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Thank-you for attending Biol120 Mock Final Exam, brought to you by the Bio 120 SSS Peer Mentors and the University Learning Centre! To help inform future programming and mentoring in Biology 120, we would like to gather attendance information. 1) Write your NSID on this sheet of paper 2) Remove it from the stapled bunch 3) Place it in the marked bins (by the doors or front of the room) on your way out Students who leave their name and NSID will be entered into a draw to win one of three $10 Starbucks gift cards. Winners will be contacted by email from a ULC staff member. Thanks again for attending and good luck! NSID: _______________________________ Questions and answers for the mock midterm will be posted after Wednesday Dec. 3, 2014 at: http://www.usask.ca/ulc/sss, as well as to your blackboard course. Name: Student Number: All students, from all sections, welcome! The Biology 120 Peer Mentors and the University Learning Centre Present: Biol120 Mock Final Examination Wednesday, December 3rd, 2014 Time: 6:30-7:30pm (60 mins) Location: HLTH 1150 Important note: This mock final exam was written by your Biology 120 Peer Mentors, not your professors! It is designed to help you test yourself on topics and concepts covered in class but should not be considered an exact preview of the final exam. You are required to remain in your seat for 30 minutes, but are free to leave the room after 30 minutes (7:00pm). No talking, please! No use of electronic devices (ie. cell phones, lap tops, etc.). This exam consists of 50 questions. Be sure to carefully read the questions and all possible answers. Choose the most correct answer. Only select one answer per question. You have 60 minutes to complete this exam. We encourage you to take note of your start and finish time, or to keep track of how many questions you were able to complete in the 60 minutes. This may help you to better judge your time for the final exam. Start Time: ________________ End Time: ________________ Questions completed at 60 mins: ___________ You are invited to return to the exam room ~7:45 for the Question and Answer period with your Peer Mentors. Questions and answers for the mock midterm will be posted after Wednesday Dec. 3, 2014 at: http://www.usask.ca/ulc/sss, as well as to your blackboard course. 1. What phase makes up 90% of the cell cycle? a) G1 b) S c) G2 d) Interphase 2. In phospholipids, the phosphate head is ____________, and the lipid tails are _____________. a) Hydrophobic, Hydrophobic b) Hydrophobic, Hydrophilic c) Hydrophilic, Hydrophobic d) Hydrophilic, Hydrophilic 3. In DNA replication, the ______________ grows ____________ away from the replication fork. a) Lagging strand, continuously b) Lagging strand, discontinuously c) Leading strand, continuously d) Leading strand, discontinuously 4. Co-dominance is: a) when the offspring have all the same phenotype b) when the activity of one allele is insufficient to compensate for the inactivity of another. c) When genes have multiple alleles d) When both alleles are equally active 5. After equilibrium is reached in the concentration of solutes inside and outside a cell, what will be the net movement of molecules? a) Into the cell b) Out of the cell c) In and out of the cell at equal rates d) The membrane doesn’t allow any molecular movement after equilibrium 6. During oxidative phosphorylation the Electron Transport Chain builds a proton gradient across_______________ in eukaryotes a) the cell membrane b) the inner mitochondrial membrane c) the outer mitochondrial membrane d) the nuclear membrane Questions and answers for the mock midterm will be posted after Wednesday Dec. 3, 2014 at: http://www.usask.ca/ulc/sss, as well as to your blackboard course. 