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Synthetic Genetic Array (SGA)
Platforms
Petra Kaferle
Platform Manager
[email protected]
Activity
Synthetic Genetic Array (SGA) is a high throughput approach allowing the systematic generation
of double mutants. Using a query strain, carrying a mutation of interest that is then crossed with
a library of mutants, one can obtain double mutants and score for synthetic interactions. Such
screens have been used extensively over the years to decipher the complexities of genetic
networks and pathways in yeast. A fully equipped laboratory dedicated to SGA technique has
been established at Institut Curie in 2012 and is available to all interested yeast and non-yeast
research groups.
Aims
Identifying genetic interactions between genes involved in individual pathways
Studying gene or protein functions
Elucidating mechanism of action of drugs
Networking
Manolis Papamichos-Chronakis, Institut Curie, Paris, France
Angela Taddei, Institut Curie, Paris, France
Antonin Morillon, Institut Curie, Paris, France
Stéphane Marcand, CEA, Fontenay-Aux-Roses
Philippe Pasero, IGH, Montpellier, France
Jorrit Enserink, Rikshospitalet University Hospital, Oslo, Norway
Services
Agar-based assays:
chemical genomics
synthetic genetic array
E-MAP
INSTITUT CURIE, 20 rue d’Ulm, 75248 Paris Cedex 05, France | 1
Synthetic Genetic Array (SGA)
Platforms
…
Liquid-based assays:
growth curves
colorimetric assays
Equipment
Robotic manipulator BM3-BC (S&P Robotics, Inc.):
96, 384 and 1536-format replicating heads
96-format re-arraying head
capacity 48 plates
integrated camera
Spectrometer Eon + plate handler BioStacker3 (BioTek):
200 to 999 nm wavelength range
Different modes of shaking
Incubation chamber
S.cerevisiae collections:
Yeast Knockout Collection
Temperature-sensitive mutant collection
Decreased Abundance by mRNA Perturbation (DAmP) collection
Yeast Synthetic Histone Collection
GFP-tagged ORFs
VN-Fusion Library
Training
SGA platform does not offer any training.
INSTITUT CURIE, 20 rue d’Ulm, 75248 Paris Cedex 05, France | 2