Use the diagram to match the letter (A-C) to the correct term(1
... 6. ______ Individual nitrogen base. 7. ______ Sugar-phosphate backbone. 8. In DNA, which of the following determines the traits of an organism? a. Amount of adenine b. Number of sugars c. Sequence of nitrogen bases d. Strength of hydrogen bonds 9. You have separated the nucleotides in a piece of DNA ...
... 6. ______ Individual nitrogen base. 7. ______ Sugar-phosphate backbone. 8. In DNA, which of the following determines the traits of an organism? a. Amount of adenine b. Number of sugars c. Sequence of nitrogen bases d. Strength of hydrogen bonds 9. You have separated the nucleotides in a piece of DNA ...
DNA to Protein - Duplin County Schools
... http://www.classzone.com/cz/books/bio_07/resources/htmls/interactive_review/bio_intrev.html ...
... http://www.classzone.com/cz/books/bio_07/resources/htmls/interactive_review/bio_intrev.html ...
SafeView - NBS Biologicals
... This dye replaces Ethidium Bromide (toxic, potential mutagen) for visualisation of DNA or RNA in Agarose gel. SafeView is noncarcinogenic and causes significantly fewer mutations in the Ames-test and tests negative in both the mouse marrow chromophilous erythrocyte micronucleus test and mouse sperma ...
... This dye replaces Ethidium Bromide (toxic, potential mutagen) for visualisation of DNA or RNA in Agarose gel. SafeView is noncarcinogenic and causes significantly fewer mutations in the Ames-test and tests negative in both the mouse marrow chromophilous erythrocyte micronucleus test and mouse sperma ...
Discovery of DNA
... Discovery of DNA Alfred Hershey & Martha Chase • Question: Are genes made of DNA or proteins? • What they knew: viruses use other organisms to reproduce Viruses only contain DNA and a protein coat. Whichever virus particle enters the cell must be the material that makes up genes (DNA). ...
... Discovery of DNA Alfred Hershey & Martha Chase • Question: Are genes made of DNA or proteins? • What they knew: viruses use other organisms to reproduce Viruses only contain DNA and a protein coat. Whichever virus particle enters the cell must be the material that makes up genes (DNA). ...
Biotechnology
... • Restriction fragment length polymorphisms (RFLPs) are SNPs that change the length of restriction fragments ...
... • Restriction fragment length polymorphisms (RFLPs) are SNPs that change the length of restriction fragments ...
Ethidium Bromide
... The Establishment of Purity and the Separation of DNA Strands by Electrophoresis "Electrophoresis of DNA in agarose minigels containing ethidium bromide provides a rapid method of measuring both the quantity of DNA and its purity. Minigels are poured on 5 cm x 8 cm glass plates and sample slots are ...
... The Establishment of Purity and the Separation of DNA Strands by Electrophoresis "Electrophoresis of DNA in agarose minigels containing ethidium bromide provides a rapid method of measuring both the quantity of DNA and its purity. Minigels are poured on 5 cm x 8 cm glass plates and sample slots are ...
DNA – The Double Helix
... within the cell; which proteins are made is determined by the sequence of the DNA. Proteins are the building blocks of an organism. How you look is largely determined by the proteins that are made. ...
... within the cell; which proteins are made is determined by the sequence of the DNA. Proteins are the building blocks of an organism. How you look is largely determined by the proteins that are made. ...
Genetic Engineering and The Human Genome
... **Important because it could be used to cure genetic disorders ...
... **Important because it could be used to cure genetic disorders ...
24 October - web.biosci.utexas.edu
... posted on the course website. PRINT it out and turn it in either on your discussion sections or on next Monday's class no later than 12:00PM. Email attachments and late delivery are not acceptable. 1. What factors ensure the fidelity of replication during DNA synthesis? 2. Define “promoter” and disc ...
... posted on the course website. PRINT it out and turn it in either on your discussion sections or on next Monday's class no later than 12:00PM. Email attachments and late delivery are not acceptable. 1. What factors ensure the fidelity of replication during DNA synthesis? 2. Define “promoter” and disc ...
Reproduction
... Deoxyribonucleic acid (DNA) and bonucIeic acid (ANA) are two of the cell’s most Important molecules. These nucleic acids have a complex three-dimensional structure that enab les them to direct protein synthesis in the cell. • Study the structure of the DNA and RNA molecules shown below. Fill in the ...
