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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034 M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY FIRST SEMESTER – NOVEMBER 2007 AE 16 BT 1814 - BIOMOLECULES Date : 27/10/2007 Time : 1:00 - 4:00 Dept. No. Max. : 100 Marks PART - A Answer all the Questions I. Choose the best answer : 1. All the following sugar are interconvertible except a) Glucose b) galactose c) Mannose d) fructose 2. Purine metabolise to form a) urea b) ammonia c) uric acid (20 Marks) (5 x 1 = 5) d) all the above 3. Depot fat in mammalian cells principally comprises a) cholesterol b) cholesterol ester c) cerebroside d) Triacyl glycerol 4. Non protein part of any enzyme is called a) apoenzyme b) epienzyme c) coenzyme 5. Hemoglobin is a a) dimeric protein protein b) tetrameric protein d) holoenzyme c) trimeric protein d) monomeric II. State True or False, if False, state the reason 6. 7. 8. 9. 10. Xanthine oxidase oxidizes, hypoxanthine to xanthine. FAD is a nucleotide coenzyme Multiple forms of enzymes catalyzing the same reaction are isoenzymes. Glycogen is composed of β glycosidic bonds. Plasmalogens contain an enol ether linkage at C2 position. III. Complete the following 11. 12. 13. 14. 15. (5 x 1 = 5) (5 x 1 = 5) The α and β cyclic forms of D glucose are referred to as__________ The charged amino acid, which is electrically neutral, is called _________. _________ is the specific region on the enzyme at which substrate binds. _________ is the ring system present in cholesterol. Nitrogenous bases are conjugated to pentose sugars by_________ linkage. 1 IV. Answer the following, each within 50 words 16. 17. 18. 19. 20. (5 x 1 = 5) Why is sucrose called non-reducing sugar? Define Turnover number of an enzyme. Write the structure of d GTP What are essential fatty acids ? What are non protein amino acids ? PART - B Answer any 5 questions each within in 350 words only 21. 22. 23. 24. 25. 26. 27. 28. (5 x 8 = 40) Derive Michalis Menten equation and mention the significance of Km value. Explain the properties of fatty acids. Discuss the syntheis and degradation of triacylglycerol Discuss the biosynthesis of pyrimidine bases Explain the classification of amino acid with the structure. Write the structure for i) NAD+ ii) Lactose iii) Cholesterol Give an account on Mucopolysaccharides Write an account on Phospholipids PART - C Answer each of the following, within 1500 words 29. Discuss the secondary structure of proteins ? Mention the forces responsible for the maintenance of structure of protein OR Explain in detail the properties of monosaccharides 30. Write elaborately the denovo synthesis of fatty acid OR Describe the IUB system of classification of enzymes. (2 x 20 = 40) ******************* 2