement Diffusion • The random movement of molecules from an area of high concentration to an area of low concentration. • Down the concentration gradient. Conc. Grad Rate (speed) of diffusion depends on: fast Size of molecules Temperature State of matter Concentration gradient slow Osmosis • Net movement of water molecules from an area of high water concentration to an area of low water concentration, through a semi-permeable membrane. • Hypertonic – more solute and less water • Hypotonic – less solute and more water • Isotonic – same conc. of solute and water Think (don’t write) • Most marine fish die if transferred to freshwater. • When a drop of blood is mixed with distilled water, the blood cells burst. • Living plant tissues that had lost water become firm when supplied with water. Net movement The overall movement of all molecules of a substance back and forward along the concentration gradient. e.g. when oxygen or water molecules diffuse into a cell, some molecules move out at the same time. Overall more are moving inwards than outwards so the net movement is……….. Osmoregulation • The control of water in a cell. • Plasmolysis – – Water leaves cell and membrane shrinks from wall. – Cell becomes flaccid. – Full plasmolysis is irreversible. • Turgor – Water enters cell causing it to swell – Cell becomes turgid – Cell wall prevents rupture Active Transport Energy (ATP) is required for: Endocytosis (phago- and pino-), Exocytosis, Ion pumps • e.g. re-absorption of glucose by kidney, salt uptake by the roots of plants, amino acid uptake by fungi • The movement of substances from an area of low concentration to an area of high concentration. • Against the concentration gradient Conc. Grad Cytosis 1.Endocytosis • Movement in (enter) to a cell • Phagocytosis – cell eating – forms a food vacuole. • e.g. Lysosomes fuse to enable digestion. • Pinocytosis – cell drinking – forms a vesicle • e.g. Amoeba and white blood cells (WBC) – pseudopodia 2.Exocytosis • Movement out (exit) • Vesicles bud off from golgi apparatus or ER, vacuole fuses with cell membrane and expels contents • Secretions e.g. digestive enzymes released, glands Ion Pumps • Proteins in the cell membrane can actively accumulate specific ions on one side of the membrane to control the amount