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La Serna High School Biology Final Exam Review Guide – Semester I 2012-2013 ANSWER KEY Investigation & Experimentation (I&E 1c,e,f) 1. Define Hypothesis: An educated guess before doing an experiment 2. Define Theory: A well tested explanation that unifies a broad range of observations 3. What is a controlled experiment? An experiment that only has ONE independent variable, all other variables are controlled 4. Why is a control group important to have in an experiment? It is used a basis for comparison, you have reliable results 5. Define Independent Variable: The variable in the experiment that is purposely changed by the experimenter 6. Define Dependent Variable: The variable that the experimenter is trying to measure or observe 7. Explain possible sources of errors in an experiment: There can be avoidable or unavoidable errors, but errors occur when there is more than one independent variable. Review and practice reading graphs and interpreting results. Macromolecules (Cell Biology 1b,h) 8. Define monomer: a small single unit that forms polymers when combined 9. Define polymer: a large molecule made from many small individual subunits 10. Fill in the chart below. Monomer Simple Sugars (nonsaccharides) Amino Acids Fatty acids Nucleotides Polymer Carbohydrates (Polysaccharides) Proteins Lipids Nucleic Acids Common Example Sugars, starches, glucose, cellulose Hair, Skin, nails, enzymes Waxes, oils, fats, steroids DNA and RNA 11. What does Benedict’s solution test for? Explain a positive test. Benedict’s solution tests for the presence of monosaccharides or simple sugars. In a positive test the liquid goes from a blue color to an orange/red after it is heated. 12. Define denature: It means that the shape of a protein has been permanently changed 13. Define catalyst: It speeds up a reaction by lowering activation energy 14. What is an enzyme? An enzyme is a type of protein that works like a catalyst. It speeds up reaction by lowering activation energy. 15. What factors can reduce the activity of an enzyme? Temperature, pH level, Ionic conditions or overuse. All of these would make the enzyme not work as efficiently. 16. Describe the process in the figure below. a. Enzyme attaches to specific substrate b. Enzyme and substrate interact at Active Site, lowering activation energy of the reaction. c. Reaction is complete when products are formed. d. Enzyme is free to start the process again. (recycled in the body) e. Your body can turn enzymes “on” and “off” at specific times. (when specific products are needed) 17. Does the enzyme change during this process? No, the enzyme may be recycled or used again. 18. What happens to the enzyme following this process? The enzyme is free to begin the next reaction Cell Structure & Function (Cell Biology 1a-e) 19. List the three part of the Cell Theory. 20. What is the difference between prokaryotic and eukaryotic cells? Eukaryotic Cells have a nucleus and many organelles, prokaryotic cells do not. 21. Give an example of a prokaryotic cell: Bacteria 22. Give at least two examples of eukaryotic cells: Plant cells and animal cells 23. What is a virus? DNA and a protein coat (NOT a cell) 24. Why are viruses NOT considered living cells? They can not reproduce on their own 25. What is an organelle? A tiny structure within a eukaryotic cell that serves a specific function 26. Label the cell diagram to the right. 1) Endoplasmic reticulum (Smooth or Rough ER) 3 2) Nucleus 2 3) Mitochondria 4) Golgi apparatus 4 5) Cell membrane 5 27. What is the function of a ribosome? To assemble proteins 28. What is the function of the endoplasmic reticulum? To modify (or reshape) the amino acid chain/protein and package the protein 29. What is the function of the Golgi apparatus? To send the protein to its destination either inside the cell or exported outside the cell through exocytosis 30. What are the functions of the cell membrane? To control what enters and exits the cell, the cell membrane maintains homeostasis 31. Define semi-permeable: It allows some molecules to pass in and out and keeps others out 32. Define Passive Transport: Molecules moving from an area of high concentration to low concentration, does not require energy 33. What occurs during Osmosis: the diffusion of water molecules across a membrane 34. Define Active Transport: Molecules moving from an area of low concentration to high concentration, it DOES require energy Cellular Energy (Cell Biology 1f,g) 35. What is the overall chemical reaction of photosynthesis? CO2 + H2O + light energy O2 + C6H12O6 (O2 is released as a waste product) 36. Rewrite the equation above using words (instead of chemical formulas). Draw a circle around the PRODUCTS! Carbon Dioxide and water and energy from the sun will produce oxygen, and glucose (which the plant uses as food) 37. What role does chlorophyll have in photosynthesis? Chlorophyll is the green pigment in the chloroplast that absorbs sunlight energy 38. In which macromolecule group does glucose belong? Glucose is a simple sugar in the carbohydrates group 39. What is the overall chemical reaction of cellular respiration? O2 + C6H12O6 CO2 + H2O + Energy (ATP) 40. Rewrite the equation above using words (instead of chemical formulas). Draw a circle around the PRODUCTS! Oxygen (from the atmosphere) and glucose (the breakdown of food) will produce carbon dioxide (given off as waste), water and ATP energy 41. In which organelle does cellular respiration occur? In the mitochondria 42. Draw a simple diagram of ATP and explain how it releases energy. ATP releases energy when one of the three phosphate groups is removed. 43. What are the three main stages of cellular respiration (in order)? 1) Glycolysis- takes place in the cytoplasm and produces a total of 4 ATP molecules, but requires 2 ATP to work 2) Kreb’s cycle (AKA citric acid cycle)- takes place in the matrix of the mitochondria and produces 2 ATP molecules 3) Electron Transport Chain- takes place in the cristae (inner membrane) of the mitochondria and produces 32 ATP! 44. What is the main product of cellular respiration? ATP molecules 45. What was broken down during cellular respiration to produce the product above? Glucose, in the presence of oxygen DNA & Protein Synthesis (Cell Biology 1d, Genetics 4a-e & 5a-c) 46. Label the parts of the DNA molecule to the right. A. guanine B. cytosine C. thymine D. adenine E. sugar-phosphate backbone F.nucleotide 47. Do all cells in a single person contain the same DNA sequence? YES 48. If all cells of a multicellular organism contain the same genes, explain how they appear and function differently? The cells use different portions of DNA, different introns and exons. Different genes are expressed in different cells. 49. Describe the nitrogen base-pairing rules for DNA. A pairs with T and G pairs with C 50. The process of information encoded in DNA being rewritten as mRNA is called transcription. 51. Determine the mRNA sequence that would be produced from this original template DNA strand. DNA = 5` A T G G C T A 3` mRNA= U A C C G A U 52. What are the 3 main differences between DNA and RNA? RNA is: a. Ribose sugar instead of Deoxyribose b. Single stranded c. Uracil instead of thymine 53. The process of cells using information from mRNA to produce proteins is called protein synthesis 54. Label each of the parts in the diagram to the right. A. polypeptide chain or amino acid chain or protein B. peptide bonds C. tRNA molecule D. amino acid E. codon F. mRNA strand G. ribosome 55. What type of bonds connects amino acids to produce a protein? Peptide bonds 56. Which amino acids would the mRNA sequence AUGACC produce? Codons Found in Messenger RNA Met-Asn 57. List all mRNA codons that code for the amino acid alanine. GCU, GCC, GCA, GCG 58. What is the reason for so many different proteins existing, when there are only 20 amino acids? A different number or a different sequence of amino acids will produce a different protein. 59. Define mutation: Any change naturally occurring in a DNA sequence 60. What are 2 types of mutations? Frameshift, translocation, substitution, deletion 61. What are some possible causes of mutations? 62. What could happen as a result of a mutation? Most mutations have no effect, some mutations are harmful, but can also sometimes be beneficial.