Download BIO 105 Lab 1 Gametogenesis BGee 150123

Biology 105 – Human Biology
Session:
Spring 2015
Section:
55244 / 61816 4 Units
Class Location:
Days / Time:
UVC1, 3 and 7 St. Helena
F 9:00 AM – 11:50 AM LEC
F 1:00 PM – 3:50 PM LAB 55244
M 9:00 AM – 11;50 AM LAB 61816
Laboratory Report
Instructor:
RIDDELL
Student Author Name:
Team Members Name(s):
Student Author ID #:
Team Members ID #’(s):
Lab Assignment #:
Team Name:
Lab Assignment Title:
Date:
Purpose / Objective(s):




Observe Cheek Cells
Observe Human Gametogenesis in prepared MS Slides via standard Light Microscopy
Survey and capture illustrations and photos from the internet of the above
Measure Cell and Structure Size utilizing Field of View Calculator
Hypothesis:
NA
Materials / Subjects / Specimens:
Specimen of cheek cells form self
Prepared MS slides featuring a cross-section of:
 Sperm Smear Human

 Ovary Maturing, Follicle Human

.
Methods / Tools / Instrumentation / Procedures:
1. Prepared a MS sample of cheek cells from self and observed via standard Light Microscopy
2. Standard Light microscope with 100, 400 and 1000 Magnification
3. View cheek cells at 100X and 400 X
4. View Human sperm smear, and view ovary maturing, and follicle sections in human ovary
873999317
Biology 105 – Human Biology
Session:
Spring 2015
Section:
55244 / 61816 4 Units
Class Location:
Days / Time:
UVC1, 3 and 7 St. Helena
F 9:00 AM – 11:50 AM LEC
F 1:00 PM – 3:50 PM LAB 55244
M 9:00 AM – 11;50 AM LAB 61816
Instructor:
Laboratory Report
RIDDELL
Results

Viewing the target specimens and structures with the standard light microscopes required skill
and patience.




Gametogenesis tissues and cells are presented in Table 1
o Male and Female structures
Sample measurements from the FOV Calculator are presented in Table 2
Table 3 lists the size measurements for the cells and structures of Gametogenesis
Figure 1 compares the cells and structure sizes of Gametogenesis
Analysis / Discussion:

 …
 ….
 ….
 …..
Research
Events of Meiosis
Interphase
Cell growth, cell doubling DNA.
Chromosomes duplicate
Chromatin inside nuclear envelope, and centriole pair with another centriole to make
centrioles
Prophase
Centromere attaches to the spindles and the centromere begin to form. Spindles stretch
and spread apart and the centromere begin to bind to the spindles. In the process of
synapsis the two sister chromatids form and attach together
Metaphase
The chromosome tetrads are aligned on the plate. The centrioles are at opposite sides of
the pole. Spindle fibers are aligned around the entire cell.
Anaphase
Daughter chromosomes begin to split apart and leave to opposite ends of the cell; the
spindles also start to break apart. The centrioled remain at opposite ends of the poles.
Telophase
Cleavage furrow is in between the cells and the two cells are beginning to form a formation
to leave into the next stage of Cytokinesis. This is when the cell officially forms into two
cells and the two sister chromatids separate. Then the cycle repeats itself into mitosis, and
in the very end we are left with four duplicate cells.
873999317
Biology 105 – Human Biology
Session:
Spring 2015
Section:
55244 / 61816 4 Units
Class Location:
Days / Time:
UVC1, 3 and 7 St. Helena
F 9:00 AM – 11:50 AM LEC
F 1:00 PM – 3:50 PM LAB 55244
M 9:00 AM – 11;50 AM LAB 61816
Laboratory Report
Instructor:
RIDDELL

873999317
Biology 105 – Human Biology
Session:
Spring 2015
Section:
55244 / 61816 4 Units
Class Location:
Days / Time:
UVC1, 3 and 7 St. Helena
F 9:00 AM – 11:50 AM LEC
F 1:00 PM – 3:50 PM LAB 55244
M 9:00 AM – 11;50 AM LAB 61816
Laboratory Report
Instructor:
RIDDELL
Conclusions/Further Considerations:



………
………..
………….
References
1. http://webnt.calhoun.edu/distance/internet/natural/anatomy/EndoRepro/ovary4X.html
2. http://faculty.baruch.cuny.edu/jwahlert/bio1003/mitosis.html
873999317
Biology 105 – Human Biology
Session:
Spring 2015
Section:
55244 / 61816 4 Units
Class Location:
Days / Time:
UVC1, 3 and 7 St. Helena
F 9:00 AM – 11:50 AM LEC
F 1:00 PM – 3:50 PM LAB 55244
M 9:00 AM – 11;50 AM LAB 61816
Instructor:
Laboratory Report
RIDDELL
ATTACHMENTS:
Table 1: Illustrations and Micrographs of Each Mitosis Stage
Stage
Key Event(s)
Notes /
Description / Slide
Specimen
Interphase
Chromosomes
begin to form
Cell growth
Prophase
Chromatids pair
up and make
chromosomes
Metaphase
Chromosomes
shre DNA and
line up in a line
Replicated
chromosomes,
consisting of two
sister chromatids
held together at the
centromere
chromosomes
assemble on the
equatorial plate
Anaphase
Chromosomes
split apart
Sister chromatids
separate from each
other and move to
opposite poles of the
cell.
Telophase
&
Cytoknesis
All DNA is
doubles and split
into their cells
Cells are separated
Illustration/
Diagram
Photo
Micrograph
Web Reference for white fish and Onion Root cells:
http://faculty.baruch.cuny.edu/jwahlert/bio1003/mitosis.html
873999317
Biology 105 – Human Biology
Session:
Spring 2015
Section:
55244 / 61816 4 Units
Class Location:
UVC1, 3 and 7 St. Helena
F 9:00 AM – 11:50 AM LEC
Days / Time:
F 1:00 PM – 3:50 PM LAB 55244
M 9:00 AM – 11;50 AM LAB 61816
Instructor:
Laboratory Report
RIDDELL
Table 2: Illustration and Photo Micrographs of Meiosis 1 and Meiosis 2
Stages
Meiosis
Key event(s)
Notes/Description/
slide specimen
Prophase
Phase 1
Cleavage
furrow is
present in
between both
cells
Cells uncoil and
nuclear envelope
forms
Metaphase
Phase 2
Spindles align
with each other
Chromosomes pair
up with each other in
a straight line
anaphase
Phase 3
Spindles
spread apart
and
chromosomes
split
Sister chromatids
separate in
anaphase two, as for
anaphase one where
they stay together
Telophase 2
Phase 4
Cleavage
furrow begins
to form
After it forms then it
separates and forms
around the cell.
Illustration
Meiosis II
Photo-Micrograph
Meiosis II
873999317
Biology 105 – Human Biology
Session:
Spring 2015
Section:
55244 / 61816 4 Units
Class Location:
Days / Time:
UVC1, 3 and 7 St. Helena
F 9:00 AM – 11:50 AM LEC
F 1:00 PM – 3:50 PM LAB 55244
M 9:00 AM – 11;50 AM LAB 61816
Laboratory Report
Instructor:
RIDDELL
Table 3: Illustrations/Notes of cells- (Human Gametogenesis , Testis, Sperm, and Ovary
Table 3 Gametogenesis and Human Development
http://webnt.calhoun.edu/distance/internet/natural/anatomy/EndoRepro/ovary4X.html
873999317