7. What is the role of a kinetochore? a) It acts as the main microtubule organizing center in plant cells b) It attaches the chromosome to and walks along microtubules c) It condenses chromosomes d) It regulates cell division 8. Based on his experiments Mendel found he was able to predict that: a) Half of the offspring will have the same genotype as one of their parents. b) As gametes are formed half the gametes carry one allele, and the other half carry the other allele. c) The alleles of the genes that govern the two characters segregate independently, during formation of gametes. d) None of the above 9. Why is Drosophila a useful model organism for genetics experiments? a) It can be used to study human diseases b) It can reproduce rapidly c) It can produce many offspring in a short period of time d) All of the above 10. Which of the following statements describes eukaryotic mRNA? a) It must be spliced to cut out introns and seals together translatable exons b) It is translated by ribosomes and it is transcribed by an RNA polymerase prior to export from the nucleus c) It has a guanine cap on its 3’ end and a poly-A tail on its 5’ end d) It is a polymer of adenine, thymine, guanine, and cytosine bases 11. Which of the following best describes Scanning Electron Microscopy? a) Uses the properties of visible light to create flat images b) Uses a stream of electrons to create 3D images of the surface of the sample c) Uses chemically fluorescent dyes to create flat images of high contrast d) Uses a stream of electrons to create flat images of a thin section of a sample 12. The kind of ATP synthesis that occurs in the Electron Transport Chain in Cellular Respiration is called: a) substrate-level phosphorylation b) fermentation c) photophosphorylation d) oxidative phosphorylation Questions and answers for the mock midterm will be posted after Wednesday Dec. 3, 2014 at: http://www.usask.ca/ulc/sss, as well as to your blackboard course. 13. Which type of crossing experiment can be used to determine whether an individual is homozygous or heterozygous for a dominant allele? a) heterozygote crossed with a heterozygote b) F1 heterozygote crossed with a homozygous recessive individual c) Homozygous dominant crossed with a homozygous recessive d) Any of the above could be used 14. The result of epistasis is: a) Individuals with a variation of a disease from one extreme to another. b) When genes interact and both mask the effect of one another. c) Some expected phenotypes do not appear among offspring. d) The colour yellow in labradors is epistatic to the colour black. 15. In Drosophila, two genes, one for body colour and one for eye colour, are carried on the same chromosome. The wild type grey body colour is dominant to black body colour and wild-type red eyes are dominant to purple eyes. You make a cross between a fly with a grey body and red eyes and a fly with a black body and purple eyes. Among the offspring, about half have grey bodies and red eyes and half have black bodies and purple eyes. A small percentage have (a) black bodies and red eyes or (b) grey bodies and purple eyes. Which alleles are carried together on the chromosomes in each of the flies used in the cross? a) b+p+b+p+ x bpbp b) b+p+bp x bpbp c) b+p+b+p x bpbp d) b+p+bp+ x bpbp 16. Why are enzymes needed in biological systems? a) They are food for the cell b) They slow down reactions that occur too quickly c) They speed up reactions that occur too slowly d) They make the cell look cool when it’s with its other cell buddies 17. Which of the following sequences represent a correct order of enzymes required for DNA replication? a) Helicase, DNA polymerase, Primase, Ligase. b) Helicase, Ligase, DNA Polymerase, Primase. c) DNA Polymerase, Helicase, Primase, Ligase. d) Helicase, Primase, DNA Polymerase, Ligase. Questions and answers for the mock midterm will be posted after Wednesday Dec. 3, 2014 at: http://www.usask.ca/ulc/sss, as well as to your blackboard course. 18. Does all mRNA contain a start codon? a) No, because rRNA and tRNA do not code for proteins and therefore do not require start codons b) Yes, because mRNAs code for proteins and therefore require a start codon c) Yes, because rRNA and tRNA code for proteins and therefore require a start codon d) No, because mRNA does not code for protein and therefore does not need a start codon 19. What is the name of the sequence that directs growing amino acid chains on free ribosomes to the endomembrane system? a) Transit sequence b) Signal sequence c) Nuclear localization sequence d) Endomembrane sequence 20. The hydrolysis of ATP involves a) Adding water to ATP to release free energy b) Removing adenine from ATP to release free energy c) Removing water from ATP to release free energy d) Adding adenine to ATP to release free energy 21. Which one of the following occurs in the stroma of the chloroplast? a) Photophosphorylation b) Light reactions c) The Calvin cycle d) Electron transport chain 22. The Calvin cycle requires each of the following inputs EXCEPT: a) ATP b) O2 c) CO2 d) NADPH 23. NADH is the __________ form, while NAD+ is the __________ form. a) Reduced, oxidized. b) Neutral, positive. c) Oxidized, reduced. d) Organic, inorganic. Questions and answers for the mock midterm will be posted after Wednesday Dec. 3, 2014 at: http://www.usask.ca/ulc/sss, as well as to your blackboard course. 