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The plant cell. Antonie van Leeuwenhoek & Robert Hooke 1 centimeter (cm) =1/10 meter = 0.4 inch 1 millimeter (mm) = 1/1000 meter = 1/ 10 cm 1 micrometer (µm) = 1/1 ,000, 000 meter 1/ 10, 000 cm 1 nanometer (nm) = 1/1000, 000, 000 meter = 1/ 10, 000, 000 1 Angstrom (Å) = 1/ 10,000,000,000 meter = 1/ 100, 000, 000 = 1/10 nm 1 meter = 102 cm = 103 mm = 106 µm = 109 nm = 1010 Å Stereoscopic or "dissec-ng microscope” Compound light microscope Light thousand times -----0.2 µm Transmission electron microscope electrons Hundred of thousands times ----0.0002 µm Scanning electron microscope electrons One million times Metal < 0.0002 µm Cell theory 1) Living organisms are composed for one or more cells 2) Energy-‐ releasing and biosynthe?c reac?ons take place within the cells 3) Cells arise from other cells 4) Hereditary informa?on pass from one genera?on to the other vacuoles Vacuoles have a single membrane: The tonoplast the vacuole is the largest compartment of most mature plant cells. Main functions of the vacuole • Isolating harmful materials • Containing waste products • Maintaining turgor within the cell • Maintaining an acidic internal pH • Containing pigments and sap Calcium oxaloacetate crystals a) Druses b) Raphides Plamolysis components of the cell wall The cell wall is a cross-linked matrix of: Cellulose Lignin Hemicelluloses: regulate the cell enlargement Pectin: attract water to the walls and form the middle lamella Glycoproteins: structural proteins The arrows indicates the middle lamella Primary cell walls Unevenly thickened walls. Primary pit fields and plasmodesmata Primary cell walls Undifferen?ated cells Support Secondary wall Alkaloids Collenchyma cellulose and pectin structural support for shoots and leaves Sclerenchyma Tissues that have ceased elongation. Mature sclerenchyma is composed of dead cells Cellulose, hemicellulose, and lignin. Fibers (jute, hemp, henequen) Secondary wall Sclereids: small bundles of sclerenchyma tissue in plants that form durable layers, such as the cores of apples and the gritty texture of pears. astrosclereids Cell wall In a secondary wall commonly we can find S1 Outer layer S2 Middle layer S3 Inner layer In order to increase the thickness and the surface area new cellulose micro fibrils are placed on top of those previously formed Cellulose synthase expansins Attendance 1. Define tonoplast 2. What is plasmolysis? 3. Why are pec?nes important? 4. What is the difference between primary pit fields and plasmodesmata? 5. What are sclereids? 6. Draw the structure of the complete cell wall and explain how a primary cell wall differs from a secondary cell wall