REPLICATION, TRANSCRIPTION, TRANSLATION TAKS
... 14 Part of a DNA strand is represented in the diagram above. In order for DNA to replicate, the strand must separate at which of the following locations? F Between every phosphate-sugar pair G Between the eight sugar-base pairs H* Between the four nitrogenous base pairs J Between any two chemical bo ...
... 14 Part of a DNA strand is represented in the diagram above. In order for DNA to replicate, the strand must separate at which of the following locations? F Between every phosphate-sugar pair G Between the eight sugar-base pairs H* Between the four nitrogenous base pairs J Between any two chemical bo ...
22. Recombinant DNA Technology
... Restriction endonuclease and DNA ligase yield Recombinant DNA ...
... Restriction endonuclease and DNA ligase yield Recombinant DNA ...
2015 Chaffey College Poster
... among fish species, and part of the gene being highly variable causing the fish to express different traits and look different. The sequence targeted in this case is the common gene on the DNA of ...
... among fish species, and part of the gene being highly variable causing the fish to express different traits and look different. The sequence targeted in this case is the common gene on the DNA of ...
Restriction Enzymes - Seattle Central College
... • Bacteriophage attacks bacteria by inserting its nucleic acid into the host bacteria which replicates rapidly inside host cells until cells burst. The released phage carries out the infection in other bacteria. 3. Agarose Gel Electrophoresis • The fragments of DNA can be visualized through gel elec ...
... • Bacteriophage attacks bacteria by inserting its nucleic acid into the host bacteria which replicates rapidly inside host cells until cells burst. The released phage carries out the infection in other bacteria. 3. Agarose Gel Electrophoresis • The fragments of DNA can be visualized through gel elec ...
ELECTROPHORESIS
... 1- The identification of certain molecules. 2- The isolation of a certain molecule. 3- The molecular weight of certain molecules. In this lecture, Polyacrylamide Gel Electrophoresis (PAGE) and "Hemoglobin Electrophoresis" are introduced. ...
... 1- The identification of certain molecules. 2- The isolation of a certain molecule. 3- The molecular weight of certain molecules. In this lecture, Polyacrylamide Gel Electrophoresis (PAGE) and "Hemoglobin Electrophoresis" are introduced. ...
4.13 notes
... • there are two types of nucleic acid: DNA and RNA Nucleotides • a nucleotide is made of three parts (see figure to right): • a phosphate group • a 5-carbon sugar (DNA has deoxyribose, RNA has ribose) • a nitrogen base (there are five different bases available) RNA • is made of a single-stranded cha ...
... • there are two types of nucleic acid: DNA and RNA Nucleotides • a nucleotide is made of three parts (see figure to right): • a phosphate group • a 5-carbon sugar (DNA has deoxyribose, RNA has ribose) • a nitrogen base (there are five different bases available) RNA • is made of a single-stranded cha ...
Biology Packet 7: DNA & RNA
... Explain the function of DNA. Summarize the relationship between genes and DNA. Describe the overall structure of the DNA molecule. Describe the three components of a nucleotide. Explain the base pairing rules. Relate the role of the base pairing rules to the structure of DNA. Summarize the events of ...
... Explain the function of DNA. Summarize the relationship between genes and DNA. Describe the overall structure of the DNA molecule. Describe the three components of a nucleotide. Explain the base pairing rules. Relate the role of the base pairing rules to the structure of DNA. Summarize the events of ...
Biotech unit Objectives
... Wells Agarose gel recombinant DNA stem cells RFLP analysis sticky ends restriction endonucleases hybridization plasmid mapping primer tracking dye lane marker genetically modified foods electroporation ...
... Wells Agarose gel recombinant DNA stem cells RFLP analysis sticky ends restriction endonucleases hybridization plasmid mapping primer tracking dye lane marker genetically modified foods electroporation ...
Biotechnology Unit Test Review
... (1-7) Define the following terms: 1. Recombinant DNA – sequence of DNA made from two or more species (in class, we combined a human gene with a bacterial DNA plasmid) 2. Restriction enzymes – Bacterial enzymes that cut DNA in specific places (look for specific nitrogen base sequences and make zig-za ...
... (1-7) Define the following terms: 1. Recombinant DNA – sequence of DNA made from two or more species (in class, we combined a human gene with a bacterial DNA plasmid) 2. Restriction enzymes – Bacterial enzymes that cut DNA in specific places (look for specific nitrogen base sequences and make zig-za ...
C13 Genetic Engineering
... DNA electrophoresis, the DNA cut with restriction enzymes is put into the well at one end (negative end – black) of the gel. DNA molecules are negatively charged and will travel to the positive end when current is applied. The smaller fragments travel faster. We can use this to get the DNA fingerpri ...
... DNA electrophoresis, the DNA cut with restriction enzymes is put into the well at one end (negative end – black) of the gel. DNA molecules are negatively charged and will travel to the positive end when current is applied. The smaller fragments travel faster. We can use this to get the DNA fingerpri ...
Prof. Mario Feingold – Dept. of Physics
... Single Molecule Studies of DNA-protein interactions - We use Optical Tweezers to manipulated single DNA molecules. This method can be used to probe various processes in which the DNA plays a role. In particular, we propose to use this approach to study the interaction between the DNA and sequence sp ...
