So You Think
... won the Nobel Prize for discovering the shape of DNA. ________________ 5. DNA is said to have a ___________ ___________ ________________ shape. ________________ 6. Weak _________________ bonds allow the DNA ________________ molecule to “unzip”. ________________ 7. RNA contains three of the same nucl ...
... won the Nobel Prize for discovering the shape of DNA. ________________ 5. DNA is said to have a ___________ ___________ ________________ shape. ________________ 6. Weak _________________ bonds allow the DNA ________________ molecule to “unzip”. ________________ 7. RNA contains three of the same nucl ...
a10c Biotechnology
... in what they cleave? What do they "look for"? Name an example of a restriction enzyme. 3. Describe the steps of cloning (transferring a gene to bacteria for purposes of "growing" DNA or protein). What enzymes are used in the process? What form of bacterial gene transfer is used in the lab to facilit ...
... in what they cleave? What do they "look for"? Name an example of a restriction enzyme. 3. Describe the steps of cloning (transferring a gene to bacteria for purposes of "growing" DNA or protein). What enzymes are used in the process? What form of bacterial gene transfer is used in the lab to facilit ...
Let`s Find the Pheromone Gene
... 1. Get pre-poured gels and remove tape 2. Using pipettor, fill wells with 5uL of Head, Thorax, and Abdomen PCR products as well as the controls and the ladder 3. Molecular Technician puts gel in the buffer-filled box and starts the electrical charge (RUN TO RED! DNA is negative and runs to the posit ...
... 1. Get pre-poured gels and remove tape 2. Using pipettor, fill wells with 5uL of Head, Thorax, and Abdomen PCR products as well as the controls and the ladder 3. Molecular Technician puts gel in the buffer-filled box and starts the electrical charge (RUN TO RED! DNA is negative and runs to the posit ...
DNA-Polymerase
... of injecting DNA into agarose gel and then applying an electric current to the gel. As a result, the smaller DNA strands move faster than the larger strands through the gel toward the positive current. The size of the PCR product can be determined by comparing it with a DNA ladder, which contains DN ...
... of injecting DNA into agarose gel and then applying an electric current to the gel. As a result, the smaller DNA strands move faster than the larger strands through the gel toward the positive current. The size of the PCR product can be determined by comparing it with a DNA ladder, which contains DN ...
Introduction to Biotechnology Gel Electrophoresis and DNA Analysis
... 2. If you were to use a 4% agarose gel instead of a .8% agarose gel, what effects would this make on the electrophoresis gel and why? Giver three examples, ideas. The fragments would move less in 4% gel as increasing % means more “mesh” (agarose) which slows DNA. Tighter bands @ 4%, the tighter mesh ...
... 2. If you were to use a 4% agarose gel instead of a .8% agarose gel, what effects would this make on the electrophoresis gel and why? Giver three examples, ideas. The fragments would move less in 4% gel as increasing % means more “mesh” (agarose) which slows DNA. Tighter bands @ 4%, the tighter mesh ...
CH-13 Sect 1
... 24. What is a transgenic organism? ___________________________________________________________________________ 25. What is a clone? ___________________________________________________________________________ 26. List four ways in which transgenic animals have been used. a. __________________________ ...
... 24. What is a transgenic organism? ___________________________________________________________________________ 25. What is a clone? ___________________________________________________________________________ 26. List four ways in which transgenic animals have been used. a. __________________________ ...
DNA, Genes and Chromosomes
... 2. To obtain a merit you need to use pictures of your DNA model in a flow diagram showing the progression from a cell to a gene writing descriptions. 3. A distinction will be achieved if you produce a poster writing a summary about how genes can be shuffled ...
... 2. To obtain a merit you need to use pictures of your DNA model in a flow diagram showing the progression from a cell to a gene writing descriptions. 3. A distinction will be achieved if you produce a poster writing a summary about how genes can be shuffled ...
Bio 313 worksheet 2 - Iowa State University
... technique called electrophoresis. With this technique, DNA molecules are placed in a gel, an electrical current is applied to the gel, and the DNA molecules migrate toward the positive pole of the current. What aspect of its structure causes a DNA molecule to migrate toward the positive pole? ...
... technique called electrophoresis. With this technique, DNA molecules are placed in a gel, an electrical current is applied to the gel, and the DNA molecules migrate toward the positive pole of the current. What aspect of its structure causes a DNA molecule to migrate toward the positive pole? ...
DNA Fingerprinting Notes - Hicksville Public Schools
... ------------------------------------------------------------------------------------------------------------------------------1. Base your answer to the question on the diagram below and on your knowledge of biology. The diagram shows the results of a technique used to analyze DNA. This laboratory t ...
... ------------------------------------------------------------------------------------------------------------------------------1. Base your answer to the question on the diagram below and on your knowledge of biology. The diagram shows the results of a technique used to analyze DNA. This laboratory t ...
AZBio Ch 13
... Gel Electrophoresis •Enzymes cut DNA into fragments •DNA fragments are poured onto a gel •Electric voltage moves the DNA fragments across the gel •Because longer segments move across the gel more slowly, and do not go as far •Based on size, the DNA fragments make a pattern of bands on the gel ...
... Gel Electrophoresis •Enzymes cut DNA into fragments •DNA fragments are poured onto a gel •Electric voltage moves the DNA fragments across the gel •Because longer segments move across the gel more slowly, and do not go as far •Based on size, the DNA fragments make a pattern of bands on the gel ...
