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Name _____________________________________________________________ Cellular Respiration Review 1. Complete the following equation, which summarizes the degradative pathway known as aerobic respiration: ________ + ________ O2 6 ________ + 6 ________ + 38 __________ 2. a. b. c. d. e. f. g. h. i. j. Match the term to the correct description. Starting point for three energy-releasing pathways Main energy-releasing pathway for ATP formation Site of glycolysis Third and final stage of aerobic respiration; high ATP yield Oxygen is not the final electron acceptor Catalyze each reaction step in the energy releasing pathways Second stage of aerobic respiration; pyruvate is broken down into carbon dioxide and water Site of the second and third stages of the aerobic pathway The final electron acceptor in aerobic pathways The energy form that drives metabolic reactions _____ (1) _____ (2) _____ (3) _____ (4) _____ (5) _____ (6) _____ (7) _____ (8) _____ (9) _____ (10) 3. (1) (2) (3) (4) (5) Krebs cycle oxygen mitochondrion electron transfer phosphorylation enzymes ATP glycolysis aerobic respiration cytoplasm fermentation pathways and anaerobic electron transport Label each number of the illustration with the correct description. __________ __________ of mitochondrion __________ __________ of mitochondrion __________ __________ of mitochondrion __________ __________ of mitochondrion __________ (6) (7) (8) (9) (10) 4. a. b. c. 5. a. b. c. 6. a. b. c. 7. a. b. c. 8. a. b. c. 9. a. b. c. 10. a. b. c. 11. a. b. c. __________ __________ __________ __________ __________ __________ __________ Chemiosmosis occurs to form ATP molecules. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation Two carbon atoms in pyruvate leave as two CO2 molecules. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation Chemical reactions occur at transfer chains. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation Coenzyme A picks up a two-carbon acetyl group. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation Makes two turns for each glucose molecule entering glycolysis. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation Two NADH molecules form for each glucose entering glycolysis. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation Oxaloacetate forms from intermediate molecules. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation Named for a scientist who worked out its chemical details. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation 12. a. b. c. 13. a. b. c. 14. a. b. c. 15. a. b. c. 16. a. b. c. Occurs within the mitochondrion. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation Two FADH2 and six NADH form from one glucose molecule entering glycolysis. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation Hydrogens collect in the mitochondrion's outer compartment. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation Hydrogens and electrons are transferred to NAD+ and FAD. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation Two ATP molecules form by substrate-level phosphorylation. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation 17. Free oxygen withdraws electrons from the system and then combines with H+ to form water molecules. a. b. c. 18. a. b. c. 19. a. b. c. 20. a. b. c. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation No ATP is produced. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation Thirty-two or thirty-four ATPs are produced. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation Delivery point of NADH and FADH2 preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation 21. a. b. c. 22. a. b. c. 23. a. b. c. 24. a. b. c. Two pyruvates enter for each glucose molecule entering glycolysis. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation The carbons in the acetyl group leave as CO2. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation One carbon in pyruvate leaves as CO2. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation An electron transfer chain and channel proteins are involved. preparatory steps to Krebs cycle Krebs cycle electron transfer phosphorylation 25. Fill in the blanks. If oxygen is not present in sufficient amounts, the end product of glycolysis enters (1) ________ pathways. In some bacteria and muscle cells, pyruvate is converted into such products as (2) ________. In yeast cells it is converted into (3) ________ and carbon dioxide. Anaerobic pathways do not use oxygen as the final (4) ________ acceptor that ultimately drives the ATPforming machinery. Anaerobic routes must be used by many bacteria and protistans that live in an oxygen-free environment. (5) ________ precedes any of the fermentation pathways. During glycolysis, a glucose molecule is split into two (6) ________ molecules. Two energy-rich (7) ________ intermediate molecules form, and the net energy yield from one glucose molecule is two ATPs. In one kind of fermentation pathway, (8) ________ itself accepts hydrogen and electrons from NADH. Pyruvate is then converted to a three-carbon compound, (9) ________, during this process in a few species of bacteria and some animal cells. Human muscle cells can carry on lactate fermentation in times of oxygen depletion; this provides a low yield of ATP. In yeast cells, each pyruvate molecule from glycolysis forms an intermediate called (10) ________, as a gas, carbon dioxide, is detached from