24. Rubisco, Ribulose-1,5-bisphosphate carboxylase/oxygenase, is the.. a) Sugar of the Calvin cycle that catalyzes the fixation of CO2 into organic form b) Sugar of photosynthesis that catalyzes the fixation of CO2 into inorganic form c) Enzyme of the Calvin cycle that catalyzes the fixation of CO2 into organic form d) Enzyme of the Calvin cycle that catalyzes the fixation of CO2 into inorganic form 25. Glycolysis is found in: a) Mammals b) All eukaryotes c) Bacteria and archaea d) All of the above 26. What occurs during glycolysis? a) Substrate level phosphorylation b) Reduction of glucose c) Oxidation of acetyl groups d) Oxidative phosphorylation 27. Any diploid individual may only have how many alleles of each individual gene? a) 23 b) 2 c) 46 d) 12 28. Which of the following is NOT a common cellular component of both eukaryotic and prokaryotic cells? a) Glycocalyx b) Plasma membrane c) Ribosomes d) Mitochondria 29. Glycolysis occurs in the __________, pyruvate oxidation occurs in the __________, and the citric acid cycle occurs in the __________________, respectively. a) Mitochondrion intermembrane compartment, mitochondrial outer membrane, cytosol. b) Cytosol, mitochondrial matrix, mitochondrial matrix. c) Mitochondrial matrix, mitochondrial matrix, cytosol. d) Cytosol, cytosol, mitochondrial matrix. Questions and answers for the mock midterm will be posted after Wednesday Dec. 3, 2014 at: http://www.usask.ca/ulc/sss, as well as to your blackboard course. 30. In glycolysis, _________ is invested, but will pay off by producing _________ and _________ (forms of energy), respectively. a) 2 ADP, 4 ATP, 2 NADH b) 2 ATP, 4 ATP, 2 NAD+ c) 2 ATP, 4 ATP, 2 NADH d) 2 ADP, 4 ATP, 2 NAD+ 31. What are the limitations of cell division? a) The cell needs to be at the correct stage of the cell cycle b) Time of the day c) Location in the multi-cellular organism d) The amount of mitotic spindles in the cell 32. The alleles for red eyes and blue hair segregate together as do the alleles for green eyes and brown hair, 86% of the time. Results from the same experiments find that the alleles for red eyes and brown hair segregate together as do the alleles for green eyes and blue hair, 14% of the time. Which alleles are linked together in the parents? a) Red eyes and brown hair in one parent b) Green eyes and blue hair in one parent c) green eyes and brown hair in one parent d) a) and b) 33. From the above question, how far apart are the genes on the chromosome? a) 43 map units b) 14 map units c) 86 map units d) 7 map units 34. Which of the differences between DNA and RNA are true? a) DNA uses cytosine, guanine, uracil, and adenine; whereas RNA uses cytosine, thymine, guanine, and adenine b) DNA has no free hydroxyl group on the 2’ carbon and RNA has a free hydroxyl group on the 2’ carbon c) DNA is usually single-stranded and RNA is usually double-stranded d) None of the above are true Questions and answers for the mock midterm will be posted after Wednesday Dec. 3, 2014 at: http://www.usask.ca/ulc/sss, as well as to your blackboard course. 35. A chromosome is undergoing DNA replication. If the template strand has a sequence of CGATTATCG, the enzyme primase will create a primer with which sequence (all sequences are written 5’ to 3’)? a) CGATTATCG b) CGAUUAUCG c) GCTAATAGC d) GCUAAUAGC 36. What is the product of one turn in the Calvin cycle? a) One molecule of CO2 is converted into one reduced carbon b) A three carbon molecule (G3P) c) ATP d) Glucose 37. How is ATP synthesized in photosynthesis? a) Rubisco b) A proton-motive force across the thylakoid membrane is used to synthesize ATP by chemiosmosis using the chloroplast ATP synthase c) A proton motive force is produced by NADH reduction and used by the ATP synthase d) Oxidative phosphorylation 38. During the analysis of a large pedigree, you find that there is little to no difference between the number of males and females that have a