Download Flow Cytometry and Cell Sorting Facility

Program Director/Principal Investigator (Last, First, Middle):
RESOURCES
Follow the 398 application instructions in Part I, 4.7 Resources.
Flow Cytometry and Cell Sorting Facility
The Flow Cytometry and Cell Sorting Resource Laboratory is currently recognized as one of the
largest and most comprehensive flow cytometry laboratories in the US. In 2014 it was designated a
laboratory of exceptional merit by the National Cancer Institute. The resource provides a broad
array of instrumentation, support, education and consultation. A wide variety of cell sorting and
analytical applications are supported (up to 20 parameters) are offered. Currently the facility offers 6
cell sorters (4 BD Biosciences FACS Aria II, 1 BD Biosciences Influx, 1 BD Biosciences FACS
Jazz) and 15 analytical cytometers capable of up to 20 parameter analysis (BD LSRFortessa, BD
LSR II, BD FACSCanto, BDFACSCalibur, BD Accuri, Amnis ImageStream Imaging Flow
Cytometer). These instruments are located in satellite facilities throughout the University to
enhance access to user groups and are available for use, both sorters and analyzers, 24 hours per
day, 7 days a week. A very active training and consultation program is in place to support these
implementation of the technology in research programs. The Scientific Director, Dr. Jonni Moore
(Co-I), and the Technical Director, each have over 25 years of experience in the field of cytomics.
The Path Bioresource Computational Biology and Research IT facility, a division of the Flow
Cytometry Shared Resource, provides additional computer and database resources for advanced
computational analysis of flow cytometric data. The facility maintains a dedicated high-end Linux
compute server with 8 Intel cores, 32 GBytes of memory and a 12 TByte redundant RAID-5 storage
area network. The compute server is used to perform automated analysis of flow cytometric data,
including cytometric fingerprinting. It houses a MySQL database instance, where the raw cytometry
data are indexed with sample and patient metadata to facilitate database-driven computational
analysis. All of above resources are housed in a Tier 3 secure datacenter facility located at 3401
Market St, Philadelphia and operated by the University of Pennsylvania. In addition to the compute
server and related equipment the facility possesses several Linux, Mac and PC workstations. Raw
data and analysis results at Path Bioresource are backed up nightly. Laptop computers that connect
to the servers are password protected and their hard drives are encrypted.
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PHS 398 (Rev. 08/12 Approved Through 8/31/2015)
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OMB No. 0925-0001
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