Ch 16-17 Practice Quiz
... 3. Which of the following is the actual statement that describes how DNA replicates? a. semi conservative b. dispersive c. conservative 4. Put these events in the correct chronological order: • Chargaff–base pairing (A-T, C-G) • Meselson-Stahl –DNA Replication details • Watson and Crick (discovered ...
... 3. Which of the following is the actual statement that describes how DNA replicates? a. semi conservative b. dispersive c. conservative 4. Put these events in the correct chronological order: • Chargaff–base pairing (A-T, C-G) • Meselson-Stahl –DNA Replication details • Watson and Crick (discovered ...
Researchers ACT on DNA Storage
... Shakespeare's sonnets and an audio recording of Martin Luther King. The work is published in the journal Nature. [Nick Goldman et al, Towards practical, high-capacity, low-maintenance information storage in synthesized DNA] Unlike many forms of information storage, DNA is extremely long-lasting and ...
... Shakespeare's sonnets and an audio recording of Martin Luther King. The work is published in the journal Nature. [Nick Goldman et al, Towards practical, high-capacity, low-maintenance information storage in synthesized DNA] Unlike many forms of information storage, DNA is extremely long-lasting and ...
Slide 1
... sugar and phosphate groups make up the uprights of the ladder and the rungs are nitrogen bases hydrogen bonded together (recall hydrogen bonds are weak bonds and can come apart easily). The rungs are always one purine bonded to one pyrimidine…A always to T and C always to G. ...
... sugar and phosphate groups make up the uprights of the ladder and the rungs are nitrogen bases hydrogen bonded together (recall hydrogen bonds are weak bonds and can come apart easily). The rungs are always one purine bonded to one pyrimidine…A always to T and C always to G. ...
Lab23
... Add dye to PCR samples and load as much of each sample as will fit in the well into the good experimental gel: no air bubbles in tip while loading! Be certain power source is set to 70 VOLTS not AMPS Run gels for ~1.5 hrs (have lecture while waiting) Stain and destain gel Interpret results ...
... Add dye to PCR samples and load as much of each sample as will fit in the well into the good experimental gel: no air bubbles in tip while loading! Be certain power source is set to 70 VOLTS not AMPS Run gels for ~1.5 hrs (have lecture while waiting) Stain and destain gel Interpret results ...
genetics science learning center – internet lesson
... Site Location: http://gslc.genetics.utah.edu/ Familiarize yourself with the homepage of this site. Click on the link that says “Tour the basics”. A new window will open, you can navigate sections by using the top toolbar. WHAT IS DNA? 1. What does DNA stand for? 2. Why is DNA called a blueprint? 3. ...
... Site Location: http://gslc.genetics.utah.edu/ Familiarize yourself with the homepage of this site. Click on the link that says “Tour the basics”. A new window will open, you can navigate sections by using the top toolbar. WHAT IS DNA? 1. What does DNA stand for? 2. Why is DNA called a blueprint? 3. ...
suggested essay-type questions for next exam
... pairs, thereby unwinding the supercoils. However, the linking number of the DNA is not changed! Explain the physical basis for the ability of ethidium bromide to “unwind” these supercoils. (You will have to look at the definition of the linking difference. In this definition, Lo refers to the linkin ...
... pairs, thereby unwinding the supercoils. However, the linking number of the DNA is not changed! Explain the physical basis for the ability of ethidium bromide to “unwind” these supercoils. (You will have to look at the definition of the linking difference. In this definition, Lo refers to the linkin ...
Name
... 5. Label the positive and negative ends of the electrophoresis gel and place the DNA segments for each clone number in order from smallest to largest. (2) ...
... 5. Label the positive and negative ends of the electrophoresis gel and place the DNA segments for each clone number in order from smallest to largest. (2) ...
File - Chereese Langley
... helix is in the DNA molecule. The double helix is a pair of parallel helices intertwined about a common axis. Adenine, thymine, cytosine, and guanine are a compound that is one of the four constituent bases of nucleic acid which are all apart of this DNA molecule. The guanine occurs in guano and fis ...
... helix is in the DNA molecule. The double helix is a pair of parallel helices intertwined about a common axis. Adenine, thymine, cytosine, and guanine are a compound that is one of the four constituent bases of nucleic acid which are all apart of this DNA molecule. The guanine occurs in guano and fis ...
Chapter 13 Genetic Engineering
... • Gel Electrophoresis- DNA Fragments are placed in certain gel wells and an electric voltage is passed through them. • DNA molecules move toward the opposite end of the gel. • Smaller DNA fragments move faster through the gel. ...