disease. What can you conclude about the disease? a) It is caused by an allele found on an autosomal chromosome b) It is caused by an allele found on either an autosomal or sex chromosome c) It is caused by an allele found on a sex chromosome d) It is caused by a dominant Y-chromosome located allele 39. A recently replicated DNA daughter helix has a 5’ to 3’ strand with many fragments ending in disconnected adjacent sugar and phosphate groups. This cell likely has a problem with which enzyme? a) DNA Polymerase b) Primase c) Ligase d) Helicase Questions and answers for the mock midterm will be posted after Wednesday Dec. 3, 2014 at: http://www.usask.ca/ulc/sss, as well as to your blackboard course. 40. Which series describes the structure of nucleic acids and their packaging in the cell from simplest unit to most complex? a) Nucleosome, Nucleotide, DNA Helix, Chromosome b) Nucleotide, Nucleosome, DNA Helix, Chromosome c) Nucleotide, DNA Helix, Nucleosome, Chromosome d) Nucleotide, DNA Helix, Chromosome, Nucleosome 41. The Smooth Endoplasmic Reticulum ____________: a) is where the cell synthesizes proteins. b) stores genetic material in the form of RNA. c) is the site of lipid synthesis in the cell. d) contains hydrolytic enzymes which digests old cellular parts. 42. What modifications must occur during mRNA processing to achieve a mature mRNA that is ready for translation? a) Addition of a polyadenosine tail b) Addition of 5’ methyl guanine cap c) Splicing out of introns and ligation of exons d) All of the above 43. Immediately following the Citric Acid Cycle in cellular respiration, all of the carbon atoms present in the original glucose molecule have: a) been used to form a lipid molecule b) become part of a new ATP molecule c) been released as carbon dioxide d) been freed into the cytosol as carbon ions 44. What are the three phases, in order, of cellular respiration? a) Glycolysis, oxidative phosphorylation, pyruvate oxidation/citric acid cycle. b) Glycolysis, pyruvate oxidation/citric acid cycle, oxidative fermentation c) Glycolysis, pyruvate oxidation/citric acid cycle, oxidative phosphorylation d) Glycolysis, phosphate oxidation/citric acid cycle, oxidation phosphorylation 45. Why is sugar oxidized in many small steps rather than burned all at once? a) To release more heat energy b) To transfer energy to other molecules c) To make the reaction become exergonic d) To increase the change in free energy Questions and answers for the mock midterm will be posted after Wednesday Dec. 3, 2014 at: http://www.usask.ca/ulc/sss, as well as to your blackboard course. 46. How would a cell transport a protein through its membrane? a) Endocytosis or exocytoisis b) Facilitated diffusion c) Active transport d) It depends on the polarity, charge, and size of the protein 47. Where are electrons donated to from photosystem I? a) A complex located on the lumen face of the thylakoid membrane called NADP+ reductase where NADP+ is reduced to NADPH b) A complex located on the lumen face of the thylakoid membrane called NADP+ reductase where NADPH is reduced to NADP+ c) A complex located on the stroma face of the thylakoid membrane called NADP+ reductase where NADP+ is reduced to NADPH d) A complex located on the stroma face of the thylakoid membrane called NADP+ reductase where NADPH is reduced to NADP+ 48. The electromagnetic spectrum contains a wide range of ER. However, in that range most of the radiation reaching the surface of the earth is contained in ultraviolet light, visible light and infrared radiation. Of visible light which of the following is true? a) Red light is more energetic and of a longer wavelength than blue light. b) Red light is less energetic and of a shorter wavelength than blue light. c) Red light is more energetic and of a shorter wavelength than blue light d) Red light is less energetic and of a longer wavelength than blue light 49. During the first phase of the Calvin Cycle, carbon dioxide is incorporated into ribulose bisphosphate by a) oxaloacetate b) G3P c) cytochrome oxidase d) rubisco 50. During redox reactions, the molecule that donates an electron is said to be a) Reduced b) Phosphorylated c) Oxidized d) Catabolized End of mock final Questions and answers for the mock midterm will be posted after Wednesday Dec. 3, 2014 at: http://www.usask.ca/ulc/sss, as well as to your blackboard course.