Download 432W9EX1

BI432
Immunology
Exam #1
Winter, 1999
1
Name:
1. Indicate to which branch(es) of the immune system the following statements apply, using H for the humoral
branch, CM for the cell-mediated branch, B for both branches and N for neither branch.
a)
Involves class I MHC molecules
f)
involves cytotoxic T cells
b)
responds to viral infections
g)
involves B cells
c)
involves T helper cells
h)
involves CD8+ cells
d)
involves processed antigen
i)
responds to extracellular bacterial
e)
most likely responds following an
organ transplant
infection
j)
kills virus-infected self cells
2. List the primary lymphoid organs and summarize the function of each in the immune response.
3. For each of the following statements, indicate whether it is true only of B cell epitopes (B), only of T cell
epitopes (T), or both types of epitopes (BT) within a large antigen
a)
they ALWAYS consist of a linear sequence of amino acids
b)
they generally are located in the interior of a protein antigen
c)
they generally are located on the surface of a protein antigen
d)
they lose their immunogenicity when a protein antigen is subjected to heat denaturation
e)
immunodominant epitopes are determined in part by an individual’s MHC molecules
f)
they are present only in protein antigens
g)
multiple different epitopes may occur in the same antigen
h)
their immunogenicity may depend on the three dimensional structure of the antigen
i)
the immune response to them may be enhanced by Freund’s complete adjuvant.
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4. Describe the products of treatment of an IgG with each of the following reagents:
mercaptoethanol
pepsin
5. Identify each of the indicated regions of the molecule in the figure immediately below. Label the location of
the CDR with the letter “C”, the location of Ig fold #3 with the number 3, and the hinge region with the letter
“H”.
6. According to the clonal selection theory, all the immuoglobulin molecules on a single B cell have the same
antigenic specificity. Explain why the presence of both IgM and IgD on the same B cell does not violate the
unispecificity implied by clonal selection.
BI432
Immunology
Exam #1
Winter, 1999
3
Name:
7. For each immunoglobulin isotype select the description(s) listed below (a-i) that are true about that isotype.
Each description may be used once, more than once, or not at all; more than one description may apply to some
isotypes.
IgA:
IgD:
IgE:
IgG:
IgM:
a) secreted form is a pentamer of the basic H2L2 unit
b) binds to Fc receptors on mast cells
c) multimeric forms have J chain
d) present on the surface of mature, unprimed B cells
e) the most abundant isotype in serum
f) major antibody in secretions such as saliva, tears, and breast milk
g) present on the surface of immature B cells
h) plays primary role in protecting against pathogens that invade through the gut or respiratory mucosa
i) multimeric forms may contain a secretory component
8. List, in increasing sensitivity, three qualitative immunoassays.
9. You perform an Ouchterlony assay in which the central well contains goat antiserum against the F(ab’)2
fragment of pooled mouse IgG and the surrounding wells (A-F) contain six different test antigens. The resulting
precipitin pattern is shown below.
Based on this pattern, indicate which well contains each of the following test antigens:
a mixture of lambda heavy chains and kappa light chains
a) Fab fragment from an IgG myeloma
c) gamma heavy chains
protein
d) kappa light chains
b) Fc fragment from IgG myeloma
e) lambda light chain
protein
10. Identify each of the cells, the types of Ig expressed, and the molecular events that occur at the steps in B-cell
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development diagrammed in the following figure.
11. For each immunoglobulin heavy-chain or light-chain DNA, mRNA, of polypeptide described in the phrases
a through g below, select the corresponding schematic diagram (1 to 9) from the figure below.
BI432
Immunology
Exam #1
Winter, 1999
5
Name:
12. List seven mechanisms that have been shown to generate antibody diversity in the mouse, and describe the
manner in which any four of these mechanisms operate to produce antibody diversity.
winword\immunol\432w9ex1
6/23/2017
BI432
Immunology
Exam #1
Winter, 1999
7
Name:
1. Indicate to which branch(es) of the immune system the following statements apply, using H for the humoral
branch, CM for the cell-mediated branch, B for both branches and N for neither branch.
a)
Involves class I MHC molecules
f)
involves cytotoxic T cells
b)
responds to viral infections
g)
involves B cells
c)
involves T helper cells
h)
involves CD8+ cells
d)
involves processed antigen
i)
responds to extracellular bacterial
e)
most likely responds following an
infection
organ transplant
j)
kills virus-infected self cells
2. List the secondary lymphoid organs and summarize the function of each in the immune response.
3. Complete the following table:
CHARACTERISTIC
B CELLS
T CELLS
Interaction with antigen
binding of soluble antigen
involvement of MHC molecules
chemical nature of antigens
epitope properties
4. Describe the products of treatment of an IgG with each of the following reagents:
mercaptoethanol
papain
5. Identify each of the indicated regions of the molecule in the figure immediately below. Label the location of
winword\immunol\432w9ex1
6/23/2017
the CDR with the letter “C”, the location of Ig fold #3 with the number 3, and the hinge region with the letter
“H”.
6. According to the clonal selection theory, all the immuoglobulin molecules on a single B cell have the same
antigenic specificity. Explain why the presence of both IgM and IgD on the same B cell does not violate the
unispecificity implied by clonal selection.
BI432
Immunology
Exam #1
Winter, 1999
9
Name:
7. For each immunoglobulin isotype select the description(s) listed below (a-i) that are true about that isotype.
Each description may be used once, more than once, or not at all; more than one description may apply to some
isotypes.
Isotypes:
IgA:
IgD:
IgE:
IgG:
IgM:
a) secreted form is a pentamer of the basic H2L2 unit
b) binds to Fc receptors on mast cells
c) multimeric forms have J chain
d) present on the surface of mature, unprimed B cells
e) the most abundant isotype in serum
f) major antibody in secretions such as saliva, tears, and breast milk
g) present on the surface of immature B cells
h) plays primary role in protecting against pathogens that invade through the gut or respiratory mucosa
i) multimeric forms may contain a secretory component
8. List, in decreasing sensitivity, three quantitative immunoassays.
9. You perform an Ouchterlony assay in which the central well contains goat antiserum against the F(ab’)2
fragment of pooled mouse IgG and the surrounding wells (A-F) contain six different test antigens. The resulting
precipitin pattern is shown below.
Based on this pattern, indicate which well contains each of the following test antigens:
a mixture of lambda heavy chains and kappa light chains
a) Fab fragment from an IgG myeloma
c) gamma heavy chains
protein
d) kappa light chains
b) Fc fragment from IgG myeloma
e) lambda light chain
protein
winword\immunol\432w9ex1
6/23/2017
10. Identify each of the cells, the types of Ig expressed, and the molecular events that occur at the steps in B-cell
development diagrammed in the following figure.
11. For each immunoglobulin heavy-chain or light-chain DNA, mRNA, of polypeptide described in the phrases
a through g below, select the corresponding schematic diagram (1 to 9) from the figure below.
12. List seven mechanisms that have been shown to generate antibody diversity in the mouse, and describe the
manner in which any four of these mechanisms operate to produce antibody diversity.