... Single Molecule Studies of DNA-protein interactions - We use Optical Tweezers to manipulated single DNA molecules. This method can be used to probe various processes in which the DNA plays a role. In particular, we propose to use this approach to study the interaction between the DNA and sequence sp ...
R 9.1
... biotechnology. Some examples include sequencing genes, copying (or cloning) genes, chemically mutating genes, analyzing and organizing genetic information with computer databases, and transferring genes between organisms. In many of these research areas, DNA must first be cut so that it can be studi ...
... biotechnology. Some examples include sequencing genes, copying (or cloning) genes, chemically mutating genes, analyzing and organizing genetic information with computer databases, and transferring genes between organisms. In many of these research areas, DNA must first be cut so that it can be studi ...
AP-ppt-PCR
... RFLP’s-Restriction Fragment Length Polymorphisms Variations in the length of fragments resulting from action by a specific restriction enzyme uses ...
... RFLP’s-Restriction Fragment Length Polymorphisms Variations in the length of fragments resulting from action by a specific restriction enzyme uses ...
Chapter 11 Concept Check Questions
... 3. Desribe the experimental design that allowed Hershey and Chase to distinguish between the two options for genetic material. ...
... 3. Desribe the experimental design that allowed Hershey and Chase to distinguish between the two options for genetic material. ...
Microbiology Unit 3 Study Guide
... 4. How would you transcribe the following DNA sequence? ATA CGT CAT AAG 5. Which enzyme makes RNA by reading a strand of DNA? 6. Which enzymes cut DNA in specific locations? 7. What occurs during transcription? 8. What are the steps to obtaining DNA fragments for gel electrophoresis? 9. Which enzyme ...
... 4. How would you transcribe the following DNA sequence? ATA CGT CAT AAG 5. Which enzyme makes RNA by reading a strand of DNA? 6. Which enzymes cut DNA in specific locations? 7. What occurs during transcription? 8. What are the steps to obtaining DNA fragments for gel electrophoresis? 9. Which enzyme ...
DNA Fingerprinting at Imperial College London 2015 PDF File
... DNA Fingerprinting at Imperial College London Ever wondered how DNA is used to identify people in forensic science or for paternity tests? Ten Y12 students were lucky enough to have an opportunity to discover just that at the impressive laboratories of Imperial College London. The students were give ...
... DNA Fingerprinting at Imperial College London Ever wondered how DNA is used to identify people in forensic science or for paternity tests? Ten Y12 students were lucky enough to have an opportunity to discover just that at the impressive laboratories of Imperial College London. The students were give ...
Gel electrophoresis of nucleic acids
Nucleic acid electrophoresis is an analytical technique used to separate DNA or RNA fragments by size and reactivity. Nucleic acid molecules which are to be analyzed are set upon a viscous medium, the gel, where an electric field induces the nucleic acids to migrate toward the anode, due to the net negative charge of the sugar-phosphate backbone of the nucleic acid chain. The separation of these fragments is accomplished by exploiting the mobilities with which different sized molecules are able to pass through the gel. Longer molecules migrate more slowly because they experience more resistance within the gel. Because the size of the molecule affects its mobility, smaller fragments end up nearer to the anode than longer ones in a given period. After some time, the voltage is removed and the fragmentation gradient is analyzed. For larger separations between similar sized fragments, either the voltage or run time can be increased. Extended runs across a low voltage gel yield the most accurate resolution. Voltage is, however, not the sole factor in determining electrophoresis of nucleic acids.The nucleic acid to be separated can be prepared in several ways before separation by electrophoresis. In the case of large DNA molecules, the DNA is frequently cut into smaller fragments using a DNA restriction endonuclease (or restriction enzyme). In other instances, such as PCR amplified samples, enzymes present in the sample that might affect the separation of the molecules are removed through various means before analysis. Once the nucleic acid is properly prepared, the samples of the nucleic acid solution are placed in the wells of the gel and a voltage is applied across the gel for a specified amount of time.The DNA fragments of different lengths are visualized using a fluorescent dye specific for DNA, such as ethidium bromide. The gel shows bands corresponding to different nucleic acid molecules populations with different molecular weight. Fragment size is usually reported in ""nucleotides"", ""base pairs"" or ""kb"" (for thousands of base pairs) depending upon whether single- or double-stranded nucleic acid has been separated. Fragment size determination is typically done by comparison to commercially available DNA markers containing linear DNA fragments of known length.The types of gel most commonly used for nucleic acid electrophoresis are agarose (for relatively long DNA molecules) and polyacrylamide (for high resolution of short DNA molecules, for example in DNA sequencing). Gels have conventionally been run in a ""slab"" format such as that shown in the figure, but capillary electrophoresis has become important for applications such as high-throughput DNA sequencing. Electrophoresis techniques used in the assessment of DNA damage include alkaline gel electrophoresis and pulsed field gel electrophoresis.For short DNA segments such as 20 to 60 bp double stranded DNA, running them in Polyacrylamide gel (PAGE) will give better resolution(native condition). Similarly, RNA and single stranded DNA can be run and visualised by PAGE gels containing denaturing agents such as Urea. PAGE gels are widely used in techniques such as DNA foot printing, EMSA and other DNA-protein interaction techniques.The measurement and analysis are mostly done with a specialized gel analysis software. Capillary electrophoresis results are typically displayed in a trace view called an electropherogram.