... • Gel Electrophoresis- DNA Fragments are placed in certain gel wells and an electric voltage is passed through them. • DNA molecules move toward the opposite end of the gel. • Smaller DNA fragments move faster through the gel. ...
File - Biology with Radjewski
... o On vs. off o What types of cells have this? o Role of lactose (or allolactose) Lac operon vs. trp operon Genetic Engineering (5 m/c + plasmid mapping) Restriction Enyzmes Sticky ends Hydrogen bonds DNA charge Direction DNA migrates in gel electrophoresis Which sized DNA fragments mov ...
... o On vs. off o What types of cells have this? o Role of lactose (or allolactose) Lac operon vs. trp operon Genetic Engineering (5 m/c + plasmid mapping) Restriction Enyzmes Sticky ends Hydrogen bonds DNA charge Direction DNA migrates in gel electrophoresis Which sized DNA fragments mov ...
My Dinosaur
... • The original DNA is isolated and cut by restriction enzymes in specific areas • This procedure uses staggered cuts ...
... • The original DNA is isolated and cut by restriction enzymes in specific areas • This procedure uses staggered cuts ...
Bio Quiz #4 Review Sheet
... ___A DNA sequence that acts as a carrier for a transgene ___A technique uses an agarsoe gel and electrical current to separates DNA fragments by size ___An organism that has DNA from two or more sources ___A protein that is used to cut DNA into fragments ___A sequence of DNA from multiple sources __ ...
... ___A DNA sequence that acts as a carrier for a transgene ___A technique uses an agarsoe gel and electrical current to separates DNA fragments by size ___An organism that has DNA from two or more sources ___A protein that is used to cut DNA into fragments ___A sequence of DNA from multiple sources __ ...
2.5.4. DNA Revision Qs
... 3 Say if the following variations are inherited or acquired. (a) freckles _____________________________________ (b) the production of an enzyme _____________________________________ (c) the ability to play a musical instrument _____________________________________ (d) the ability to form a blood clo ...
... 3 Say if the following variations are inherited or acquired. (a) freckles _____________________________________ (b) the production of an enzyme _____________________________________ (c) the ability to play a musical instrument _____________________________________ (d) the ability to form a blood clo ...
DNA Technology
... 1. Explain the technique. Be specific and brief (one paragraph) List your source. 2. Research an example of how the technique has been used by humans. You can use one of the examples listed above or find your own. Be specific in explaining how the technique was used. Cite your sources – not the text ...
... 1. Explain the technique. Be specific and brief (one paragraph) List your source. 2. Research an example of how the technique has been used by humans. You can use one of the examples listed above or find your own. Be specific in explaining how the technique was used. Cite your sources – not the text ...
Name Date Period BioTechnology: Web Quest Part 1
... Read the introduction and the 3 main points of gel electrophoresis. Run the animations. 1. The DNA is being cut into fragments by __________________________________ 2. Where are the fragments transferred? ___________________________________ 3. What goes through the gel that creates a negative charge ...
... Read the introduction and the 3 main points of gel electrophoresis. Run the animations. 1. The DNA is being cut into fragments by __________________________________ 2. Where are the fragments transferred? ___________________________________ 3. What goes through the gel that creates a negative charge ...
Genetics - Bill Nye ANSWERS
... DNA is responsible for making proteins. RNA is similar to DNA, but its different. What’s different? RNA only has one strand. There are 20 amino acids that make up proteins. Name the 2 scientists that discovered the double helix. Watson and Crick How many bases align in a sequence to code for a speci ...
... DNA is responsible for making proteins. RNA is similar to DNA, but its different. What’s different? RNA only has one strand. There are 20 amino acids that make up proteins. Name the 2 scientists that discovered the double helix. Watson and Crick How many bases align in a sequence to code for a speci ...
Chapter 10 Section 3 Notes Answer Key
... I. DNA A. DNA- a chemical that contains information an organism needs to grow and function 1. Watson and Crick made and accurate model of DNA in 1953 2. The structure of DNA is similar to a twisted ladder. a. The sides of the ladder are made up of sugarphosphate molecules. b. The rungs of the ladder ...
... I. DNA A. DNA- a chemical that contains information an organism needs to grow and function 1. Watson and Crick made and accurate model of DNA in 1953 2. The structure of DNA is similar to a twisted ladder. a. The sides of the ladder are made up of sugarphosphate molecules. b. The rungs of the ladder ...
