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Danyal Education Movement of Substances Notes 1 UNIT 2 M()UEMENT ()F SUBSIANCES Learning Objectives: gl g g Definitionofdffision Definition of osmosis Osmotic changes in cells may have drastic consequences. . Plant cells may undergo plasmolysis or become turgid . Animal cells may undergo crenation or haemolysis Definition ofwater potential Defnition and examples of active transport in living cells . . . uc ati o DIFFUSION n g g Diffusion is the movement of molecules from a region of their higher concentration to a region of their lower concentration, down a concentration gradient. lt is a passive kind of transport because energy, in a form of ATB is not required for the movement of molecules from one place to another. When the molecules have been spread evenly between the two regions, the concentration gradient is no longer present. As a result, there will be no net movement of molecules between these regions anymore. o Ed after some time direction of movement of particles o o o o^o oo eo oa oa ooo o particles are evenly distributed lmportant role of ditfusion in living organisms . . al . Diffusion enables living organisms, unicellular or multicellular, to survive by allowing the exchange of nutrients, gases and waste substances between them and the surroundings. Unicellular organisms such as amoeba and paramecium take in oxygen and excrete waste substances through simple diffusion. Plants and animals require energy to survive. The living cells use up oxygen during aerobic ny . respiration. This process causes the concentration of oxygen in the cells to decrease. As a result, oxygen molecules are able to diffuse into the cells from the surroundings down their concentration gradient. The cells can obtain fresh oxygen molecules to carry out this important process again. Diffusion also plays an important role in the uptake of nutrients in animals and plants. During the process of digestion, soluble digested substances diffuse into the bloodstream from the lumen of the small intestine due to the presence of concentration gradient of the nutrients between these two regions. Da '/ '/ ' Minerals and nutrients are absorbed into the root hairs through diffusion when the concentration of these substances are higher in the surroundings than in the root hair cells. Diffusion allows living organisms to remove the metabolic waste substances, such as ammonia, excess water and mineral salts, in the tissues. They diffuse out of the tissues into the bloodstream and are transported to the respective excretory organs. (- o O 0LM Pt* ltd BIOLOGY O Level Conceprual Learning ||/ O I REVISION 7. (a) Define diffusion Explain what is meant by concentration gradient of a substance. Ed uc ati o n (b) Answer True or False for each of the following statements given below. 2 (a) Diffusion of a substance is affected by the presence of another type of substance (b) The particles of a substance stop moving between two regions when equilibrium is reached. (c) The steeper the concentration gradient for a substance, the faster the rate of diffusion. (d) A permeable membrane restricts the movement of certain substances across it. (e) Complex substances cannot pass through the cell membrane due to its size (f) The gaseous exchange taking place in plant cells and animal cells involves diffusion only. 0sM0srs . ny membrane. al Osmosis is the movement of water molecules from a region of their higher concentration (higher water potential) to a region of their lower concentration (lower water potential), through a partially permeable Da higher solute concentration (lower water concentration) I lower solute concentration (higher water concentration) after some time r solute molecules i.i:i: water molecules i Oartially permeable membrane : equal solute concentration level of water on right side has decreased and that on left side has increased a 18 osmosis is a passive transport as well because it does not consume energy. o GIM Ple ltd WATER POTENTIAL . . Water potential is the measure of the tendency of water to move from one place to another. A dilute solution has more water molecules per unit volume than a concentrated solution. Hence it has higher water potential. Water always moves from an area of higher water potential to a lower one. . . Cell membrane is a partially permeable membrane that allows some substances to pass through but not others. The concentration of dissolved substances is usually higher inside the cell than outside. This allows water to enter by osmosis. ati . on Application to living cells water enters! lower concentration of dissolved su outside the cell -+ higher water outside the cell uc concentration of dissolved substances inside the cell -+ thus lower water potential inside the cell Leaf cell (An example of plant cells) Effect in concentrated solution Etfect in dilute solution Plasmolysis occurs when cells Turgor pressure results when cells are placed are placed in a dilute solution. Water enters the cell Vacuole increases in size Cellulose cell wall prevents over in a concentrated solution. Water leaves the cell Vacuole decreases in size Cytoplasm shrinks away from the cell wall al Type of cells Ed tflect ol osmotic chonges in cells tn concentrated solution Da ny + expansion of the cell membrane by exerting an opposing pressure. a When cell is fully turgid, further entry of water is prevented. in dilute solution + cell becomes plasmolysed cell becomes turgid * J o o 6lM Pte Ltd BIOLOGY O Level Conceprual Learning ||| Haemolysis occurs when cells are placed in a dilute solution. solution. Water leaves the cell Membrane of the cell forms little Water enters the cell spikes of the cell membrane Cell bursts . Cell shrinks and dehyd rated . Cell dies Red blood cell (An example of animal cells) tn concentrated solution + becomes Animal cells do not have cell walls to prevent over expansion Cell dies n . . Crenation occurs when cells are placed in a concentration in dilute solution ffi haemolysis L. REVISION 2 uc O Diffusion is a result of the random movement of (a) from a region of their (b)........ r\l \Y \\J cell undergoes cell undergoes crenation t ati o . The net movement is ...... concentration to a region of their (c) Ed concentration, down a concentration gradient. The particles continue to (d)....... even when they are evenly spread throughout. Osmosis is the diffusion of (e)..... ......... molecules. When a (0....... permeable membrane like a cell membrane separates two solutions of different concentrations, a concentration gradient is set up. Water molecules move in both directions, but the (g). ............... (a) Define osmosis. 3. A dilute solution and a concentrated solution has different water potential. what is meant by water potential? Da (b) ny 2 al movement of water is from a dilute solution to a concentrated solution. The diagram below shows a beaker containing two different liquids. They are separated by a semipermeable membrane. The beaker is left undisturbed for one hour. A B semi-permeab membrane 10% starch solution 20 distilled water O GIU Ple ltd (a) (i) Explain your answer. Da ny al Ed uc ati on (ii) What would happen to the liquid levels in regions A and B after one hour? (b) (i) (ii) 4. starch solution is replaced by lOo/o glucose solution, what would you expect to happen to the liquid levels in regions A and B? lI 7Oo/o Explain your answer. The diagram below shows how osmosis affects plant cells when they are placed in different concentrations of solution. Diagram after one hour in solution Y after one hour in solution X + --f Which region has higher water potential Changes of cell after one hour Explanation C x o BIOLOGY 0 Level Conceptual Learning I lt 5. The diagram below shows how osmosis affects animal cells when they are placed in different concentrations of solution. Diagram after one hour in solution Q after one hour in solution P + € ffi Which region n has higher uc ati o water potential Changes of cell after one Explanation An animal cell and a plant cell were both placed in distilled water. After one hour, they were examined again under an optical microscope. Only fragments of cell membrane were observed in animal cell while the plant cell has increased in size. ny al 6 Ed hour Da Explain why the results are different in both types of cells. ACTIVE TRANSPORT . Active transport is the movement of substances across a cell membrane against gradient, using energy from respiration. a concentration Examples ,/ ./ 22 The cells lining the gut take up glucose molecules into the blood, which already contains a relatively high amount of glucose. Mineral ions are absorbed by the root hair cells even though the concentration is much lower in the surrounding soil particles than in the root hair cells. o 6lM Pre lrd glucose molecules or @REVISI0l'l 1,. (a) 5 mineral ions enter! (dissolved substances enter the cell against their concentration gradient) higher concentration of dissolved substances such as glucose or mineral ions inside the cell al Ed uc ati on lower concentration of dissolved substances such as glucose or mineral ions outside the cell Define active transport. (b) Name the cell organelle that will be present in a greater amount in a cell if the cell carries out active transport constantly. Explain your answer. 2. State two examples of active transport taking place in living organisms. . ' The surface area to volume ratio is the amount of surface area per unit volume of an object. ln living organisms, it is an important factor that affects the rate of diffusion of substances across the cell membrane. Nutrients, oxygen and waste substances need to diffuse through the cell membrane in order to enter or leave the cells. Cells with a large surface area to volume ratio enable nutrients and oxygen to diffuse into the cell quickly and allow waste to diffuse out of the cell quickly. lf the cells have a small surface area to volume ratio, the nutrients and oxygen will take longer time to diffuse into the cell. As a result, the cell would not be able to survive. Da ' ny SURFACE AREA TO UOLUME RATIO ' Even though larger cells have more surface area than smaller ones, they have less surface area for every unit volume of protoplasm. Hence their rate of intake of nutrients per unit volume is slower than smaller cells. (- o O SLM Pte lld BIOLOGY O LevelConceprual Learning | !$ Calculation of surface area to volume ratio 2 1, L 1 Height, H (m) 1 Width, W (m) 1 1 Surface area, 6 Volume,LxHxW(ms) Surface a rea/Vol ume ratio 2 3 2 3 4 9 24 54 27 8 1 (6/1):6, 1 (2418)=3:l $4127) :2: t The smaller the size of an object, the greater the surface area to volume ratio, hence the faster the movement of substances in and out of the object. Conclusion @REVlsloN J Ed uc [Note: There are a total of 6 sides in each cube.l J 3 2 Surface area of one side, 6xLxH(m'z) 2 2 Length, L (m) LxHh2) 3 L ati on Cube 4 ln living organisms, the exchanges of nutrients, gases and wastes occur through the surface area of the cells, which is the cell membrane. The surface area is a critical interface between the cell and its environment. The greater the surface area to volume ratio, the faster the rate of diffusion of a substance for a given concentration gradient. The diagram below shows how the surface area to volume ratio can be calculated and how exactly it changes with changing size. 2cm 4cm 3cm Da ny al L. Surface area Volume Surface area to volume ratio (4nr3)13 respective ly. Take [The equations for: surface area = 4nr2 and volume = 24 r as 3.14.1 O GIM Ple Ltd (a) Calculate the surface area, volume and surface area to volume ratio for each sphere (c) The sphere can be used to represent the three-dimensional structure of a cell. Why does the surface area to volume ratio decreases when a cell grows? For a single-celled organism or a cell in a multicellular organism's body, exchange of materials often occurs through the process of diffusion which is a passive process. Why is diffusion said to be a passive process? n (b) (ii) Why is the surface area to volume ratio important for the survival of a cell? The diagram below shows the outline of a root hair cell Ed u 2. ca tio (i) highly elongated and protrusion Explain why the root hair cell is highly elongated and protrudes from the root surface al (a) (b) State two more examples of cells in the living organisms that have similar adaptation to perform Da ny their functions more efficiently. (- o= o 6LM Pte Ltd BIOLOGY 0 LevelConceprual Learning I lg