Download Biochemistry Key Answers

BIOCHEMISTRY-I
April-2001
1. Describe the chemistry, sources, daily requirement and biochemical role of cholecalciferol.
2. Write short notes on:
(a) Absorption of lipids. (b) Line weaver – Bunk plot. (c) Name the four protolipid complexes of
respiratory chain, with their components. (d) Name three function tests that suffice in the
differential diagnosis of jaundice. Explain giving normal values. (e) Interpretation of
metabolism via TCA cycle. (f) Define ketosis. Name two conditions where it occurs. (g)
Calculate your daily carlorie requirement. (h) Structure of cell membrane. (i) Heme
catabolism. (j) Disorders (name) of malnutrition.
April-2001
1. Explain the effects of different factors on rates of enzyme catalyzed reactions.
2. Write short notes on:
(a) Sources of acetyl CoA. (b) Anaplerotic reactions. (c) Chemiosmotic hypothesis. (d) Acute
intermittent porphyria.
3. How are lipoproteins classified? What are their functions? Describe the metabolism of low
density lipoproteins.
4. Write short notes on.
(a) Write short notes on. (b) Vitamin K. (c) Von Gierbe’s disease. (d) Classification of enzymes.
March-2002
1. Define enzyme. Write about factors regulating enzymatic activity. Name four clinically
important enzymes. Mention their importance.
2. Short notes on:
(a) Lipoproteins. (b) Active from of Vit. D and its Biochemical role. (d) Inhibitors of respiratory
chain.
3. What is normal Glucose value? Write in detail about its regulation.
4. Short notes on:
(a) Essential fatty acids. (b) Tests to identify ketone bodies in urine. (c) Abnormal hemoglobin.
(d) Specific Dyanamic Action ( SDA)
March-2002
1. Give an account of Embden-Meyerhoffs pathway in carbohydrate metabolism. Describe the
substrate level of phosphorylation and its importance in this pathway.
2. Write short notes on:
(a) Mitochondria. (b) Km value and its significance. (c) Uncouples of oxidative
phosphorylation. (d) Specific dynamic action of food stuffs.
3. Name the importance vitamins which require for the proper functioning of the nerve. Describe
the sources, Biochemical functions, daily requirements and deficiency manifestations of any
one of them.
4. Write short notes on:
(a) Essential Fatty acids. (b) Galactocemia. (c) Haemoglobinopathies. (d) Vanden Bergh’s
reaction and its interpretation.
September-2002
1. What is urea? Describe the steps of urea synthesis. What is the significance of urea cycle?
Name the disorders of urea cycle.
2. Write short notes on:
(a) Structure and functions of human insulin.(b) Synthesis and functions of active form of vit.
D. (c) Biological value and its applications.
3. Discuss in detail glycogen metabolism. Mention glycogen storage disorders.
4. Write short notes on:
(a) Fatty liver and lipotropic factors. (b) Creatinine clearance test. (c) Explain the
chemiosmotic theory of oxidative phoshorylation. (d) Discuss the manifestations,
molecular bases, and laboratory diagnosis of sickle cell disease.
August-2004
I.
II.
Essay:
(1) Describe TCA (Citric Acid Cycle) cycle and its inhibitors and energetics. Add a note
on the Amphibolic role of TCA cycle. How is TCA cycle regulated?
(2) Discuss cholesterol synthesis and its regulation. Mention the various substance
obtaines from cholesterol.
Write short notes on:
(1) Phosholipids. (2) Biotin. (3) Isoenzymes. (4) Protein energy Malnutrition. (5)
Obstructive Jaundice. (6) Uncouplers of oxidative phosphorrylation. (7) Absorption
of Carbohydrates from the intestines. (8) Structure of Hemoglobin. (9) Bile salts.
(10) Vitamin E.
February -2005
I.
II.
(1) What is enzyme inhibition? Describe different kinds of enzyme inhibitions with
examples.
(2) Describe in detail the synthesis and utilization of ketone bodies. Name any three
conditions of increased production of ketone bodies and mention Laboratory
evaluation of ketoacidosis.
Write short notes on:
(a) Lipoproteins and their functions. (b) Significance of HMP- shunt pathway. (c)
Synthesis and conjugation of bilirubin. (d) Fate of acetyl CoA (e) Functions and
deficiency symptoms of Vit. A. (f) Isoenzymes and their diagnostic importance. (g)
Galactosemia. (h) Cyclic AMP. (i) Recommended daily dietary allowance. (j)
Essential fatty acids.
