Genetic Engineering Powerpoint
... DNA molecules too large to work with Can be cut up using Restriction Enzymes They cut DNA at specific nucleotide ...
... DNA molecules too large to work with Can be cut up using Restriction Enzymes They cut DNA at specific nucleotide ...
WS 12 - Department of Chemistry | Oregon State University
... model is due to hydrogen bonding and __________. ...
... model is due to hydrogen bonding and __________. ...
Chapter 13 Genetic Engineering Changing the living world
... Gel Electrophoresis •Enzymes cut DNA into fragments •DNA fragments are poured onto a gel •Electric voltage moves the DNA fragments across the gel •Because longer segments move across the gel more slowly, and do not go as far •Based on size, the DNA fragments make a pattern of bands on the gel ...
... Gel Electrophoresis •Enzymes cut DNA into fragments •DNA fragments are poured onto a gel •Electric voltage moves the DNA fragments across the gel •Because longer segments move across the gel more slowly, and do not go as far •Based on size, the DNA fragments make a pattern of bands on the gel ...
Nucleic Acids - faculty at Chemeketa
... What will be the composition of the DNA strand complementary to –AGCCA– ? a. b. c. d. ...
... What will be the composition of the DNA strand complementary to –AGCCA– ? a. b. c. d. ...
Bio07_TR__U04_CH13.QXD
... 2. Crossing dissimilar individuals to bring together the best of both Organisms is called ________________________ . 3. The continued breeding of individuals with similar characteristics is called _______________________ . 4. Biologists change the DNA code of a living organism through ______________ ...
... 2. Crossing dissimilar individuals to bring together the best of both Organisms is called ________________________ . 3. The continued breeding of individuals with similar characteristics is called _______________________ . 4. Biologists change the DNA code of a living organism through ______________ ...
Evidence of Evolution - David Brotherton CCCMC
... Gel Electrophoresis: Process that allows scientists to compare DNA from different organisms. DNA fingerprint: A pattern produced by gel electrophoresis. The more similar the pattern, the more related the organisms. Steps: 1. DNA is cut into fragments of different sizes. 2. DNA is placed in the we ...
... Gel Electrophoresis: Process that allows scientists to compare DNA from different organisms. DNA fingerprint: A pattern produced by gel electrophoresis. The more similar the pattern, the more related the organisms. Steps: 1. DNA is cut into fragments of different sizes. 2. DNA is placed in the we ...
exam 2 summary
... >buffer vials, two electrodes connected to a power source, a laser >excitation source, fluorescence detectors, a auto sampler to hold sample >vials and a computer component that controls sample injection and >detection. The capillary is made of fused silica and contains gel used to >separate the DNA ...
... >buffer vials, two electrodes connected to a power source, a laser >excitation source, fluorescence detectors, a auto sampler to hold sample >vials and a computer component that controls sample injection and >detection. The capillary is made of fused silica and contains gel used to >separate the DNA ...
DNA the Molecule of molecules - Foothill Technology High
... b. In human cells, it takes only a few hours to copy the 6 billion bases. 2. Accurate Only about 1 in a billion base pairs is incorrectly paired. ...
... b. In human cells, it takes only a few hours to copy the 6 billion bases. 2. Accurate Only about 1 in a billion base pairs is incorrectly paired. ...
DNA Fingerprinting
... II. How is it used to solve crimes? A. Everywhere you go, you shed cells B. At crime scenes, investigators can look for them 1. skin 2. hair 3. blood 4. any body fluids ...
... II. How is it used to solve crimes? A. Everywhere you go, you shed cells B. At crime scenes, investigators can look for them 1. skin 2. hair 3. blood 4. any body fluids ...
Aim: What are some techniques used in DNA engineering?
... Gel electrophoresis separates macromolecules - nucleic acids or proteins - on the basis of their rate of movement through a gel in an electrical field For linear DNA molecules, separation depends mainly on size (length of fragment) with longer fragments migrating less along the gel. After treating l ...
... Gel electrophoresis separates macromolecules - nucleic acids or proteins - on the basis of their rate of movement through a gel in an electrical field For linear DNA molecules, separation depends mainly on size (length of fragment) with longer fragments migrating less along the gel. After treating l ...
Student Handout - University of California, Irvine
... As the pieces of DNA move through the gel matrix, they will meet resistance. ____________ pieces of DNA will have more difficulty moving through the gel than ___________ fragments. Thus, larger fragments will move _____________ than smaller fragments. This allows separation of all different ______ ...
