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Transcript
Chapter 16 Outline Gene Technology Advanced Placement Biology Roslyn High School Molecular Biologists Can Manipulate DNA To Clone Genes. What’s In The DNA Manipulator’s Toolbox? How Are Restriction Endonucleases Used? How Is Ligase Used? What Are The Host/Vector Systems? Why Are Plasmids Used? What Is The Function Of Phages? How Are Vectors Used To Transfer Genes? How Was Psc101 Constructed? How Was Psc101 Used To Make Recombinant DNA? What Are Some Other Vectors? How Are DNA Libraries Created? What Are Genomic Libraries? What Are cDNA Libraries? Genetic Engineering Involves Easily Understood Procedures. How Are The Four Stages Of Genetic Engineering Experiments Performed? Stage 1 - DNA Cleavage Stage 2 – Production Of Recombinant DNA Stage 3 – Cloning Stage 4 – Screening (Including Screening Of Clones And For The Gene Of Interest) How Can We Work With Gene Clones? How Can You Get Enough DNA To Work With By Using The Polymerase Chain Reaction? How Can You Identify DNA Using Southern Blotting? How Can You Distinguish Differences In DNA Using RFLP Analysis? How Is DNA Fingerprinting Performed? Biotechnology Is Producing A Scientific Revolution. What Are The Medical Applications Of: Pharmaceuticals? Gene Therapy? Piggyback Vaccines? What Are The Agricultural Applications? Nitrogen Fixation? Herbicide Resistance? Insect Resistance? What Is The Real Promise Of Plant Genetic Engineering? Farm Animals? What Are The Risks And Regulations? How Do We Measure The Potential Risks Of Genetically Modified Crops? Is Eating Genetically Modified Food Dangerous? Are Genetically Modified Crops Harmful To The Environment? Should We Label Genetically Modified Foods?