August-2005
I.
II.
Essay:
(1) What are the sources, properties, functions deficiency manifestations daily
recommended allowances of Ascorbic acid.
(2) What is the normal fasting and post prandial blood glucose level? Explain how
normal glucose level is maintained. Add a note on the disruption of hormonal
regulation of blood glucose.
Short notes on:
(a) Allosteric enzymes and its feedback regulation. (b) Phospholipids and their
clinical importance. (c) Inhibitors of electron transport chain. (e) Diagnostic value
of Isoenzymes. (f) BMR. (g) Fatty acid synthasis. (h) Heme degradation. (i)
Eicosanoids (j) LDL Metabolism.
February-2006
I.
II.
Essay:
1. Describe the process of Glycogen synthesis and Glycogenolysis.
2. What is normal serum Cholesterol level? Describe the process of synthesis of
Cholesterol.
Write short notes:
(a) Mutarotation. (b) Factors regulating the enzyme action. (c) Anaplerotic reactions.
(d) Ehat are ketone bodies? Describe the formation of ketone bodies.(e)
Chemiosmotic theory. (f) Justify the statement that Vitamane D is an hormone.
(g) Thiamine. (h) Vitamine B 12. (i) Acute intermittent porphyria. (j) Physiologic
jaundice.
August-2006
I.
II.
Essay:
1. Write detail about β – Oxidation of fatty acid under the following heading:
(a) Definition (b) Site (c) Stops and (d) Energetics.
2. Discuss citric acid cycle under following heading:
(a) Location (b) Reaction and (c) Energetics.
3. Write an essay on Chemistry, functions, deficiency manifestations and
hypervitaminosis state of Vitamin A.
Write Short notes on:
(a) Define and classify polysacchaides with examples. (b) Define and classify
enzymes with examples. (c) Protein energy malnutrition. (d) Acute intermittent
porphyria. (e) Role of cytochromes in electron transport chain. (f) Comparison
between prokaryotic and eukaryotic cells.
February-2007
I.
Essay questions:
1. Write are iso enzymes? Write about different iso enzymes and their clinical
importance.
2. Describe the chemistry, source, daily requirement, biochemical functions and
deficiency manifestation of folic acid
II.
Short notes on:
(a) Phospho lipids. (b) Congenital hyper Bilirubinemias. (c) Formation of ketone and
their clinical significance. (d) Basal metabolic rate. (e) HMP shunt pathway. (f)
Oxidative phosphorylation.
August-2007
1. Define biological oxidation. Mention the components and organization of
electron transport chain. Describe in detail the mechanism of ATP synthesis.
Add a note on uncouplers.
2. Describe the chemistry, sources, and RDA and biochemical functions of
thiamine. Add a note on beri beri.
3. Short answers:
(a) Poly unsaturated fatty acids. (b) Balanced diet. (c) Glycosylated
hemoglobin. (d) Chondroitin sulphate. (e) Synthesis and utilization of
ketone bodies. (f) Rapoport – Leubering cycle. (g) Folate trap. (h)
Glycosides. (i) Apolipoproteins and their significance. (j) Renal glycosuria.
February -2008
Essay Questions:
1. Describe the site, process of β oxidation of fatty acid and add a note on role of
CARNITIN. Write the emergetic when palmitic acid is oxidized.
2. Describe the synthesis of HEME. What is PORPHRIA? Classify the different
types of porphyrias. Give the enzyme defact and biochemical findings.
3. Write short notes on:
(a) Biotin. (b) Enzymes in myocardial infarction. (c) Protein energy
malnutrition disease. (d) Oxidative phoshorylation. (e) Structure of cell
membrance. (f) How glucose is absorbed from small intestine? (g)
Catabolism of cholesterol. (h) Wald’s visual cycle. (i) Glycogen storage
disease. (j) Fibre diet.
August-2008
I.
II.
III.
Essay questions:
1. Explain the steps of beta oxidation of Palmitic acid. Add a note on Energetics.
2. What is Gluconeogensis? Describe the pathway involved in gluconeogensis.
Add a note on regulation of Gluconeogenesis.