Biotech
... • This is the polymerase chain reaction. It is a technique to multiply a sample of DNA many times in a short period of time. It supplies the scientist with sufficient DNA for further testing. http://www.dnalc.org/resources/animations/pcr.html ...
... • This is the polymerase chain reaction. It is a technique to multiply a sample of DNA many times in a short period of time. It supplies the scientist with sufficient DNA for further testing. http://www.dnalc.org/resources/animations/pcr.html ...
13-2 Manipulating DNA
... have developed a series of tools that allow them to extract, edit, and then reinsert DNA into living organisms. ...
... have developed a series of tools that allow them to extract, edit, and then reinsert DNA into living organisms. ...
Document
... •DNA has a negative charge on its particles. • Molecules sort based on: •Charge - The greater the charge the more pull. •Size – Bigger pieces are slower, smaller are faster. •Shape - Coiled is slower straight is faster. •The negatively charged particles move toward the positive electrode while the p ...
... •DNA has a negative charge on its particles. • Molecules sort based on: •Charge - The greater the charge the more pull. •Size – Bigger pieces are slower, smaller are faster. •Shape - Coiled is slower straight is faster. •The negatively charged particles move toward the positive electrode while the p ...
Non-Mendelian Genetics Test Review
... pairs so that they may be visualized to determine abnormalities. ...
... pairs so that they may be visualized to determine abnormalities. ...
Recombinant DNA Technologies
... d. T- Thymine -put together in a double-helical molecule with A-T & C-G as the “rungs” -form GENES e. We have about 30,000 genes and they are mapped by location on each chromosome -”Human Genome Project” f. We are 99.9% identical; .1% makes us unique and different from Rob Marder (thank heaven!) a. ...
... d. T- Thymine -put together in a double-helical molecule with A-T & C-G as the “rungs” -form GENES e. We have about 30,000 genes and they are mapped by location on each chromosome -”Human Genome Project” f. We are 99.9% identical; .1% makes us unique and different from Rob Marder (thank heaven!) a. ...
pUC18 DNA HAE III Digest (D6293) - Datasheet - Sigma
... Bring the total volume to 7 µl with sterile water. 0.2–0.3 µg were loaded on a 10–20% acrylamide gradient gel. Gel electrophoresis was performed in 1× TBE (0.089 M Tris-borate, pH 8.3, 0.002 M EDTA). The gel was run with appropriate DNA fragment size standards at 70 volts until the tracking dye was ...
... Bring the total volume to 7 µl with sterile water. 0.2–0.3 µg were loaded on a 10–20% acrylamide gradient gel. Gel electrophoresis was performed in 1× TBE (0.089 M Tris-borate, pH 8.3, 0.002 M EDTA). The gel was run with appropriate DNA fragment size standards at 70 volts until the tracking dye was ...
Gel electrophoresis of nucleic acids
Nucleic acid electrophoresis is an analytical technique used to separate DNA or RNA fragments by size and reactivity. Nucleic acid molecules which are to be analyzed are set upon a viscous medium, the gel, where an electric field induces the nucleic acids to migrate toward the anode, due to the net negative charge of the sugar-phosphate backbone of the nucleic acid chain. The separation of these fragments is accomplished by exploiting the mobilities with which different sized molecules are able to pass through the gel. Longer molecules migrate more slowly because they experience more resistance within the gel. Because the size of the molecule affects its mobility, smaller fragments end up nearer to the anode than longer ones in a given period. After some time, the voltage is removed and the fragmentation gradient is analyzed. For larger separations between similar sized fragments, either the voltage or run time can be increased. Extended runs across a low voltage gel yield the most accurate resolution. Voltage is, however, not the sole factor in determining electrophoresis of nucleic acids.The nucleic acid to be separated can be prepared in several ways before separation by electrophoresis. In the case of large DNA molecules, the DNA is frequently cut into smaller fragments using a DNA restriction endonuclease (or restriction enzyme). In other instances, such as PCR amplified samples, enzymes present in the sample that might affect the separation of the molecules are removed through various means before analysis. Once the nucleic acid is properly prepared, the samples of the nucleic acid solution are placed in the wells of the gel and a voltage is applied across the gel for a specified amount of time.The DNA fragments of different lengths are visualized using a fluorescent dye specific for DNA, such as ethidium bromide. The gel shows bands corresponding to different nucleic acid molecules populations with different molecular weight. Fragment size is usually reported in ""nucleotides"", ""base pairs"" or ""kb"" (for thousands of base pairs) depending upon whether single- or double-stranded nucleic acid has been separated. Fragment size determination is typically done by comparison to commercially available DNA markers containing linear DNA fragments of known length.The types of gel most commonly used for nucleic acid electrophoresis are agarose (for relatively long DNA molecules) and polyacrylamide (for high resolution of short DNA molecules, for example in DNA sequencing). Gels have conventionally been run in a ""slab"" format such as that shown in the figure, but capillary electrophoresis has become important for applications such as high-throughput DNA sequencing. Electrophoresis techniques used in the assessment of DNA damage include alkaline gel electrophoresis and pulsed field gel electrophoresis.For short DNA segments such as 20 to 60 bp double stranded DNA, running them in Polyacrylamide gel (PAGE) will give better resolution(native condition). Similarly, RNA and single stranded DNA can be run and visualised by PAGE gels containing denaturing agents such as Urea. PAGE gels are widely used in techniques such as DNA foot printing, EMSA and other DNA-protein interaction techniques.The measurement and analysis are mostly done with a specialized gel analysis software. Capillary electrophoresis results are typically displayed in a trace view called an electropherogram.