... As the pieces of DNA move through the gel matrix, they will meet resistance. ____________ pieces of DNA will have more difficulty moving through the gel than ___________ fragments. Thus, larger fragments will move _____________ than smaller fragments. This allows separation of all different ______ ...
DNA and Chromosomes
... What is the relationship between DNA, chromosomes, and any organism? Drag and drop the descriptive phrase to the correct column, thereby helping us to describe the relationships between these important components of inheritance. ...
... What is the relationship between DNA, chromosomes, and any organism? Drag and drop the descriptive phrase to the correct column, thereby helping us to describe the relationships between these important components of inheritance. ...
Application Sheet: DNA - NETZSCH Thermal Analysis
... NGB ∙ AS ∙ 135-2006 ∙ EN ∙ 07/13 ∙ Technical specifications are subject to change. ...
... NGB ∙ AS ∙ 135-2006 ∙ EN ∙ 07/13 ∙ Technical specifications are subject to change. ...
Prediction practice - unlinked
... Whether the end of the stem is left in the air to callus over night How many leaves are on the cutting Whether the cutting is placed in compost or water Temperature of the environment surrounding the cutting Whether a plastic bag is covering the cutting If there are holes in the plastic bag over the ...
... Whether the end of the stem is left in the air to callus over night How many leaves are on the cutting Whether the cutting is placed in compost or water Temperature of the environment surrounding the cutting Whether a plastic bag is covering the cutting If there are holes in the plastic bag over the ...
Gel electrophoresis of nucleic acids
Nucleic acid electrophoresis is an analytical technique used to separate DNA or RNA fragments by size and reactivity. Nucleic acid molecules which are to be analyzed are set upon a viscous medium, the gel, where an electric field induces the nucleic acids to migrate toward the anode, due to the net negative charge of the sugar-phosphate backbone of the nucleic acid chain. The separation of these fragments is accomplished by exploiting the mobilities with which different sized molecules are able to pass through the gel. Longer molecules migrate more slowly because they experience more resistance within the gel. Because the size of the molecule affects its mobility, smaller fragments end up nearer to the anode than longer ones in a given period. After some time, the voltage is removed and the fragmentation gradient is analyzed. For larger separations between similar sized fragments, either the voltage or run time can be increased. Extended runs across a low voltage gel yield the most accurate resolution. Voltage is, however, not the sole factor in determining electrophoresis of nucleic acids.The nucleic acid to be separated can be prepared in several ways before separation by electrophoresis. In the case of large DNA molecules, the DNA is frequently cut into smaller fragments using a DNA restriction endonuclease (or restriction enzyme). In other instances, such as PCR amplified samples, enzymes present in the sample that might affect the separation of the molecules are removed through various means before analysis. Once the nucleic acid is properly prepared, the samples of the nucleic acid solution are placed in the wells of the gel and a voltage is applied across the gel for a specified amount of time.The DNA fragments of different lengths are visualized using a fluorescent dye specific for DNA, such as ethidium bromide. The gel shows bands corresponding to different nucleic acid molecules populations with different molecular weight. Fragment size is usually reported in ""nucleotides"", ""base pairs"" or ""kb"" (for thousands of base pairs) depending upon whether single- or double-stranded nucleic acid has been separated. Fragment size determination is typically done by comparison to commercially available DNA markers containing linear DNA fragments of known length.The types of gel most commonly used for nucleic acid electrophoresis are agarose (for relatively long DNA molecules) and polyacrylamide (for high resolution of short DNA molecules, for example in DNA sequencing). Gels have conventionally been run in a ""slab"" format such as that shown in the figure, but capillary electrophoresis has become important for applications such as high-throughput DNA sequencing. Electrophoresis techniques used in the assessment of DNA damage include alkaline gel electrophoresis and pulsed field gel electrophoresis.For short DNA segments such as 20 to 60 bp double stranded DNA, running them in Polyacrylamide gel (PAGE) will give better resolution(native condition). Similarly, RNA and single stranded DNA can be run and visualised by PAGE gels containing denaturing agents such as Urea. PAGE gels are widely used in techniques such as DNA foot printing, EMSA and other DNA-protein interaction techniques.The measurement and analysis are mostly done with a specialized gel analysis software. Capillary electrophoresis results are typically displayed in a trace view called an electropherogram.