Write Short notes on:
1) Functions of Vit C. 2) Digestion and absorption of lipids. 3) Membrane.4)
Isoenzymes. 5) Structure of cell membrane. 6) Define BMR. What are the
factors that can affect BMR? 7) Define Oxidative phosphorylation.
Explain chemiosmotic theory. 8) Galactosemia. 9) Ketogenesis. 10)
Glucose tolerance Test.
Short Answer Questions:
1) Name the Essential fatty acides.2) Significance of HMP shunt pathway.
3) Benedicts test. 4) Inhibitors of citric Acid cycle. 5) Chloride shift.6)
Functions of calcium. 7) Lipotropic factors. 8) Normal blood levels of 1.
Cholesterol. 2. Bilirubin. 3. Sodium, 4. Potassium. 9) Phospholipids 10)
Flurosis.
February-2009
I.
II.
III.
Essay Questions:
1. Sources& fate of acety 1 CoA , Explain the denovo synthesis of cholesterol&
its regulation.
Write Short notes on:
1. Write a note an chemiosmotic theory. 2. Active transport. 3. Uronic acid
pathway. 4. Insulin. 5. Wald’s visual cycle.6. Collagen. 7.
Glycosaminoglycons (GAGS). 8. Chromatography. 9. Levels of
organization of proteins. 10. Calcium Homeostasis.
Short Answer Questions:
1. Key enzymes of glycolysis. 2. Fatty liver. 3. Lipid peroxidation. 4.
Zymogens. 5. BMR.
6. Normal levels of:
i)
B.U.N
ii) Fasting Serum Glucose
iii)
LDH
iv) ALT
7. t: RNA . 8. Vitamin K. 9. Limiting amino acid. 10. Isoenzymes.
August-2009
I.
Essay Questions:
1. What are Porphyias? Classify different types of porphyrias and give the
enzyme defect and biochemical finding.
2. What is oxidative phosphorylation? Discuss the steps of the same and
mention its significance.
Write Short notes on:
1. Classify RNA and explain the functions. 2. Hyper uricemia. 3. Renal
glycosuria. 4. Cardiac troponin. 5. Structure of cholesterol and its
importance in the body. 6. Beri Beri. 7. Enzyme poisons. 8. Flurosis. 9.
What is protein energy malnutrition (PEM)? What are the types of
PEM? Write the importance features. 10. Functions of Vitamin C.
Short Answer Questions:
1. Effect of Temperature on enzyme activity. 2. Define epimers. Name
two epimers. 3. Phosphotidy 1 inositol importance. 4. Biochemical
functions of selenium. 5. Benedicts test. 6. Ribose and deoxy ribose. 8.
Bence Jones proteins. 9. Bile salts. 10. Cori cycle.
II.
III.
February -2010
I.
II.
III.
Essay Questions:
1. Define enzymes. Classify Enzymes with suitable examples. Explain the
concept of active site of enzymes.
2. Describe the steps of HMP shunt pathway. What is its significance? How
is it regulated?
Write short notes on:
1. Nutritional importance of proteins. 2. Describe the requirement,
sources, metabolic functions and deficiency manifestations of folic acid.
3. Explain with a neat labeled diagram of fluid mosaic model of
biological membrane. 4. Total parenteral nutrition and its importance.
5. t- RNA. 6. Explain the metabolism and functions of HDL. 7. What are
glycoproteins? Give three examples and its importance. 8.
Chemiosmotic theory. 9. Rapa port laboring shunt pathway and its
significance. 10. What are Nucleotides? Name any three biologically
Important nucleotides and their importance.
Short Answer Questions:
1. Why sucrose is called a non reducing disaccharide?2. Name the
essential fatty acids. 3. Name any four biologically important
compounds derived from cholesterol. 4. What are Phospholipids? Give
two examples.5. Name the essential aminoacids.6. Mention any two
biological function of albumin. 7. Name the amino acids required for
purine biosynthesis. 8. Sickle cell hemoglobin. 9. Specific dynamic
action. 10. Write the principle and significance of biuret test.
August-2010
I.
II.
III.
Essay Questions:
1. Describe the Citric acid cycle. How is it regulated? What is its
amphibolic role?
2. Describe the chemistry, absorption, functions, and deficiency
manifestations of Vitamin A.
