Locard Exchange Principle
... ______ provides a powerful technique for uniquely identifying the person or animal who left traces of body fluids at a crime scene. Indeed, this is the best method presently known for such identification DNA _________________: the process that separates DNA using gel and electricity DNA ___________ ...
... ______ provides a powerful technique for uniquely identifying the person or animal who left traces of body fluids at a crime scene. Indeed, this is the best method presently known for such identification DNA _________________: the process that separates DNA using gel and electricity DNA ___________ ...
Genetic Disorders
... -symptoms include tall stature, and abnormal body proportions, may be infertile, have feminine features and less developed male features ...
... -symptoms include tall stature, and abnormal body proportions, may be infertile, have feminine features and less developed male features ...
All Living things pass on their genetic heritage by common processes.
... George Beadle and Edward Tatum (late 40’s to early 50’s) used X-rays to induce mutations in Neurospora crassa, which were unable to synthesize amino acid and vitamins. They traced the defect to the enzymes involved in their synthesis. 2 Hershey-Chase (1952) experiment extended Avery, Macleod and McC ...
... George Beadle and Edward Tatum (late 40’s to early 50’s) used X-rays to induce mutations in Neurospora crassa, which were unable to synthesize amino acid and vitamins. They traced the defect to the enzymes involved in their synthesis. 2 Hershey-Chase (1952) experiment extended Avery, Macleod and McC ...
Guanine – Cytosine
... If you unraveled all your chromosomes from all of your cells and laid out the DNA end to end, the strands would stretch from the Earth to the Moon about 6,000 times. ...
... If you unraveled all your chromosomes from all of your cells and laid out the DNA end to end, the strands would stretch from the Earth to the Moon about 6,000 times. ...
Genetic Engineering
... 2. Cross-breeding / Hybridization – cross two different types of individuals to get the best characteristics of both. ...
... 2. Cross-breeding / Hybridization – cross two different types of individuals to get the best characteristics of both. ...
Name_____________________ Date__________ Class
... organism whose genome has been engineered in the laboratory in order to favor the expression of desired physiological traits or the production of desired biological products a technique for separating protein molecules of varying sizes in a mixture by moving them through a block of gel, by means of ...
... organism whose genome has been engineered in the laboratory in order to favor the expression of desired physiological traits or the production of desired biological products a technique for separating protein molecules of varying sizes in a mixture by moving them through a block of gel, by means of ...
Genetic Engineering pp 2014
... 1. Cut DNA with restriction enzymes 2. Mix DNA samples with sugar so they will sink 3. Inject the samples into the wells 4. Turn on the power and the DNA will travel across the gel. *the smallest fragments will travel the farthest. ...
... 1. Cut DNA with restriction enzymes 2. Mix DNA samples with sugar so they will sink 3. Inject the samples into the wells 4. Turn on the power and the DNA will travel across the gel. *the smallest fragments will travel the farthest. ...
DNA Paternity Test RFLP analysis (Restriction Fragment Length
... -RFLP analysis targets DNA segments known to have variability between individuals and cuts with enzymes that show those differences -cut DNA fragments are then analyzed on agarose gel electrophoresis ...
... -RFLP analysis targets DNA segments known to have variability between individuals and cuts with enzymes that show those differences -cut DNA fragments are then analyzed on agarose gel electrophoresis ...
What are the three steps in PCR?
... It is often used in DNA fingerprinting It requires gel electrophoresis which separates DNA by size ...
... It is often used in DNA fingerprinting It requires gel electrophoresis which separates DNA by size ...
Unit 4 Review Sheet Genetics and Biotechnology Vocabulary
... - Do you know how to use the codon chart? - Why is the sequence of amino acids important to the shape and function of a protein? *You do NOT need to know the names of the enzymes involved in this process. Mutations - What is a mutation? - What kind of mutations can happen to DNA (i.e. a nucleotide i ...
... - Do you know how to use the codon chart? - Why is the sequence of amino acids important to the shape and function of a protein? *You do NOT need to know the names of the enzymes involved in this process. Mutations - What is a mutation? - What kind of mutations can happen to DNA (i.e. a nucleotide i ...
Document
... passed between wire electrodes at each end of the chamber. Since DNA fragments are negatively charged, they will be drawn toward the positive pole (anode) when placed in an electric field. The matrix of the agarose gel acts as a molecular sieve through which smaller DNA fragments can move more easil ...
... passed between wire electrodes at each end of the chamber. Since DNA fragments are negatively charged, they will be drawn toward the positive pole (anode) when placed in an electric field. The matrix of the agarose gel acts as a molecular sieve through which smaller DNA fragments can move more easil ...
GENETIC ENGINEERING QUESTIONS
... 1. A scientist wants to mass produce a recombinant form of a protein for human use. He should first a. Purify the protein b. Clone the gene for the protein c. Perform PCR on the protein d. Grow bacteria to make the protein 2. People show restriction fragment length polymorphism because a. They have ...