... Deoxyribonucleic acid (DNA) and bonucIeic acid (ANA) are two of the cell’s most Important molecules. These nucleic acids have a complex three-dimensional structure that enab les them to direct protein synthesis in the cell. • Study the structure of the DNA and RNA molecules shown below. Fill in the ...
Cytosine – ______ Sugar
... 2. Draw a guanine nucleotide based on Figure 12-5. Label each part of the nucleotide. ...
... 2. Draw a guanine nucleotide based on Figure 12-5. Label each part of the nucleotide. ...
After Gel Electrophoresis…
... A technique used in genetic engineering that separates DNA fragments by applying an electric charge to a porous gel ...
... A technique used in genetic engineering that separates DNA fragments by applying an electric charge to a porous gel ...
Introduction to gel electrophoresis
... 25Kb DNA fragments. • DNA has negatively charged phosphates along the DNA backbone. ...
... 25Kb DNA fragments. • DNA has negatively charged phosphates along the DNA backbone. ...
Laboratory Exam I - HCC Learning Web
... 26. What are Barr bodies? When are they present in the cell cycle. 27. What is a restriction enzyme? What do they do? 28. What is the charge of DNA when immersed in a basic medium? Why is the pH so important when performing gel electrophoresis? 29. A tube contains a mixture of DNA fragments of the f ...
... 26. What are Barr bodies? When are they present in the cell cycle. 27. What is a restriction enzyme? What do they do? 28. What is the charge of DNA when immersed in a basic medium? Why is the pH so important when performing gel electrophoresis? 29. A tube contains a mixture of DNA fragments of the f ...
Gel electrophoresis of nucleic acids
Nucleic acid electrophoresis is an analytical technique used to separate DNA or RNA fragments by size and reactivity. Nucleic acid molecules which are to be analyzed are set upon a viscous medium, the gel, where an electric field induces the nucleic acids to migrate toward the anode, due to the net negative charge of the sugar-phosphate backbone of the nucleic acid chain. The separation of these fragments is accomplished by exploiting the mobilities with which different sized molecules are able to pass through the gel. Longer molecules migrate more slowly because they experience more resistance within the gel. Because the size of the molecule affects its mobility, smaller fragments end up nearer to the anode than longer ones in a given period. After some time, the voltage is removed and the fragmentation gradient is analyzed. For larger separations between similar sized fragments, either the voltage or run time can be increased. Extended runs across a low voltage gel yield the most accurate resolution. Voltage is, however, not the sole factor in determining electrophoresis of nucleic acids.The nucleic acid to be separated can be prepared in several ways before separation by electrophoresis. In the case of large DNA molecules, the DNA is frequently cut into smaller fragments using a DNA restriction endonuclease (or restriction enzyme). In other instances, such as PCR amplified samples, enzymes present in the sample that might affect the separation of the molecules are removed through various means before analysis. Once the nucleic acid is properly prepared, the samples of the nucleic acid solution are placed in the wells of the gel and a voltage is applied across the gel for a specified amount of time.The DNA fragments of different lengths are visualized using a fluorescent dye specific for DNA, such as ethidium bromide. The gel shows bands corresponding to different nucleic acid molecules populations with different molecular weight. Fragment size is usually reported in ""nucleotides"", ""base pairs"" or ""kb"" (for thousands of base pairs) depending upon whether single- or double-stranded nucleic acid has been separated. Fragment size determination is typically done by comparison to commercially available DNA markers containing linear DNA fragments of known length.The types of gel most commonly used for nucleic acid electrophoresis are agarose (for relatively long DNA molecules) and polyacrylamide (for high resolution of short DNA molecules, for example in DNA sequencing). Gels have conventionally been run in a ""slab"" format such as that shown in the figure, but capillary electrophoresis has become important for applications such as high-throughput DNA sequencing. Electrophoresis techniques used in the assessment of DNA damage include alkaline gel electrophoresis and pulsed field gel electrophoresis.For short DNA segments such as 20 to 60 bp double stranded DNA, running them in Polyacrylamide gel (PAGE) will give better resolution(native condition). Similarly, RNA and single stranded DNA can be run and visualised by PAGE gels containing denaturing agents such as Urea. PAGE gels are widely used in techniques such as DNA foot printing, EMSA and other DNA-protein interaction techniques.The measurement and analysis are mostly done with a specialized gel analysis software. Capillary electrophoresis results are typically displayed in a trace view called an electropherogram.