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Transcript
Wet Mount Put a Tiny Amount of Discharge on a Microscope Slide. Make this as small as possible. Add one drop of Normal Saline (0.9% NaCl) to the drop of discharge. Mix well on the slide. Make a 2nd slide in the same way, using 10 percent KOH.. Place glass coverslips over the slides. Remove excess fluid with tissue paper. Wait 2 minutes for the cell membranes to dissolve, or heat the KOH slide to speed the dissolving process. Examine the prepared slides under a microscope. The lowest power (~ 40X) works the best. OB-GYN 101 Facts Card ©2003 Brookside Press cells can be seen. Sometimes, it has the appearance of a tangled web of threads. At other times, only small branches will be seen. Yeast normally live in the vagina, but only in very small numbers. If you visualize any yeast in your sample, it is considered significant. Trichomonas is best seen on the Normal Saline slide. These protozoans are about the same size as a white blood cell (a little smaller than a vaginal epithelial cell), but their violent motion is striking and unmistakable. Yeast (Candida, Monilia) is best identified with the KOH slide. Bacterial vaginosis (also known as Gardnerella, hemophilus, or nonspecific vaginitis) is characterized by the presence of "clue cells" visible at both low and medium power. After the cell membranes are dissolved, the typical branching and budding yeast These clue cells are vaginal epithelial cells studded with bacteria. It resembles a pancake that has fallen into a bowl of poppy seeds, but on a microscopic level. A normal vaginal epithelial cell is clear, with recognizable contents, and sharp, distinct cell borders. A clue cell appears smudged, with indistinct contents and fuzzy, poorly defined borders.