Download doc BIOL200 quiz 4 afternoon

X = the answers
a-helix:
why statements is/are true?
Question options:
X
produces straight rigid rods
X
can be half buried in the core of the protein and half exposed to the
surrounding watery environment
has always a precise periodicity in the R side-chains, with charged
residues evenly distributed every 3 amino acids
has a periodicity of 2.5 amino acids
is never embedded in a lipid membrane
X
has all the R residues oriented outwards
is held together by hydrogen bonds between neighboring side-chains "R"
of the polar amino acids
Question 2
Can the following be found in a cDNA sequence?
Question options:
X
start codon
the promoter
X
3' untranslated region (UTR)
an intron
a splicing consensus sequence
Question 3
A fragment "F" of DNA must be inserted into the vector "V". The aim is
to construct a recombinant plasmid that will be used to express the
gene product codes by the insert sequence. Here below are the
restrictions maps of the vector and the insert.
Which of the following enzyme digestions could be used for this
construction?
Question options:
Vector: EcoR1 Apa1; fragment: EcoR1 SnaB1
Vector: EcoR1 Xba1; fragment: EcoR1 Spe1
X
Vector: SnaB1 Xba1; fragment: Stu1 Xba1
Vector: SnaB1 Spe1; fragment: Stu1 Xba1
Vector: SnaB1 Spe1; fragment: Stu1 Spe1
Vector: EcoR1 SnaB1, fragment: EcoR1 Stu1
X
Both vector and fragment: EcoR1 Apa1
Vector: Kpn1 Xba1; fragment: Asp718 and Xba1
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Which of these statements is/are true?
Question options:
X
Plasmids can be used to clone sequences of about the size of an average
gene coding sequence.
X
Bacteria can grow on solid agar or in liquid culture
X
Plasmids are naturally occurring in bacteria, and can transfer genetic
information to other bacteria.
The reason why plasmids engineered by molecular biologists are
abundant in transformed bacteria and can yield large amounts of DNA is
that they are equally segregated during bacterial division.
Foreign DNA can only be introduced in unicellular organisms.
Bacteria look different when they have been transformed with a DNA
plasmid and thus can be selected according to morphological criteria.
Question 5
Which of the following statements is/are correct?
Question options:
The only way to detect the presence of a plasmid in bacteria is to screen by
PCR.
X
P-elements are transposable elements in Drosophila that can disrupt genes,
producing a "natural" knock out
It is easier to express a recombinant protein in bacteria because the DNA
plasmid is more readily imported in the nucleus and incorporated in a
chromosome.
A "knock down" is a targeted mutation that leads to decreased transcription of
the targeted gene.
X
A gene introduced by "knock in" should be expressed similarly to the original
"endogenous" gene because it will be under the control of the same promoter
and of the same regulatory sequences.