Write short notes on:
1. Inhibitors of Electron Transport Chain.2. Transport of Bilirubin. 3.
Vitamin E. 4. Substrate level Phosphorelation. 5. Gluconeogenesis. 6.
Regulation of enzyme activity. 7. Abnormal hemoglobin’s. 8. Digestion
and absorption of Triacylglycerols. 9. Biochemical importance of
derivatives of Cholesterol. 10. Significance and disorders of Pentose
Phosphate pathway.
Short Answer Question:
1. Mutarotation.2. Subcellular organelles. 3. Basal metabolic rate. 5.
Essential amino acids. 6. Causes of fatty liver. 7. Renal glycosuria. 8.
Role of HDL as scavenger of Cholesterol. 9. FIGLU. 10. Dietary fibers.
February-2011
I.
II.
III.
Essay Questions:
1. Describe the chemistry, sources, daily requirement, biochemical
functions and deficiency manifestations of Vitamin-B12.
2. Describe how cholesterol is synthesized in our body. What are the
products formed from Cholesterol?
Write Short notes on:
1. Active form of Vitamin D and its biochemical role. 2. Catabolism of
Hemoglobin. 3. Protein energy malnutrition 4. Ketogenesis. 5. Fatty
acid synthase complex. 6. Glycogen Metabolism. 7. Enzyme inhibition.
8. Glycosylated hemoglobin. 9. Oxidation phosphorylation. 10.
Regulation of blood glucose.
Short Answer Questions:
1. Zymogen.2. Name two zinc containing enzymes. 3. Ferritin. 4. Define
Km. 5. Functions of selenium. 6. What are cytochromes? 7. Brown
adipose tissue. 8. Lactose intolerance. 9. Define respiratory quotient.
10. Functions of Vitamin K.
August-2011
I.
II.
III.
Essay questions:
1. Describe in detail TCA cycle and the energetic of the same. Justify why
TCA cycle s called an amphibolic cycle.
2. Describe in detail the components and chemiosmotic theory of
electron transport chain.
Write short notes on:
1. Role of Niacin as Coenzyme.2. Classification of hyperlipidemias& their
clinical importance. 3. Sphingolipidoses. 4. Biochemical role of Vitamin
C. 5. Cori’s cycle and glucose Alanine cycle. 6. High Density Lipoprotein
Cycle. 7. Glycogenolysis. 8. Isomerism in carbohydrates. 9. Balanced
Diet. 10. Fructose intolerance.
Short Answer Questions:
1. Markers for lysosomes and mitochondria. 2. Fluorosis. 3. Role of Apo
CII. 4. Define metalloenzymes with 2 examples. 5. Pulmonary
surfactant- Structure and clinical importance. 7. What is the function of
Lipoprotein lipase? 8. Structure of lecithin. 9. Net protein Utilization.
10. Chondroitin sulphate- Structure. 11. Double Reciprocal plot. 12.
Alkaline phosphatase as a diagnostic tool. 13. What are the different
forms of calcium in blood? 14. RDA and functions of Iodine. 15. Why
Arachidonic acid is not considered “purely” an essential fatty acid?
February-2012
I.
Elaborate on:
1. Describe the components and reactions of electron transport chain.
Add a note on its inhibitors.
2. Describe the dietary sources, daily requirements, biochemical
function and deficiency symptoms of vitamin C.
II.
Write notes on:
1. Balanced diet. 2. Causes of hypoglycaemia. 3. Allosteric inhabitation.4.
Obesity.5. Alkaptonuria. 6. Functions of mitochondria. 7. Glycosylated
haemoglobin. 8. Neo glucogenesis. 9. Thalessemias. 10. Puring
salvage path way.
Short Answers:
1. Markers of nucleus and mitochondria. 2. Name 2 tumour markers. 3.
Functions of phosphor lipids. 4. Name the essential fatty acids. 5.
Active forms of Thiamine and Riboflavin. 6. Name the ketone
bodies.7. Significance of rapaport- leubering cycle. 8. Name two
glycogen storage diseases. 9. Significance of HMP shunt. 10. Name the
III.
urea cycle disorder. 11. Name the urea cycle disorder. 12. Causes of
increased blood urea level. 13. Name the derivatives of tryptophan.
14. Fluorosis .15. Parameter for the assessment of nutritive value of
proteins