Download DNA Cot- I, human A7639 Comment

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DNA Cot- I, human
Synonym
Cot-1 DNA, human HS-No.:
38220000 Storage:
-20°C Shipment:
wet ice A7639
• human genomic DNA, predominantly consisting of rapdily
annealing repetitive elements • used to suppress crosshybridization to human repetitive
elements in hybridization experiments • supplied in 10 mM Tris (pH 7.5), 1 mM EDTA Specification
Concentration
1 mg/ml A260/A280
approx. 1.78 Average fragment size
50 - 300 bp non-repetitive genomic DNA
approx. 2 % Comment
Human DNA Cot-I is prepared exclusively from human placental DNA (male fetus only) by shearing, denaturing,
and reannealing under conditions that enrich repetitive elements. Therefore Cot-I fraction of human genomic DNA
predominatly consists of rapidly annealing repetitive elements. COT I Human DNA can be used for suppressing
crosshybridization to human repetitive DNA in filter and microarray hybridizations and in in situ hybridization
experiments. Repetitive elements present in a probe may result in unspecific hybridization signals. To enable
specific hybridization of the probe to the chromosomal target site the probe is denatured together with an excess
of unlabeled Cot-I human DNA as a competitor. As a result single-copy gene hybridization is not hindered by
repetitive sequences. The sample as well as the original material were tested for the absence of HIV1- and 2-RNA,
HCV-RNA, HBV-DNA.