Chapter 12 DNA Analysis Checkpoint Answers In the nucleus of the
... 6. Adenine, guanine, cytosine, thymine 7. The phosphate groups give DNA its acidic properties. 8. Blood, semen, saliva, hair follicular tissue, bone 9. Restriction Fragment Length Polymorphism ...
... 6. Adenine, guanine, cytosine, thymine 7. The phosphate groups give DNA its acidic properties. 8. Blood, semen, saliva, hair follicular tissue, bone 9. Restriction Fragment Length Polymorphism ...
The Genetic Code
... • The sequence of the nitrogen bases form the code. Certain combinations of three bases will code for a specific amino acid. Amino acids link together to form a protein. ...
... • The sequence of the nitrogen bases form the code. Certain combinations of three bases will code for a specific amino acid. Amino acids link together to form a protein. ...
SW describe how techniques such as DNA
... differently in the two sexes. Such traits are autosomal, which means that the genes responsible for their expression are not carried on the sex chromosomes. ...
... differently in the two sexes. Such traits are autosomal, which means that the genes responsible for their expression are not carried on the sex chromosomes. ...
Genetics 1. What do the letters DNA stand for? 2. Two scientists are
... 11. Based on this information, scientist could predict that the base _______________________ pairs with _______________________ and the base _______________________ pairs with ___________________ ____ in the formation of the DNA molecule.This is called complementary base pairs. Thus one strand of DN ...
... 11. Based on this information, scientist could predict that the base _______________________ pairs with _______________________ and the base _______________________ pairs with ___________________ ____ in the formation of the DNA molecule.This is called complementary base pairs. Thus one strand of DN ...
Gel electrophoresis of nucleic acids
Nucleic acid electrophoresis is an analytical technique used to separate DNA or RNA fragments by size and reactivity. Nucleic acid molecules which are to be analyzed are set upon a viscous medium, the gel, where an electric field induces the nucleic acids to migrate toward the anode, due to the net negative charge of the sugar-phosphate backbone of the nucleic acid chain. The separation of these fragments is accomplished by exploiting the mobilities with which different sized molecules are able to pass through the gel. Longer molecules migrate more slowly because they experience more resistance within the gel. Because the size of the molecule affects its mobility, smaller fragments end up nearer to the anode than longer ones in a given period. After some time, the voltage is removed and the fragmentation gradient is analyzed. For larger separations between similar sized fragments, either the voltage or run time can be increased. Extended runs across a low voltage gel yield the most accurate resolution. Voltage is, however, not the sole factor in determining electrophoresis of nucleic acids.The nucleic acid to be separated can be prepared in several ways before separation by electrophoresis. In the case of large DNA molecules, the DNA is frequently cut into smaller fragments using a DNA restriction endonuclease (or restriction enzyme). In other instances, such as PCR amplified samples, enzymes present in the sample that might affect the separation of the molecules are removed through various means before analysis. Once the nucleic acid is properly prepared, the samples of the nucleic acid solution are placed in the wells of the gel and a voltage is applied across the gel for a specified amount of time.The DNA fragments of different lengths are visualized using a fluorescent dye specific for DNA, such as ethidium bromide. The gel shows bands corresponding to different nucleic acid molecules populations with different molecular weight. Fragment size is usually reported in ""nucleotides"", ""base pairs"" or ""kb"" (for thousands of base pairs) depending upon whether single- or double-stranded nucleic acid has been separated. Fragment size determination is typically done by comparison to commercially available DNA markers containing linear DNA fragments of known length.The types of gel most commonly used for nucleic acid electrophoresis are agarose (for relatively long DNA molecules) and polyacrylamide (for high resolution of short DNA molecules, for example in DNA sequencing). Gels have conventionally been run in a ""slab"" format such as that shown in the figure, but capillary electrophoresis has become important for applications such as high-throughput DNA sequencing. Electrophoresis techniques used in the assessment of DNA damage include alkaline gel electrophoresis and pulsed field gel electrophoresis.For short DNA segments such as 20 to 60 bp double stranded DNA, running them in Polyacrylamide gel (PAGE) will give better resolution(native condition). Similarly, RNA and single stranded DNA can be run and visualised by PAGE gels containing denaturing agents such as Urea. PAGE gels are widely used in techniques such as DNA foot printing, EMSA and other DNA-protein interaction techniques.The measurement and analysis are mostly done with a specialized gel analysis software. Capillary electrophoresis results are typically displayed in a trace view called an electropherogram.