Download Syllbus File - Al-Hussein Bin Talal University

Al-Hussein Bin Talal University
Princess Aisha Bint Al-Hussein College of Nursing & Health Sciences
Department of Laboratory Medical Sciences
1st semester 2016-2017
Clinical Immunology Syllabus
Instructor: Dr. Saleem Aladaileh
Office: level 2
Office hours: 11-12.30 M, W
Lecture-M,W, 9.30 -11 am, L1 201
Course Number : 0902142
Course Objectives: The overall course objective is to understand the cellular interactions and mechanisms of the immune
response, to become competent in analyzing data and scientific research articles and to become experienced in
communicating scientific data in written form.
Content:
Clinical Immunology Syllabus ................................................................................................................................................... 1
Overview of Immunology ......................................................................................................................................................... 2
Brief History and Introduction of Immunology ................................................................................................................ 2
Immune Organs, Tissues, and Cells .................................................................................................................................. 2
Antigens .................................................................................................................................................................................... 2
Immunoglobulin........................................................................................................................................................................ 3
Complement System, Cytokines, CD and AM (2 h ................................................................................................................... 3
Complement System ......................................................................................................................................................... 3
Cytokines, CD and AM (0.5 h) ........................................................................................................................................... 4
MHC ........................................................................................................................................................................................... 4
Immune Cells............................................................................................................................................................................. 4
Antigen presenting cells and antigen processing and presentation ........................................................................................ 5
Immune Response .................................................................................................................................................................... 5
Innate immunity, Immunotolerance and Immunoregulation (2 h) ................................................................................ 5
Hypersensitivity ........................................................................................................................................................................ 6
Immunoprophylaxis and Immunotherapy ............................................................................................................................... 6
[Text book ]
Riott’s Essential Immunology
kuby immunology
1
Overview of Immunology
Brief History and Introduction of Immunology
[Learning Objectives]
1. To master the modern concept of immunity
2. To learn about the composition and features of innate immunity and acquired immunity
3. To be familiar with the basic function of immunity
4. To learn about clonal selection theory
5. To learn about the brief history and recent developments in immunology, and its status in medicine
[Contents]
1. The basic aspects of immunity: concept, function, classification of immunity
2. Brief history of immunity (experience period, classical period, modern period).
3. The main content of clonal selection theory
4. The difference between innate immunity and acquired immunity, the composition of innate immunity,
the main features of specific immunity
5. The category of immunological study and the branch of immunology
6. The status, development, and current trend of Immunity in Medicine and Biology
Immune Organs, Tissues, and Cells
[Learning Objectives]
1. To master the composition of immune system, function of central immune organs and the peripheral
immune organs
2. To learn about the development, differentiation, maturation of T Cells and B Cells in immune organs
[Contents]
1. The summary of immune organs
2. Central immune organs (bone marrow, thymus, bursa of fabricius).
3. Peripheral immune organs (spleen, lymph nodes)
4. Lymphoid tissues (mucosa associated lymphoid tissue and skin associated lymphoid tissue)
5. Lymphocyte recirculation and its significance
Antigens
[Learning Objectives]
1. To master the basic concept of antigen, immunogen, and hapten
2. To master the common antigens, cross reaction and its clinical significance
3. To master the classification of antigens and the important antigens in medicine
4. To be familiar with the concept of mitogens, superantigens, and adjuvants
[Contents]
1. Important concepts
1.1. Antigen, Immunogen, hapten
1.2. Antigenicity and Immunogenicity
2. Specificity and foreigness of antigen
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2.1. Antigenic determinants (epitopes), antigen-binding valence?