... 1. A scientist wants to mass produce a recombinant form of a protein for human use. He should first a. Purify the protein b. Clone the gene for the protein c. Perform PCR on the protein d. Grow bacteria to make the protein 2. People show restriction fragment length polymorphism because a. They have ...
WINK DNA Structure and Replication
... and chromosomes in coding the instructions for characteristic traits transferred from parent to offspring. * Develop and use models to explain how genetic information (DNA) is copied for transmission to subsequent generations of cells (mitosis). ...
... and chromosomes in coding the instructions for characteristic traits transferred from parent to offspring. * Develop and use models to explain how genetic information (DNA) is copied for transmission to subsequent generations of cells (mitosis). ...
Manipulating DNA - Lemon Bay High School
... • The joining together of genetic material from two (or more) different organisms. • Transgenic Microorganisms are Bacteria transformed with the genes for human proteins These now produce important compounds cheaply and in great abundance: Insulin, growth hormone, and clotting factor ...
... • The joining together of genetic material from two (or more) different organisms. • Transgenic Microorganisms are Bacteria transformed with the genes for human proteins These now produce important compounds cheaply and in great abundance: Insulin, growth hormone, and clotting factor ...
DNA - VanityWolveriine
... DNA exactly how it is. Sometimes if it doesn’t copy correctly, the cell either dies or a mutation occurs. ...
... DNA exactly how it is. Sometimes if it doesn’t copy correctly, the cell either dies or a mutation occurs. ...
Molecular Basis of Inheritance
... Details Of The Structure • DNA is formed from two nucleotide polymers each with covalent bonds between the sugar and phosphate groups (backbone structure) and variable nucleotide bases capable of Hydrogen bonding Conserved region ...
... Details Of The Structure • DNA is formed from two nucleotide polymers each with covalent bonds between the sugar and phosphate groups (backbone structure) and variable nucleotide bases capable of Hydrogen bonding Conserved region ...
The Human Genome Project CH 13 Sec 3 notes
... •Discover structure and function of proteins •Compare similar proteins from different organisms –Group protein sequences into families ...
... •Discover structure and function of proteins •Compare similar proteins from different organisms –Group protein sequences into families ...
Gel electrophoresis of nucleic acids
Nucleic acid electrophoresis is an analytical technique used to separate DNA or RNA fragments by size and reactivity. Nucleic acid molecules which are to be analyzed are set upon a viscous medium, the gel, where an electric field induces the nucleic acids to migrate toward the anode, due to the net negative charge of the sugar-phosphate backbone of the nucleic acid chain. The separation of these fragments is accomplished by exploiting the mobilities with which different sized molecules are able to pass through the gel. Longer molecules migrate more slowly because they experience more resistance within the gel. Because the size of the molecule affects its mobility, smaller fragments end up nearer to the anode than longer ones in a given period. After some time, the voltage is removed and the fragmentation gradient is analyzed. For larger separations between similar sized fragments, either the voltage or run time can be increased. Extended runs across a low voltage gel yield the most accurate resolution. Voltage is, however, not the sole factor in determining electrophoresis of nucleic acids.The nucleic acid to be separated can be prepared in several ways before separation by electrophoresis. In the case of large DNA molecules, the DNA is frequently cut into smaller fragments using a DNA restriction endonuclease (or restriction enzyme). In other instances, such as PCR amplified samples, enzymes present in the sample that might affect the separation of the molecules are removed through various means before analysis. Once the nucleic acid is properly prepared, the samples of the nucleic acid solution are placed in the wells of the gel and a voltage is applied across the gel for a specified amount of time.The DNA fragments of different lengths are visualized using a fluorescent dye specific for DNA, such as ethidium bromide. The gel shows bands corresponding to different nucleic acid molecules populations with different molecular weight. Fragment size is usually reported in ""nucleotides"", ""base pairs"" or ""kb"" (for thousands of base pairs) depending upon whether single- or double-stranded nucleic acid has been separated. Fragment size determination is typically done by comparison to commercially available DNA markers containing linear DNA fragments of known length.The types of gel most commonly used for nucleic acid electrophoresis are agarose (for relatively long DNA molecules) and polyacrylamide (for high resolution of short DNA molecules, for example in DNA sequencing). Gels have conventionally been run in a ""slab"" format such as that shown in the figure, but capillary electrophoresis has become important for applications such as high-throughput DNA sequencing. Electrophoresis techniques used in the assessment of DNA damage include alkaline gel electrophoresis and pulsed field gel electrophoresis.For short DNA segments such as 20 to 60 bp double stranded DNA, running them in Polyacrylamide gel (PAGE) will give better resolution(native condition). Similarly, RNA and single stranded DNA can be run and visualised by PAGE gels containing denaturing agents such as Urea. PAGE gels are widely used in techniques such as DNA foot printing, EMSA and other DNA-protein interaction techniques.The measurement and analysis are mostly done with a specialized gel analysis software. Capillary electrophoresis results are typically displayed in a trace view called an electropherogram.