pyruvate with the help of an enzyme. This intermediate accepts hydrogen and electrons from NADH and is then converted to (11) ________, the end product of alcoholic fermentation. In both types of fermentation pathways, the net energy yield of two ATPs is formed during (12) ________. The reactions of the fermentation chemistry regenerate the (13) ________ needed for glycolysis to occur. Anaerobic electron transport is an energy-releasing pathway occurring among the (14) ________. For example, sulfate-reducing bacteria living in soil or water produce (15) ________ by stripping electrons from a variety of compounds and sending them through membrane transport systems. The inorganic compound (16) ________ (SO4=) serves as the final electron acceptor and is converted into foul-smelling hydrogen sulfide gas (H2S). 1. _________________________ 2. _________________________ 3. _________________________ 4. _________________________ 5. _________________________ 6. _________________________ 7. _________________________ 8. _________________________ 9. _________________________ 10. _________________________ 11. _________________________ 12. _________________________ 13. _________________________ 14. _________________________ 15. _________________________ 16. _________________________ 26. Is the statement true or false? Glucose is the only carbon-containing molecule that can be fed into the glycolytic pathway. 27. Is the statement true or false? Simple sugars, fatty acids, and glycerol that remain after a cell's biosynthetic and storage needs have been met are generally sent to the cell's respiratory pathways for energy extraction. 28. Is the statement true or false? Carbon dioxide and water, the products of aerobic respiration, generally get into the blood and are carried to gills or lungs, kidneys, and skin, where they are expelled from the animal's body. 29.Identify the process or substance indicated in the illustration below. (1) (2) (3) (4) (5) (6) (7) __________ __________ __________ __________ __________ __________ __________ __________ __________ __________ __________ 30. Choose the best answer. a. b. c. d. e. f. g. h. i. j. glucose glucose-6-phosphate glycogen fatty acids triglycerides PGAL acetyl-CoA amino acids glycerol proteins _____ (1) _____ (2) _____ (3) _____ (4) _____ (5) _____ (6) _____ (7) _____ (8) _____ (9) _____ (10) _____ (11) _____ (12) _____ (13) _____ (14) _____ (15) Fats that are broken down between meals or during exercise as alternatives to glucose Used between meals when free glucose supply dwindles; enters glycolysis after conversion Its breakdown yields much more ATP than glucose Absorbed in large amounts immediately following a meal Represents only 1 percent or so of the total stored energy in the body Following removal of amino groups, the carbon backbones may be converted to fats or carbohydrates or they may enter the Krebs cycle On the average, represents 78 percent of the body's stored food Between meals liver cells can convert it back to free glucose and release it Can be stored in cells but not transported across plasma membranes Amino groups undergo conversions that produce urea, a nitrogen-containing waste product excreted in urine Converted to PGAL in the liver; a key intermediate of glycolysis Accumulate inside the fat cells of adipose tissues, at strategic points under the skin A storage polysaccharide produced from glucose-6-phosphate following food intake that exceeds cellular energy demand (and increases ATP production to inhibit glycolysis) Building blocks of the compounds that represent 21 percent of the body's stored food A product resulting from enzymes cleaving circulating fatty acids; enters the Krebs cycle 31. Glycolysis would quickly halt if the process ran out of ________, which serves as the hydrogen and electron acceptor. a. b. c. d. 32. a. b. c. d. ADP NADP+ NAD+ H2O The ultimate electron acceptor in aerobic respiration is ________. carbon dioxide (CO2) NADH oxygen (1/2 O2) ATP 33. When glucose is used as an energy source, the largest amount of ATP is generated by the ________ portion of the entire respiratory process. a. b. c. d. glycolytic pathway acetyl-CoA formation Krebs cycle electron transfer phosphorylation 34. The process by which about 10 percent of the energy stored in a sugar molecule is released as it is converted into two small organic-acid molecules is ________. a. b. c. d. photolysis glycolysis fermentation the dark reactions 35. During which of the following phases of respiration is ATP produced directly by substrate-level phosphorylation? a. b. c. d. glycolysis Krebs cycle both a and b neither a nor b 36. What is the name of the process by which reduced NADH transfers electrons along a chain of acceptors to oxygen so as to form water and in which the energy released along the way is used to generate ATP? a. b. c. d. 37. a. b. c. d. 38. a. b. c. d. glycolysis acetyl-CoA formation the Krebs cycle electron transfer phosphorylation Pyruvic acid can be regarded as the end product of ________. glycolysis acetyl-CoA formation fermentation the Krebs cycle Which of the following is not ordinarily capable of being reduced at any time? NAD+ FAD oxygen, O2 water 39. ATP production by chemiosmosis involves ________. a. b. c. d. H+ concentration and electric gradients across a membrane ATP synthases both a and b neither a nor b 40. During the fermentation pathways, a net yield of two ATPs is produced from ________; the NAD+ necessary for ________ is regenerated during the fermentation reactions. a. b. c. d. the Krebs cycle; glycolysis glycolysis; electron transport phosphorylation the Krebs cycle; electron transport phosphorylation glycolysis; glycolysis 41. a. b. c. d. e. Match the following components of respiration to the list of words below. Glycolysis preparatory conversions prior to the Krebs cycle Fermentation Krebs cycle electron transport phosphorylation _____ (1) _____ (2) _____ (3) _____ (4) _____ (5) _____ (6) _____ (7) _____ (8) _____ (9) _____ (10) lactic acid, lactate NAD+ NADH Carbon dioxide is a product NADH NAD+ Pyruvate used as a reactant ATP produced by substrate-level phosphorylation Glucose Acetyl-CoA is either a reactant or a product Oxygen Water is a product 42. No matter what the source of energy may be, organisms must convert it to __________, a form of chemical energy that can drive metabolic reactions. 43. The main energy-releasing pathway is __________ respiration. 44. In the first of the three stages of aerobic respiration, one __________ is partially degraded to two pyruvate molecules. 45. By the end of the second stage of aerobic respiration, which includes the __________ cycle, __________ has been completely degraded to carbon dioxide and water. 46. Do the first two stages of aerobic respiration yield a high or low quantity of ATP? ________________________________________________________________________ 47. The third stage of aerobic respiration is called electron transport __________, which yields many ATP molecules. 48. Explain, in general terms, the role of oxygen in aerobic respiration. ______________________________________________________________________ _______________________________________________________________________ 49. Glycolysis occurs in the __________ of the cell. 50. Four ATP molecules are produced by __________-__________ phosphorylation for every two used during glycolysis. 51. Glycolysis produces __________ [number] NADH, __________ [number] ATP (net), and __________ [number] pyruvate molecules for each glucose molecule entering the reactions. 52. What happens to the CO2 produced during acetyl-CoA formation and the Krebs cycle? _________________________________________________________________________ 53. Account for the total net yield of 36 ATP molecules produced through aerobic respiration; that is, state how many ATPs are produced in glycolysis, the Krebs cycle, and the electron transfer chain. Glycolysis: ___________________ Krebs Cycle: __________________ Electron Transport: _____________ 54. In fermentation chemistry __________ molecules from glycolysis are accepted to construct either lactate or ethyl alcohol; thus, a low yield of __________ molecules continues in the absence of oxygen. 55. State which factors determine whether the pyruvate (pyruvic acid) produced at the end of glycolysis will enter into the alcoholic fermentation pathway, the lactate fermentation pathway, or the acetyl-CoA formation pathway. ____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ 56. List some sources of energy (other than glucose) that can be fed into the respiratory pathways. ____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ 57. Predict what your body would do to synthesize its needed carbohydrates and fats if you switched to a diet of 100 percent protein. ____________________________________________________________________________________ _____________________________________________________________________________________ _____________________________________________________________________________________ Cellular Respiration Answer Section SHORT ANSWER 1. ANS: C6H12O6 + 6O2 2. ANS: (1) (2) (3) (4) (5) (6) (7) (8) (9) (10) 6CO2 + 6H2O + 38 ATP g i h d f j a b c e 3-. ANS: 4. 5. 6. 7. 8. 9. 10. 11. 12. (1) (2) (3) (4) (5) (6) (7) (8) (9) (10) ANS: c ANS: a ANS: c ANS: a ANS: b ANS: a ANS: b ANS: b ANS: inner compartment of mitochondrion inner membrane of mitochondrion outer compartment of mitochondrion outer membrane of mitochondrion cytoplasm ATP oxygen FADH2 NADH electron transfer chain a, b, c 13. ANS: b 14. ANS: c 15. ANS: a, b 16. ANS: b 17. ANS: c 18. ANS: a 19. ANS: c 20. ANS: c 21. ANS: a 22. ANS: b 23. ANS: a 24. ANS: c 25. ANS: (1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (11) (12) (13) (14) (15) (16) 26. ANS: F 27. ANS: F 28. ANS: T fermentation (anaerobic) lactate ethanol electron Glycolysis pyruvate NADH pyruvate lactate acetaldehyde ethanol glycolysis NAD+ bacteria ATP sulfate 29. ANS: (1) fatty acids (2) glycerol (3) glycolysis (4) amino acids (5) Krebs cycle (6) acetyl-CoA formation (7) pyruvate 30. ANS: 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. (1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (11) (12) (13) (14) (15) ANS: c ANS: c ANS: d ANS: b ANS: c ANS: d ANS: a ANS: d ANS: c ANS: d e c d a c h e c b h i e c h g 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 54. 55. 56. 67. ANS: (1) c (2) a, b, d (3) b, c, d (4) c, e (5) b, c (6) a, d (7) a (8) b, d (9) e (10) a, e ANS: Pyruvic acid. ANS: aerobic ANS: Glucose molecule ANS: Krebs cycle; glucose ANS: Low. ANS: chain ANS: Final electron acceptor ANS: cytoplasm. ANS: Substrate-level. ANS: 2; 2; 2 ANS: It is exhaled as waste. ANS: Glycolysis: 2 ATP Krebs cycle: 2 ATP ETC: 32-34 ATP ANS: Pyruvic acid; ATP ANS: The presence of oxygen ANS: Proteins and lipids ANS: Convert proteins to acetyl co-A