2.2. Patterns of antigenic epitopes
2.3. Functions of epitopes
2.4. Common antigens and cross reaction
3. Main factors influencing immunogenicity of antigen
4. Classification of antigens and the important antigens in medicine
5. Superantigens and adjuvant
Immunoglobulin
[Learning Objectives]
1. To master the concept of antibody (immunoglobulin)
2. To master the basic structure, function, and fragments of antibody
3. To master the characteristics and functions of antibody classes (IgM, IgD, IgG, IgA, and IgE)
4. To be familiar with the serological types of antibody
5. To learn about the genetics of antibody
6. To learn about the artificial production of antibody (polyclonal and monoclonal antibodies)
[Contents]
1. Concept of antibody and immunoglobulin
2. Basic structure, function, and fragments of antibody
3. Characteristics and functions of antibody classes (IgM, IgD, IgG, IgA, and IgE)
4. Biological functions of antibody
5. Serological types of antibody: isotype, allotype and idiotype
6. Antibody genetics
7. Artificial production of antibodies (polyclonal, monoclonal and genetic engineering antibodies)
Complement System, Cytokines, CD and AM
Complement System
[Learning Objectives]
1. To master the definition of complement system
2. To be familiar with the physical and chemical properties of complement system
3. To master the pathways of complement activation, coherence, and differences between the pathways
4. To master the biological functions of complement system
5. To learn about the regulatory mechanism of complement activation, components, and functions of
complement receptors
[Contents]
1. Discovery , definition, physical and chemical properties of complement system
2. Complement components and denomination
3. Activation of the complement system—three pathways: classical pathway, Mannan-binding lectin
pathway, alternative pathway; MAC; Comparison of three pathways
4. Regulation of the activation of the complement system: self-regulation and roles of the regulatory
factor
5. Complement receptors: components, distribution, and functions
3
6. Biological functions of the complement system: mediating cytolysis; opsonization; activation of
inflammation; clearance of the immune complex; regulation of immune response
Cytokines, CD and AM
[Learning Objectives]
1. To master the definition, classification, and common properties of cytokines
2. To be familiar with the biological functions of interleukins (such as IL-1, 2, 4, 6, 10)
3. To be familiar with the main family members and functions of interferon, tumor necrosis factor, colony
stimulating factor, chemokines
4. To master the definitions of the CD and the AM
[Contents]
1. Definition and common properties of cytokine
2. Classification and key effects of cytokines: interleukin, interferon, tumor necrosis factor, colony
stimulating factor, chemokine
3. The roles of cytokine receptors
4. Definitions of the cluster of differentiation (CD) and the adhesion molecule (AM)
MHC
[Learning Objectives]
1. To master the concept of major histocompatibility complex
2. To be familiar with the nomenclature of MHC
3. To be familiar with the composition and classification of HLA gene locus
4. To master the structural characteristics, distribution and biological function of human HLA-I and II
antigens
5. To be familiar with the genetic characteristics of human HLA complex
6. To learn about the significance of HLA in medicine
[Contents]
1. History, concept and nomenclature of MHC
2. Genetic constitution of human HLA complex: HLA class I, class Ⅱand class III gene
3. Structure and distribution characteristic of HLA molecule
4. Biological function of human HLA molecules: protein antigen presentation, immune response regulation,
T cell differentiation
5. Genetic characteristics and typing of HLA complex: genetic characteristics (Haplotype, codominant
inheritance, high polymorphism, linkage disequilibrium), typing technology (serologicaltyping, cytological
typing , DNA typing )
6. Significance of HLA in medicine: relevance to disease, organ transplantation
Immune Cells (2 h)
[Learning Objectives]
1. To learn about the characteristics of hematopoietic stem cell, development of T and B lymphocyte
2. To master main surface maker, classification and biological functions of T lymphocyte
3. To master main surface maker, classification and biological functions of B lymphocyte
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4. To be familiar with surface maker, recognition mechanism and biological functions of natural killer cell
[Contents]
1. The main characteristics of hematopoietic stem cell
2. T lymphocyte: development, main surface maker, classification and biological functions
3. B lymphocyte: development, main surface maker, classification and biological functions
