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Enzymes - WordPress.com
Enzymes - WordPress.com

... something is. Proteins denature if the environment is too acid or too basic (3) ENZYME AND SUBSTRATE CONCENTRATION The rate of a chemical reaction is affected by the total number of enzymes as well as the concentration of substrates. (4) INHIBITORS: Inhibitors either slow down or stop the activity o ...
File
File

... that the substrate will “out compete” an inhibitor to bind with an enzyme? a. Probability increases because the concentration increased b. Probability stays the same since the number of enzymes doesn’t change c. Probability decreases because the concentration of substrate doesn’t play a role in its ...
Enzymes
Enzymes

... 2. Enzymes are catalysts that speed up reactions (they turn substrates into products) 3. Enzymes are specific (only 1 enzyme works for 1 substrate) 4. Enzymes do not get used up in the reaction 5. Enzymes only work at a specific temperature and pH. 6. Enzymes end in -ase ...
ENZYMES - Bio12.com
ENZYMES - Bio12.com

... The respiration pathway accelerates and ATP (the final product) builds up in the cell As the ATP increases, more and more ATP fits into the allosteric site of the phosphofructokinase molecules The enzyme’s conformation changes again and stops accepting substrate molecules in the active site Respirat ...
Enzymes
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... does it cause the reaction to occur. • A catalyst can increase the rate of a chemical reaction but cannot cause the reaction . ...
AP Lab 13: Enzyme Activity
AP Lab 13: Enzyme Activity

... AP Lab 13: Enzyme Activity Background: Enzymes are the catalysts of biological systems. They speed up chemical reactions in biological systems by lowering the activation energy, the energy needed for molecules to begin reacting with each other. Enzymes do this by forming an enzyme-substrate complex ...
Interesting facts: • During endocytosis, these intra
Interesting facts: • During endocytosis, these intra

... directly energized, ABC-type transporters (blue) of the plant vacuole. S, neutral solute; A–, anion; cat+, cation; X-conjugate, conjugate of a compound X (secondary metabolite or xenobiotic) with a hydrophilic compound such as glucose, glutathione, an amino acid, malonate, or sulphate. ...
scientific method
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... Joe baked a cake for his mother's birthday. When he removed the cake from the oven, Joe noticed that the cake had not risen. Joe guessed that the baking powder he had used was too old. He designed the following experiment to test his idea. Joe prepared two cakes – one using the same ingredients as h ...
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digestion - Manhasset Schools
digestion - Manhasset Schools

... A catalyst is any substance that increases the rate of a chemical reaction. Enzymes are specialized protein catalysts that control metabolic activities like hydrolysis (digestion), synthesis, cellular respiration and photosynthesis. Enzymes have specific shapes which allow them to bind to the reacta ...
Organic Chemistry Study Questions
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Enzyme Control of Metabolic Pathways

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Enzyme - CIE Alevel notes!
Enzyme - CIE Alevel notes!

... molecule in shape begin to break. This causes the tertiary structure of the enzyme to change, an effect called denaturation. This affects the shape of its active site. It becomes less likely that the substrate molecule will be able to bind with the enzyme, and the rate of reaction slows down. The te ...
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Enzyme Inhibition
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enzymes 2016
enzymes 2016

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Homeostasis Invertase
Homeostasis Invertase

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Enzyme kinetics



Enzyme kinetics is the study of the chemical reactions that are catalysed by enzymes. In enzyme kinetics, the reaction rate is measured and the effects of varying the conditions of the reaction are investigated. Studying an enzyme's kinetics in this way can reveal the catalytic mechanism of this enzyme, its role in metabolism, how its activity is controlled, and how a drug or an agonist might inhibit the enzyme.Enzymes are usually protein molecules that manipulate other molecules — the enzymes' substrates. These target molecules bind to an enzyme's active site and are transformed into products through a series of steps known as the enzymatic mechanismE + S <——> ES <——> ES*< ——> EP <——> E + P. These mechanisms can be divided into single-substrate and multiple-substrate mechanisms. Kinetic studies on enzymes that only bind one substrate, such as triosephosphate isomerase, aim to measure the affinity with which the enzyme binds this substrate and the turnover rate. Some other examples of enzymes are phosphofructokinase and hexokinase, both of which are important for cellular respiration (glycolysis).When enzymes bind multiple substrates, such as dihydrofolate reductase (shown right), enzyme kinetics can also show the sequence in which these substrates bind and the sequence in which products are released. An example of enzymes that bind a single substrate and release multiple products are proteases, which cleave one protein substrate into two polypeptide products. Others join two substrates together, such as DNA polymerase linking a nucleotide to DNA. Although these mechanisms are often a complex series of steps, there is typically one rate-determining step that determines the overall kinetics. This rate-determining step may be a chemical reaction or a conformational change of the enzyme or substrates, such as those involved in the release of product(s) from the enzyme.Knowledge of the enzyme's structure is helpful in interpreting kinetic data. For example, the structure can suggest how substrates and products bind during catalysis; what changes occur during the reaction; and even the role of particular amino acid residues in the mechanism. Some enzymes change shape significantly during the mechanism; in such cases, it is helpful to determine the enzyme structure with and without bound substrate analogues that do not undergo the enzymatic reaction.Not all biological catalysts are protein enzymes; RNA-based catalysts such as ribozymes and ribosomes are essential to many cellular functions, such as RNA splicing and translation. The main difference between ribozymes and enzymes is that RNA catalysts are composed of nucleotides, whereas enzymes are composed of amino acids. Ribozymes also perform a more limited set of reactions, although their reaction mechanisms and kinetics can be analysed and classified by the same methods.
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