Download GENETICS I. Review of DNA/RNA – A. Basic Structure – DNA 3

GENETICS
I.
Review of DNA/RNA –
A. Basic Structure – DNA
3 parts that make up a nucleotide
1. _______________________
2. _______________________
3. _______________________
___ chains wrap around each other to form a ___________
Chains run in opposite direction known as
______________________________
Type of bond between the sugar and the phosphate group
is known as __________________________
Type of bond between bases _________________
B. Basic Structure – RNA
Thymine is replaced by _________________________
# of strands ________
Name of pentose (5C sugar) ___________________
Leaves nucleus to travel to the ________________________
Drawing/Fxn
3 types: mRNA or ______________________
tRNA or _______________________
rRNA or _______________________
C. DNA Replication
List the main steps involved in DNA replication
List the enzymes involved in DNA replication and identify their function
D. Protein Synthesis
Transcription
DNA
Translation
mRNA
(Nucleus)
Protein (polypeptide)
(Cytoplasm/ribosome)
II.
TYPES OF INHERITANCE
A. Simple Mendelian
1. Monohybrid Cross
2. Dihybrid Cross
Probability:
Odds of a black card =
_____ (A)
Odds of an ace
_____ (B)
=
Odds of a black ace A x B = _____
Define:
3. Law of Segregation –
4. Law of Independent Assortment –
5. Exception to above are linked genes (define as well) –
Define and give example of:
B. Incomplete Dominance –
C. Co-Dominance –
D. Sex-Linked Trait (Typically, but not always, X-Linked) –
E. Some Common Genetic Disorders:
1. Autosomal Recessive
2. Autosomal Dominant
3. Sex-Linked
4. Chromosomal
III. Types of Mutations
Point mutation –
Frameshift mutation –
Chromosomal mutation – How is it tested for?
Polyploidy –
Mutagens –
Teratogens –
Carcinogens –
Karyotype
Pedigree
Modern Genetics
Recombinant DNA –
Plasmid –
Restriction Endonucleases –
Blunt Cut (HaeIII)
GGCC
CCGG
Restriction site between G & C 
GG
CC
Sticky Cut (HindIII)
AAGCTT
TTCGAA
Restriction site between A & A 
A AGCT
TTCGA A
Transformation –
Vector (Importance of phages) –
Cloning –
Introns (Intragenic regions) –
Exons (Expressed regions) –
DNA Fingerprinting (Gel Electrophoresis) –
CC
GG
1. During which meiotic phase are sister chromatids separated?
a) Prophase I
b) Metaphase I
c) Metaphase II
d) Anaphase I
e) Anaphase II
2. One cell that undergoes meiosis will yield how many independent cells?
a) 1
b) 2
c) 3
d) 4
e) 5
3. In which generation of a monohybrid cross do all the individuals look the same?
a) Parental (P)
b) F1
c) F2
d) F3
4. In a monohybrid cross, the two parental genotypes are AA and aa. The genotype of all F1 plants is Aa. If
the F1 plants are crossed to get an F2 generation, what percentage of the F2 generation will have the
dominant phenotype?
a) 0%
b) 25%
c) 50%
d) 75%
e) 100%
5. You are performing a dihybrid cross with two traits encoded by two different genes. These genes are on
two different chromosomes and follow the Law of Independent Assortment. The two parental genotypes
are AABB and aabb. The genotypes of all F1 plants are AaBb. What phenotypic ration will you observe in
the F2 generation?
a) 8:4:2:1
b) 1:1:1:1
c) 4:2:2:1
d) 1:2:2:1
e) 9:3:3:1
6. Tall is dominant over short; brown is dominant over green. The scientist wants to do a test cross to
determine the genotype of a tall, brown F1 hybrid. What will be the height and color of the other plant he
uses in this test cross?
a) Tall and brown
b) Tall and green
c) Short and brown
d) Short and green
e) It doesn't matter, any of the above will work
7. You are studying the inheritance of stem height and stem color. For height, tall is dominant over short and
for color brown is dominant over green. You mate a purebred tall, brown plant to a purebred short, green
plant and all of the F1 offspring are tall and brown. You then mate two of these F1 hybrid offspring together
and count the following phenotypes in the F2 offspring:
61 tall and brown; 1 tall and green; 2 short and brown; 18 short and green.
Which of the following is probably true?
a) The two genes controlling these traits are sex-linked
b) The two genes controlling these traits might be on different chromosomes
c) The two genes controlling these traits are linked with no crossing over
d) The two genes controlling these traits are nearby on the same chromosome
e) The two genes controlling these traits are far apart on the same chromosome
8. Which of the following is usually NOT true of an autosomal recessive disease?
a) Most people with the disease have parent who do not have the disease
b) An equal number of males and females get the disease
c) If both parent have the disease, 50% of their offspring will have the disease
d) If both parents are heterozygous, 75% of their offspring will not get the disease
e) The genes for the disease are not located on the sex chromosomes
9. A woman who is heterozygous for hemophilia marries a man who has hemophilia. What percentage of their
female offspring would you expect to have hemophilia?
a) 0%
d) 75%
b) 25%
e) 100%
c) 50%
10. A boy with red-green color-blindness has a color-blind father and a mother who is not color-blind. From
which parent did the boy inherit the color-blind allele?
a) No way to know – it could have been either one
b) His father
c) His mother
d) It had to come partly from his mother and father
e) It had nothing to do with either his mother or father
Refer to the pedigree below to answer Questions 11-14
11. Based on the chart, the allele causing the shaded trait is likely
a) Codominant
b) X-linked recessive
c) Autosomal dominant
d) Y-linked
e) X-linked dominant
12. What is the probability that individual 3 is a carrier?
a) 0%
b) 25%
c) 50%
d) 75%
e) 100%
13. What is the probability that individual 2 is a carrier?
a) 0%
b) 25%
c) 50%
d) 75%
e) 100%
14. In order for #4 to have a son expressing the phenotype, he could mate with
I. A female carrier
II. An affected female
III. A non-carrier female
a) I only
b) II only
c) III only
d) I and II only
e) I, II and III
Refer to the following diagram to answer Questions 15-18
15. This transfer of genetic material is called
a) Transcription
b) Transduction
c) Crossing-over
d) Translation
e) substitution
16. The depicted exchange of genetic material can occur during
a) Prophase I of meiosis
b) Anaphase I of meiosis
c) Telophase of mitosis
d) Metaphase II of meiosis
e) Interphase of mitosis
17. After the chromosomes exchange material, which of the following could NOT result in the next generation?
a) Adaptive mutation
b) Same phenotype
c) Debilitating mutation
d) Change in diploid number
e) Further transfers of genetic material
18. The exchange of genetic material only occurs in sexual reproduction, and offers a significant advantage
over asexual reproduction. Why?
a) Genetic recombination increases variation of species
b) Random mutations are usually beneficial
c) It alters the number of base pairs in the species' genetic code
d) It increases reproduction rates
e) It provides stability in the gene pool
1)c
10)c
2)d
11) b
3)b
12)c
4)d
13)c
5)e
14)d
6)d
15)c
7)c
16)a
8)c
17)b
9)c
18)a