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COMPARING CELLS 1: PROKARYOTES vs. EUKARYOTES http://schoolhouse1.fenn.org/dduane/science9th/lab_comparing_cells_1_prokaryotes_&_eukaryotes.htm INTRODUCTION In this lab, students use microscopes to compare and contrast prokaryotic cells (bacteria) and eukaryotic cells (Protists). OBJECTIVES: By the end of this exercise, you should be able to... explain how the cell is the basic unit for all living things. relate characteristics of living things to cell structure. diagram prepared slides of prokaryotic cells (bacteria) and label the cell wall, cell membrane, and cytoplasm. Identify the shape of the species of bacteria. measure the length and width of bacteria cells in micrometers. diagram eukaryotic cells (Protists) and label the cell membrane, cytoplasm, nucleus, and any other organelles that are visible. measure the diameter of Protist cells in micrometers. compare and contrast Prokaryotic (bacteria) cells with eukaryotic (Protist) cells. PROCEDURE PART A: EUKARYOTIC CELLS (PROTISTS) 1. Acquire a prepared slide from the box labeled "Single Celled Eukaryotes (Protists)". 2. Label the species of Protist. 3. Focus the slide under the low power objective and move the slide to find good examples of these cells. Draw and diagram the cells. Label the cell membrane, cytoplasm, nucleus, and any other organelles that are visible. 4. Focus under medium power and diagram. 5. Focus under high power and diagram. PROCEDURE PART B: PROKARYOTIC CELLS (BACTERIA) 1. Acquire a prepared slide from the box labeled "Single Celled Prokaryotes (Bacteria)". 2. Label the species of Bacteria in your lab notebook. 3. Focus the slide under the low power objective and move the slide to find good examples of these cells. Diagram the cells in your lab notebook. 4. Focus under medium power and diagram. 5. Focus under high power and diagram. Label the cell membrane, (cell wall), and cytoplasm. Questions: Prokaryotic and Eukaryotic Cells Answer the following questions and statements using complete sentences. 1. Explain the function of the nucleus in eukaryotic cells and tell how prokaryotic cells carry out this function. 2. What structures do both prokaryotic and eukaryotic cells have? 3. What structures (organelles) do eukaryotic cells have that prokaryotic cells do not? 4. Compare the size and shape of both eukaryotic cells and prokaryotic cells. 5. Give examples of single celled organisms that are prokaryotic. 6. Give examples of single celled organisms that are eukaryotic. Diagrams In the Column 1, diagram a single bacterium (prokaryotic cell). Be sure to tell the total magnification, identify the cell, label the cell wall and cytoplasm, and indicate the size of the cell. In Column 2, diagram a single celled Protist (eukaryotic cell). Be sure to tell the total magnification, identify the cell, label the cell membrane, cytoplasm, nucleus, and other organelles, and indicate the size of the cell. 1. Bacterium (Prokaryotic cell) 2. Protist (Eukaryotic cell) Magnification: Magnification: Object: Object: