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Transcript
BIT 495/595: Cellular Signaling Techniques Overview: • Par9cipants will be introduced to a variety of methods for studying cellular signaling processes including theory, applica9ons and limita9ons. • Students will use select techniques in the laboratory to examine the effects of a chemotherapeu9c agent on cancer cell signaling and survival. Lectures: 1. Introduc9on to cell signaling 2. Drug receptor interac9ons, dose-‐response rela9onships 3. Protein gel electrophoresis (1D and 2D) 4. Fluorescence microscopy (confocal, TIRF, PALM) 5. Flow cytometry 6. Immunoprecipita9on Labs: 1. Dose-‐Response and EC50 Determina9on 2 weeks 2. Nuclear and Cytoplasmic Frac9ona9on 1 week 3. Western Blot 1 week 4. Fluorescence Microscopy 1 week 5. Flow Cytometry and Analysis 3 weeks