4. Natural killer cell: features, biological activities (recognition mechanism, pattern of killing target cells,
ADCC, functions)
Antigen presenting cells and antigen processing and presentation
[Learning Objectives]
1 To master the types and functions of APCs
2 To master the presentation of endogenous and exogenous antigens
3 To learn about antigen presentation by CD1 molecule
[Contents]
1 Concept and the types of APCs, including professional and non-professional APCs
2 Characteristics, development, types and functions of professional APCs (DCs, macrophages, and B cells)
3 Definitions of endogenous and exogenous antigen
4 The class II MHC pathway and the class I MHC pathway for antigen presentation
5 The CD1 pathway to present lipid antigens
Immune Response
[Learning Objectives]
1. To master concept, types, places and processes of immune response
2. To master antigen recognition of T and B lymphocytes and the process of T and B lymphocyte activation,
proliferation and effectors
3. To be familiar with characteristics and differences of immune response to TD-Ag and TI-Ag
4. To master effects of cell-mediated immune response and humoral immune response
5. To master the concept and characteristics of primary and secondary immune responses
6. To be familiar with the characteristics of cell-mediated immune response, mechanisms of CTL killing of
target cell
[Contents]
1. Concept, types, places and processes of immune response
2. B-cell-mediated immune response: characteristics and processes of B1 cell response to TI-Ag, B2 cell
response to TD-Ag (antigen recognition, T and B cell activation, proliferation, differentiation and
effector), general rule of Ab producing (primary and secondary response)
3. T-cell mediated immune response: TDTH-mediated inflammation, CTL-mediated cytotoxicity, biological
effects of cell-mediated immunity
Innate immunity, Immunotolerance and Immunoregulation
Innate immunity
[Learning Objectives]
1. To master the concept of innate immunity
2. To be familiar with components of innate immunity
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3. To be familiar with the comparisons and the relationship of innate and adaptive immunity
[Contents]
1. Concept and characteristics of innate immunity
2. Components of innate immunity
3. Comparisons of innate and adaptive immunity
4. Role of innate immunity to stimulating adaptive immune responses
Immunotolerance and Immunoregulation
[Learning Objectives]
1. To master the concept of immunotolerance
2. To be familiar with mechanisms of central and peripheral tolerance
3. To learn about the factors influencing immunotolerance
4. To learn about the different levels of immunoregulation
[Contents]
1. Concept of immunotolerance, phenomena of immunotolerance (natural and acquired
immunotolerance)
2. Mechanisms of immunotolerance formation
3. Biological significance of immunotolerance
4. Regulation effects of Ag, Ab, immune complex, immune cells, cytokines, idiotype-network,
Immunoneuroendocrine networks and genetic control to immune response
Hypersensitivity
[Learning Objectives]
1. To master concept, classification of hypersensitivity
2. To mater characteristics and the mechanisms of each type of hypersensitivity.
3. To be familiar with prevention and treatment of type I hypersensitivity
4. To learn about common diseases of four types of hypersensitivity in clinic
[Contents]
1. Concept and classification of hypersensitivity
2. Characteristics, mechanisms of typeⅠhypersensitivity, common diseases in clinic and the principles of
prevention and treatment
3. Characteristics, mechanisms of typeⅡ hypersensitivity, common diseases in clinic
4. Characteristics, mechanisms of type Ⅲ hypersensitivity, common diseases in clinic
5. Characteristics, mechanisms of type Ⅳ hypersensitivity, common diseases in clinic
6.
Immunoprophylaxis and Immunotherapy
[Learning Objectives]
1. To master concept of artificial active immunization, artificial passive immunization, adoptive
immunotherapy
2. To be familiar with vaccines and antibodies commonly used in clinic, methods of adoptive
immunotherapy
3. To learn immunopotentiators and immunosuppressants commonly used in clinic
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[Contents]
1. Concept of artificial active immunization, types of vaccines
2. Concept of artificial passive immunization, types of immunoproducts for artificial passive immunization
3. Concept of adoptive immunization, commonly used methods of adoptive immunization (LAK, TIL,
cytokine gene transfection of immune cells)
4. Types of immunopotentiators and immunosuppressants commonly used in clinic (immunopotentiators:
IL-2 , IFN- γ, MDP, ISO, BCG, CP; immunosuppressants: CsA, FK-506, glucocorticoids, cyclophosphamide,
anti-CD3-antibody, anti-thymocyte-serum,etc)
Grading:
1st exam
2nd exam
Final exam
Laboratory
20
20
45
15
7