Download Flow cytometry measures the fluorescence or light diffraction of a

Cytometry : cell sorting & analysis
Platforms
Olivier Lantz
Platform Manager
[email protected]
Tel: +33 1 44 32 42 18
Flow cytometry measures the fluorescence or light
diffraction of a large number of particles at high speed,
such as cells, beads, bacteria, yeast, or organelles. At
Institut Curie, flow cytometry is used mainly to quantify
multiple markers on cells, with the option of
simultaneously sorting multiple sub-populations of
interest.
The primary advantage of flow cytometry is how quickly it produces data for a very large
number of cells, allowing for complex and/or rare sub-populations of cells to be analyzed and
sorted so that they can then be cultured or analyzed with molecular biology tools.
The cells in suspension may be simultaneously marked with 11 fluorochromes, each identifying a
molecule of interest. The marked cells flow past a laser and, for each cell, the fluorescence
intensity is quantified for each fluorochrome.
Fluorescent probes can be used to detect various
parameters such as membrane potential, pH, or cell cycle (proliferation, apoptosis, etc).
The flow cytometry platform performs cell sorting, and also trains people to independently use
high-speed multiparameter cytometers and the supporting software to acquire and analyze data.
Functions
cell sorting
training users on equipment and software
support for data acquisition and analysis
advice on cell preparation
results interpretation
INSTITUT CURIE, 20 rue d’Ulm, 75248 Paris Cedex 05, France | 1
Cytometry : cell sorting & analysis
Platforms
support for preparing manuscripts, i.e. figures, documents, and methods
Institut Curie’s main flow cytometry facilities, which are shared between the Hospital and the
Research Centre, are located in Laboratoire Laetitia at 26 Rue d’Ulm, Paris.
Equipment
2 analyzers: one FACSort (4 colors) and one LSRII (11 colors)
2 sorters: one FACSVantage (8 colors with UV) and one FACSAria (10 colors).
Contact and authorization to use the equipment
Analysis
The analyzers are self-service. Several other self-service cytometers are available at other
Institut Curie locations, in both Paris and Orsay.
To use the self-service cytometers at the platform in Laboratoire Laetitia:
Register at the laboratory,
Call to schedule an appointment at +33 1 56 24 58 01 (office) or +33 1 56 24 58 02
(laboratory)
Training is required to use the self-service equipment without supervision. Theoretical training
courses are given twice a year, and practical training is given once a month. All new users must
contact the platform managers for their first independent session, or if they would like
assistance from an engineer.
Sorting
Schedule an appointment with the managers:
Zofi[email protected] , [email protected] or [email protected]
+33 1 56 24 58 01 (office) or +33 1 56 24 58 02 (laboratory).
Available in the Clinical Immunology unit (4th floor: 26 Rue d’Ulm):
One self-service AutoMACS magnetic bead-based cell sorter
One Luminex analyzer, for quantifying multiple analyses of very small volumes in a
homogeneous phase, with an ELISA fluorescent bead system.
INSTITUT CURIE, 20 rue d’Ulm, 75248 Paris Cedex 05, France | 2