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Synthetic Genetic Array (SGA) Platforms Petra Kaferle Platform Manager [email protected] Activity Synthetic Genetic Array (SGA) is a high throughput approach allowing the systematic generation of double mutants. Using a query strain, carrying a mutation of interest that is then crossed with a library of mutants, one can obtain double mutants and score for synthetic interactions. Such screens have been used extensively over the years to decipher the complexities of genetic networks and pathways in yeast. A fully equipped laboratory dedicated to SGA technique has been established at Institut Curie in 2012 and is available to all interested yeast and non-yeast research groups. Aims Identifying genetic interactions between genes involved in individual pathways Studying gene or protein functions Elucidating mechanism of action of drugs Networking Manolis Papamichos-Chronakis, Institut Curie, Paris, France Angela Taddei, Institut Curie, Paris, France Antonin Morillon, Institut Curie, Paris, France Stéphane Marcand, CEA, Fontenay-Aux-Roses Philippe Pasero, IGH, Montpellier, France Jorrit Enserink, Rikshospitalet University Hospital, Oslo, Norway Services Agar-based assays: chemical genomics synthetic genetic array E-MAP INSTITUT CURIE, 20 rue d’Ulm, 75248 Paris Cedex 05, France | 1 Synthetic Genetic Array (SGA) Platforms … Liquid-based assays: growth curves colorimetric assays Equipment Robotic manipulator BM3-BC (S&P Robotics, Inc.): 96, 384 and 1536-format replicating heads 96-format re-arraying head capacity 48 plates integrated camera Spectrometer Eon + plate handler BioStacker3 (BioTek): 200 to 999 nm wavelength range Different modes of shaking Incubation chamber S.cerevisiae collections: Yeast Knockout Collection Temperature-sensitive mutant collection Decreased Abundance by mRNA Perturbation (DAmP) collection Yeast Synthetic Histone Collection GFP-tagged ORFs VN-Fusion Library Training SGA platform does not offer any training. INSTITUT CURIE, 20 rue d’Ulm, 75248 Paris Cedex 05, France | 2
